Seeds from the Arabidopsis FLIPPi-30m and FLIPPi-4µ lines, were surface-sterilized using vapor-phase seed sterilization and sown on plates with ½ MS media (Duchefa Biochemie, Haarlem, The Netherlands) supplemented with 0.5% sucrose and adjusted to pH 5.8. Prior to germination, seeds were stratified for 3 days in a cold room at 4°C in the dark to promote uniform germination. MS plates were placed in a growth chamber with 16/8 h light/dark cycle, with 125 µmol m−2 s−1 white light, 22/20 °C light/dark temperature, and 70% relative humidity. Eight- to 12-day-old seedlings were used for FRET analysis with Pi buffer perfusions in roots.
Ms media
Manufactured by Duchefa Biochemie
MS media is a type of plant growth media used for tissue culture and micropropagation. It provides the essential nutrients and vitamins required for the growth and development of plant cells, tissues, and organs in a controlled laboratory environment.
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Lab products found in correlation
5 protocols using ms media
1
Arabidopsis FLIPPi for FRET Analysis
2
Arabidopsis thaliana Growth and Genotyping
3
Seed Germination and Plant Growth Protocol
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Seeds were sowed on soil (CL TON KOKOS, CLASSIC Profisubstrat, Einheits erde) in square plastic pots (8 cm × 8 cm × 7 cm, width × depth × height) watered with Solbac solution 1:400 (Andemat Biocontrol). After stratifying for 3–5 d plants were grown in 16:8-light/dark cycles in a walk-in incubator equipped with white and red LEDs, photosynthetic photon flux density (PPFD) = 100 µmol m−2 s−1, at 22°C.
For experiments involving light reflection seeds were sowed on 0.8% (w/v) water-agar plates, stratified for 3–5 d and grown for 4 d in light until they were de-etiolated. A square of aluminum foil was placed over the soil, and four small holes (2–3 mm diameter) were done on each corner. The holes were filled with moistened soil, and seedlings were transplanted inside.
InFigure 1, A, B, and D , seeds were surface-sterilized and sowed in square transparent plates (10 × 10 × 2 cm3) with half MS media (Duchefa) plus 0.8% agar (Roth).
For experiments involving light reflection seeds were sowed on 0.8% (w/v) water-agar plates, stratified for 3–5 d and grown for 4 d in light until they were de-etiolated. A square of aluminum foil was placed over the soil, and four small holes (2–3 mm diameter) were done on each corner. The holes were filled with moistened soil, and seedlings were transplanted inside.
In
4
Arabidopsis thaliana Transgenic Lines for Developmental Studies
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The present study was conducted on Arabidopsis thaliana, ecotype Columbia (Col-0), and the transgenic lines pWOX5::erGFP34 (link), pQC25::GUS35 , pDR5rev::GFP36 (link), pCYCB1;1::GUS48 (link) have been described previously. Seeds were sterilized in 70% ethanol for 1 min, followed by 25% commercial bleach solution for 5 min, and washed several times with distilled water. Seeds were sown in 1/2 MS media (Duchefa, The Netherlands) containing 1% sucrose, 0.5 g/L 4-morpholineethanesulfonic acid (Amresco, USA), and 0.8% agar (pH 5.7). After stratification for at least 2 days at 4 °C in darkness, the seeds were sown in the media, and the plates were placed vertically under continuous 80 μmol m−2 s−1 light at 22 °C in an environmentally controlled growth room (Korea Instruments, Korea).
5
Aseptic Cultivation of D. binata
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Plants of D. binata were cultivated aseptically on MS media (Duchefa, The Netherlands) supplemented with 2% (w/v) sucrose and 0.8% (w/v) agar at 24 °C ±2 °C with 16/8 photoperiod and light intensity of 50 μmol m -2 s -1 .
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