The concentrations of TNF-α and MCP-1 in serum were measured using Rat TNF-α and MCP-1 Immunoassay kit (MyBioSource, Inc., San Diego, USA) according to the manufacturer's protocols Rna Isolation And Quantitative Real-time Pcr Total RNA of the WAT, BAT, and hypothalamus was extracted with Trizol Reagent. Synthesized complementary DNA (cDNA) via reverse transcription was ampli ed by real-time PCR on a quantitative PCR System. PCR ampli cation was conducted with a uorescence thermal cycler system using SYBR green kit and rat speci c primer sequences for GRP78, TNF-α, and MCP-1 as target genes and β-actin as the housekeeping gene. All of the mentioned experiments were performed with experts who were blinded to treatment groups
Rat tnf α and mcp 1 immunoassay kit
Manufactured by MyBioSource
Sourced in United States
The Rat TNF-α and MCP-1 Immunoassay kit is a laboratory tool designed to detect and quantify the levels of Tumor Necrosis Factor-alpha (TNF-α) and Monocyte Chemoattractant Protein-1 (MCP-1) in rat samples. This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) technique to provide accurate and reliable measurements of these two cytokines.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rat tnf α and mcp 1 immunoassay kit
1
Analysis of Inflammatory Markers in Rat Tissues
2
Serum Cytokine and Gene Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentrations of TNF-α and MCP-1 in serum were measured using Rat TNF-α and MCP-1 Immunoassay kit (MyBioSource, Inc., San Diego, USA) according to the manufacturer's protocols RNA isolation and quantitative real-time PCR Total RNA of the WAT, BAT, and hypothalamus was extracted with Trizol Reagent. Synthesized complementary DNA (cDNA) via reverse transcription was ampli ed by real-time PCR on a quantitative PCR System. PCR ampli cation was conducted with a uorescence thermal cycler system using SYBR green kit and rat speci c primer sequences for GRP78, TNF-α, and MCP-1 as target genes and β-actin as the housekeeping gene. All of the mentioned experiments were performed with experts who were blinded to treatment groups
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!