Crude methanol extract (4 g) was loaded on to a 600 mm  30 mm silica gel (60-120 mesh) column and eluted successively by passing various solvents (150 mL each) of increasing polarities such as petroleum benzene, chloroform, acetone and methanol. The fraction obtained in methanol (AMF), which was found most effective in scavenging free radicals, was used for further studies. Chemicals 7,12-Dimethylbenz[a]anthracene (DMBA) was purchased from Sigma Chemicals (St. Louis, MO). DMBA was dissolved at a concentration of 100 mg/100 mL acetone. Croton oil was also dissolved in acetone to give a solution of 1% dilution. Nutrient broth was purchased from Hi-media Laboratories, Mumbai, India. Agar agar, L-histidine, biotin, glucose-6-phosphate, dimethyl sulphoxide and NaN 3 were obtained from Sisco Research Laboratories, Mumbai, India. NPDA was purchased from Sigma Chemicals. All other chemicals used were of the analytical grade.
Dimethyl sulphoxide
Manufactured by Sisco Research Laboratories
Sourced in India
Dimethyl sulphoxide is a colorless, odorless, and polar aprotic solvent. It has a high boiling point and is miscible with water and a wide range of organic solvents. Dimethyl sulphoxide is commonly used as a solvent in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Glucose 6 phosphate (g6p), by Sisco Research Laboratories (1 mentions)
Nutrient broth, by HiMedia (1 mentions)
N nitrosodiethylamine, by Merck Group (1 mentions)
May gr nwald stain, by Merck Group (1 mentions)
Curcumin, by Merck Group (1 mentions)
Ethidium bromide, by Sisco Research Laboratories (1 mentions)
Agarose, by Sisco Research Laboratories (1 mentions)
Triton x, by Sisco Research Laboratories (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Sisco Research Laboratories (1 mentions)
Giemsa stain, by Merck Group (1 mentions)
Pageruler plus prestained protein ladder, by Thermo Fisher Scientific (1 mentions)
Methanol, by Sisco Research Laboratories (1 mentions)
Acrylamide, by Sisco Research Laboratories (1 mentions)
Coomassie brilliant blue r 250, by Sisco Research Laboratories (1 mentions)
Triton x 100, by Sisco Research Laboratories (1 mentions)
Diethyl ether, by Sisco Research Laboratories (1 mentions)
Glycine, by Sisco Research Laboratories (1 mentions)
Nicotinamide adenine dinucleotide, by Sisco Research Laboratories (1 mentions)
Czapek dox medium, by Sisco Research Laboratories (1 mentions)
Bisacrylamide, by Sisco Research Laboratories (1 mentions)
3 protocols using dimethyl sulphoxide
1
Fractionation and Evaluation of Methanol Extract
2
Curcumin-NDEA Interaction Study Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Curcumin and N-nitrosodiethylamine were purchased from Sigma Chemicals Co. (USA). Triton X, ethidium bromide, agarose (normal and low-melting), dimethylsulphoxide, Tris, EDTA, and all other chemicals were purchased from SISCO Research Laboratories, India. May Grünwald stain and Giemsa stain were procured from Merck Ltd. (India).
3
Isolation and Characterization of Penicillium chrysogenum SNP5
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Penicilliumchrysogenum SNP5 (MTCC13144) strain was locally isolated from grease-contaminated soil of the diesel loco shed and identified by Microbial Type Culture Collection and gene bank Chandigarh, India23 (link). Triton X-100, Phenylmethylsulfonyl fluoride (PMSF), Lauryl dimethylamine oxide (LDAO), Nicotinamide adenine dinucleotide (NAD) + hydrogen (NADH), 1, 6- diphenyl- 1, 3, 5- hexatriene (DPH), Acrylamide, Bis Acrylamide, Coomassie brilliant blue R-250, Glycine, Bromophenol blue, Methanol, para-nitrophenol, 1-Bromooctane, Dimethyl Sulphoxide (DMSO), Petroleum ether, diethyl ether, other medium components, and Czapek-dox medium were procured from Sisco Research Laboratories Pvt. Ltd. (Mumbai, India). Hexadecane and THB (Tetrahydrobiopterin), Acetic acid, and Digitonin were procured from TCI Chemicals Pvt. Ltd. (India). Phosphatidylcholine and cholesterol were purchased from Sigma-Aldrich Chemicals Pvt. Ltd. PageRuler™ Plus Prestained Protein Ladder was procured from Thermo Scientific.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!