Genescan 3.7 analysis software

Manufactured by Thermo Fisher Scientific

Sourced in United States

GeneScan 3.7 is a software suite designed for the analysis of DNA fragment data generated by Thermo Fisher Scientific's genetic analysis instruments. The software provides tools for sizing, quantifying, and comparing DNA fragments, enabling researchers to effectively analyze and interpret their genetic data.

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Lab products found in correlation

Qiaamp dna tissue kit, by Qiagen (1 mentions) Abi 3130xl gene analyzer, by Thermo Fisher Scientific (1 mentions) Abi 310 genetic analyser, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

GeneScan Analysis software 3.1 GeneScan Analysis software GeneScan Analysis GeneScan software Genescan 3.7 software

2 protocols using genescan 3.7 analysis software

Microsatellite Instability Analysis in Paraffin-Embedded Tissues

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DNA was extracted from paraffin-embedded tissue samples using the QIAamp DNA Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. MSI analysis was performed on six mononucleotide repeat markers (BAT-25, BAT-26, NR-21, NR-24, NR-27, and MONO-27; Table 2) using a Microsatellite Instability Detection Kit (Microread Gene Technology Co., Ltd, Beijing, China). To detect potential specimen contamination, pentanucleotide repeat markers (Penta C and Penta D) and a sex locus marker (amelogenin) were also analyzed for background confirmation. Fluorescently labeled primers were used in the PCR assay, and results were analyzed on an ABI 3130XL gene analyzer with the GeneScan 3.7 analysis software (Applied Biosystems, Foster City, California, USA). Samples were considered MSI-H when two or more of the markers displayed MSI. Samples with MSI at only one marker were considered MSI-L, and those with no MSI were considered MSS.

Ren X.Y., Song Y., Wang J., Chen L.Y., Pang J.Y., Zhou L.R., Shen S.J., Cao X., Wang Y.X., Shao M.M., Liang Z.Y., Sun Q, & Wu H.W. (2021). Mismatch Repair Deficiency and Microsatellite Instability in Triple-Negative Breast Cancer: A Retrospective Study of 440 Patients. Frontiers in Oncology, 11, 570623.

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Microsatellite Markers for C. parapsilosis Identification

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Four C. parapsilosis-specific microsatellite markers were used, designated as CP1, CP4, CP6 and B, and PCR amplification was performed following the methodology developed and described previously [23] .
After PCR, denaturated samples were run in an ABI 310 Genetic Analyser (AB Applied Biosystems), the PCR product size was determined by the GeneScan 3.7 Analysis software and alleles were designated by their sizes in base pairs [23] .

Sabino R., Sampaio P., Rosado L., Videira Z., Grenouillet F, & Pais C. (2015). Analysis of clinical and environmental Candida parapsilosis isolates by microsatellite genotyping--a tool for hospital infection surveillance. Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases, 21(10).

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