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Bz x700 inverted microscope

Manufactured by Keyence
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Sourced in Japan

The BZ-X700 is an inverted microscope designed for laboratory use. It features a motorized stage and autofocus functionality, allowing for automated imaging and sample observation. The BZ-X700 supports various imaging techniques, including brightfield, phase contrast, and fluorescence microscopy.

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Lab products found in correlation

Prolong gold antifade with dapi, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

BZ-X700 (661 citations) BZ-X700 microscope (366 citations) Inverted microscope (9 citations) BZ-X700 inverted fluorescence microscope (9 citations) BZ-X700 All-in-One inverted fluorescence microscope (4 citations)

3 protocols using bz x700 inverted microscope

1

Immunofluorescent Staining of Cav1 and Filaggrin

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Following TOF-SIMS analysis, samples were fixed in ice cold acetone for 2 min and air dried prior to staining. Samples were blocked for 1hr with 2.5% normal goat serum in Phosphate Buffered Saline supplemented with 0.01% Tween (PBSt), prior to incubating with Rabbit anti-Cav1 (1:100, Sigma Aldrich, St. Louis, MO, USA, cat # HPA049326) and mouse anti-filaggrin antibodies (1:200 Abcam, Cambridge, UK, cat# ab3137) overnight at 4oC and then with either Alexa 488 anti-rabbit or Alexa 594 anti-mouse secondary antibodies (Life technologies, Carlsbad, CA, USA) and mounted using Prolong Gold Antifade with DAPI (Life Technologies). Immunofluorescent and phase contrast images were obtained using a Keyence BZ-X700 inverted microscope (Osaka, Japan) and compared to TOF-SIMS-based imaging.
Castellanos A., Hernandez M.G., Tomic-Canic M., Jozic I, & Fernandez-Lima F. (2019). Multimodal, in-situ imaging of ex-vivo human skin reveals decrease of cholesterol sulfate in the neoepithelium during acute wound healing. Analytical chemistry, 92(1), 1386-1394.
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2

Microscopic Imaging and Analysis

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Slide and organoid imaging were performed using a Keyence BZ-X700 Inverted Microscope. Scale bars were added using ImageJ Software (NIH).
Bader C.S., Barreras H., Lightbourn C.O., Copsel S.N., Wolf D., Meng J., Ahn J., Komanduri K.V., Blazar B.R., Jin L., Barber G.N., Roy S, & Levy R.B. (2020). STING differentially regulates experimental GVHD mediated by CD8 versus CD4 T cell subsets. Science translational medicine, 12(552), eaay5006.
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3

Quantitative Analysis of GUS Staining

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GUS staining was performed as previously described (Notaguchi, Wolf, & Lucas, 2012), and sections of stained root and stem tissue were hand sectioned using a razor blade. Root whole mounts were used for examination of lateral root primordia (Péret et al., 2009). Light microscopy of GUS‐stained tissue was carried out using a Keyence BZ‐X700 inverted microscope. Quantitative GUS activity assays (Figure 1d) were carried out on five‐day‐old seedlings grown on either standard Somerville and Ogren medium (9 mM
NO3-) or a nitrogen‐free version of the same medium (KCl replaces KNO3 and CaCl2 replaces Ca(NO3)2) (Patterson et al., 2010). Total protein extraction and the 4‐methylumbelliferyl β‐D‐glucuronide (MUG) assay were performed as previously described (Jefferson, 1987).
Ehrary A., Rosas M., Carpinelli S., Davalos O., Cowling C., Fernandez F, & Escobar M. (2020). Glutaredoxin AtGRXS8 represses transcriptional and developmental responses to nitrate in Arabidopsis thaliana roots. Plant Direct, 4(6), e00227.
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