DOPC and DSPE-(PEG)2000-OMe were purchased from Avanti Polar Lipids and Nanosoft Polymers, respectively. Cholesterol was purchased from Sigma. Everolimus and EG00229 were obtained from LC laboratories and Tocris Bioscience, respectively. Ki67 (ab15880), PD-L1 (PA5-88105), β-actin (A2228), and YM1 (60130) antibodies were purchased from Abcam, Invitrogen, Sigma, and STEMCELL Technologies, respectively. The goat-anti-mouse PD-1 antibody was a kind gift from Dr. Keith L. Knutson (Mayo Clinic).
Dspe peg 2000 ome
Manufactured by Avanti Polar Lipids
Sourced in United States
DSPE-(PEG)2000-OMe is a lipid molecule composed of distearoylphosphatidylethanolamine (DSPE) conjugated to a polyethylene glycol (PEG) chain of approximately 2000 molecular weight, with a methoxy group at the distal end of the PEG chain. This compound is commonly used as a steric stabilizer and solubilizing agent in the formulation of liposomes and other lipid-based drug delivery systems.
Automatically generated - may contain errors
Lab products found in correlation
Cholesterol, by Merck Group (3 mentions)
Everolimus, by LC Laboratories (3 mentions)
Phospho p70s6k, by Cell Signaling Technology (2 mentions)
Antibodies against mtor, by Cell Signaling Technology (1 mentions)
Sunitinib, by MedChemExpress (1 mentions)
Ym155, by MedChemExpress (1 mentions)
Parp1, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Cabozantinib, by LC Laboratories (1 mentions)
Calf thymus dna, by Merck Group (1 mentions)
Cholesterol, by Chron Chemicals (1 mentions)
1 2 dioleoyl sn glycero 3 phosphoethanolamine n carboxyfluorescein cfpe, by Avanti Polar Lipids (1 mentions)
Scrambled sirna, by GenePharma (1 mentions)
Bcl 2 sirna, by GenePharma (1 mentions)
Cy5 labeled sirna, by GenePharma (1 mentions)
4 protocols using dspe peg 2000 ome
1
Liposomes Loaded with Everolimus
2
Nanomedicine Delivery Protocol
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DOPC and DSPE-(PEG)2000-OMe were purchased from Avanti Polar Lipids and Nanosoft Polymers, respectively. Cholesterol was purchased from Sigma. TTP-conjugated lipopeptide was synthesized as described previously. Everolimus and YM155 were obtained from LC laboratories and MedChemExpress, respectively. Antibodies against mTOR, phospho-mTOR, p70S6K, phospho-p70S6K, survivin, ATM, PARP1, and β-actin were obtained from Cell Signaling Technology. ATR, CHk1, and Chk2 antibodies were obtained from Santa Cruz Biotechnology. CD3, CD8, and Ki67 antibodies were from Abcam, while CD45 antibody was from Biolegend.
3
Liposome Formulation for Cancer Therapy
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DOPC and DSPE-PEG(2000)-OMe were purchased from Avanti Polar Lipids and Nanosoft Polymers, respectively. Cholesterol was purchased from Sigma. TTP-conjugated lipopeptide was synthesized as described previously [40] . Everolimus and cabozantinib were obtained from LC laboratories. YM155 and sunitinib were obtained from MedChemExpress and Selleckchem, respectively. Antibodies against mTOR, phospho-mTOR, p70S6K, phospho-p70S6K, survivin, ATM, PARP1, CD4, and β-actin were obtained from Cell Signaling Technology. ATR, Chk1, and Chk2 antibodies were obtained from Santa Cruz Biotechnology. CD3, CD8, and Ki67 antibodies were from Abcam, while CD45 antibody was from Biolegend. The phospho-p70S6K antibody used for immunohistochemistry was from Invitrogen. Purified anti-PD-1 antibody [42] was a kind gift from Dr. Keith L. Knutson (Mayo Clinic) who obtained it from the Antibody Core Facility at Mayo Clinic (Rochester, MN).
4
Lipid-Based Nanoparticle Delivery System for siRNA Therapy
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Cholesterol was purchased from Chengdu Kelong Chemical Company (Chengdu, China). Soybean phospholipids (SPC) were purchased from Shanghai Taiwei Chemical Company (Shanghai, China). 1, 2-dioleoyl-3-trimethylammonium-propane (DOTAP) was obtained from Shanghai Advanced Vehicle Technology (AVT) Ltd. (Shanghai, China). DSPE-PEG2000-OMe and 1, 2-dioleoyl-sn-glycero-3- phosp-hoethanolamine-N-(carboxyfluorescein) (CFPE ) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Protamine sulfate (fraction X from salmon) and calf thymus DNA were purchased from Sigma-Aldrich (St. Louis, MO, USA). Paclitaxel (PTX) and low-molecular-weight heparin (Enoxaparin sodium, LH) were purchased from Melonepharma (Dalian, China). BCL-2 siRNA, scrambled siRNA and Cy5-labeled siRNA were purchased from Gene Pharma Ltd. (Shanghai, China). The BCL-2 siRNA (siBCL-2) sense sequence is 5'-GUG AUG AAG UAC AUC CAU UdTdT-3', and the anti-sense sequence is 5'-AAU GGA UGU ACU UCA UCA CdTdT-3'. The scramble siRNA (siCTL) sense sequence is 5'-UUC UCC GAA CGU GUC ACG UTT-3', and the anti-sense sequence is 5'-ACG UGA CAC GUU CGG AGA ATT-3'.
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