Sgc 7901

Manufactured by Shanghai Institute of Biochemistry and Cell Biology

Sourced in China

The SGC-7901 is a laboratory cell line derived from a human gastric adenocarcinoma. It is commonly used in cell biology and cancer research applications.

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25 protocols using sgc 7901

H. pylori Infection of Gastric Cells

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HEK293T cells and human GAC cell line AGS was obtained from ATCC. Immortalized normal human gastric epithelial cell line GES-1 and human GAC cell lines BGC-823 and SGC-7901 were obtained from Shanghai Institute of Cell Biology, China Academy of Sciences (Shanghai, China). HEK293T cells were cultured in DMEM medium, and other cells were cultured in RPMI-1640 medium supplemented with 10% FBS at 37°C in 5% CO₂ in a 25-cm² flask.
The wild-type H. pylori strains 26695 were obtained from ATCC. The bacteria were grown on CDC anaerobe blood agar plates (BD) and were incubated at 37°C for 2 days in an anaerobic jar containing a gas mixture of 5% O₂, 10% CO₂, and 85% N₂ (DU Scientific).
When the cells reached 80% confluency, the medium was replaced with antibiotic-free medium and H. pylori was added to the flask at a multiplicity of infection of 100:1. At 18 h after H. pylori induction, the cells were lysed to extract total RNA.

Liu W., Song N., Yao H., Zhao L., Liu H, & Li G. (2015). miR-221 and miR-222 Simultaneously Target RECK and Regulate Growth and Invasion of Gastric Cancer Cells. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 21, 2718-2725.

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Culturing Human Gastric Cell Lines

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Human gastric cancer cell line SGC-7901, cisplatin resistant cell line SGC-7901/DDP and normal gastric epithelial cell line GES-1 were purchased from the Shanghai Institute of Cell Biology (Shanghai, China). Cells were cultured in PRIM-1640 (GIBCO, Rockville, USA) supplemented with 10% fetal bovine serum (GIBCO, Rockville, USA) and 1% antibiotics and grown at 37 °C in humidified air with 5% CO 2 .

Yan J., Zhang Y., She Q., Li X., Peng L., Wang X., Liu S., Shen X., Zhang W., Dong Y., Lu J, & Zhang G. (2017). Long Noncoding RNA H19/miR-675 Axis Promotes Gastric Cancer via FADD/Caspase 8/Caspase 3 Signaling Pathway. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 42(6).

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Culturing Immortalized Gastric Cell Lines

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The immortalized normal gastric mucosal epithelial cell line (GES-1) and human GC cell lines MKN-45, BGC-823, SGC-7901, MKN-28, and AGS were purchased from Shanghai Institute of Cell Biology (Shanghai, China). Cells were cultured in RPMI 1640 (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and supplemented with 10% fetal bovine serum (FBS) at 37 °C in a humidified atmosphere with 5% CO₂.

Li W., Li J., Mu H., Guo M, & Deng H. (2019). MiR-503 suppresses cell proliferation and invasion of gastric cancer by targeting HMGA2 and inactivating WNT signaling pathway. Cancer Cell International, 19, 164.

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Gastric Cancer Cell Line Irradiation

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Three human gastric adenocarcinoma cell lines (BGC823, SGC7901, and MKN45) and an immortalized human gastric mucosa cell line (GES-1) were purchased from the Shanghai Institute of Cell Biology (Shanghai, People’s Republic of China) and maintained in Roswell Park Memorial Institute 1640 medium with 10% calf bovine serum, 50 units/mL penicillin, and 50 units/mL streptomycin in 5% CO₂ at 37°C. Radiotherapy was administered in vitro using a 4 MeV electron beam linear accelerator (Elekta, Stockholm, Sweden).

Cui F.B., Liu Q., Li R.T., Shen J., Wu P.Y., Yu L.X., Hu W.J., Wu F.L., Jiang C.P., Yue G.F., Qian X.P., Jiang X.Q, & Liu B.R. (2014). Enhancement of radiotherapy efficacy by miR-200c-loaded gelatinase-stimuli PEG-Pep-PCL nanoparticles in gastric cancer cells. International Journal of Nanomedicine, 9, 2345-2358.

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Cultivation of Gastric Cancer Cell Lines

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Human poorly differentiated BGC823 and moderately differentiated SGC7901 gastric cancer cell lines were obtained from Shanghai Institute of Cell Biology (Shanghai, China). All cells were routinely cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. All the cells were kept at 37 °C in a humidified atmosphere of 5% CO₂.

Sun M.X., He X.P., Huang P.Y., Qi Q., Sun W.H., Liu G.S, & Hua J. (2020). Acetyl-11-keto-β-boswellic acid inhibits proliferation and induces apoptosis of gastric cancer cells through the phosphatase and tensin homolog /Akt/ cyclooxygenase-2 signaling pathway. World Journal of Gastroenterology, 26(38), 5822-5835.

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Cell Culture and Gastric Cancer Samples

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BGC-823, SGC-7901 and HEK-293 T cell lines were purchased from Shanghai Institute of Cell Biology (Shanghai, China). BGC-823 and SGC-7901 cells were cultured in RPMI-1640 medium (Gibco, USA) while HEK-293 T cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco). All medium was added with 10% fetal bovine serum (Gibco), 100 U/mL benzyl penicillin, and 100 μg/mL streptomycin. All cell lines were incubated at 37 ℃ in a humidified atmosphere containing 5% CO₂. The paraffin-embedded slides of gastric cancer were provided by Nanjing Drum Tower Hospital. The protocol was approved by The Institutional Ethical Committee of Nanjing Drum Tower Hospital.

Ding Y., Xu Y., Fu Y., Zhang H., Zhao L, & Fan X. (2022). Kruppel-like factor 13 inhibits cell proliferation of gastric cancer by inducing autophagic degradation of β-catenin. Discover. Oncology, 13, 121.

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Gastric Cancer Cell Line and Xenograft Model Protocol

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Ptx and Tet were purchased from Sigma-Aldrich (St Louis, MO). Type-B gelatin (225 bloom strength), with 100–115 mmol of carboxylic acid per 100 g of protein, an isoelectric point of 4.7–5.2, and an average molecular weight of 40–50 kDa, was purchased from Sigma-Aldrich (St Louis, MO). Polyethylene glycol (PEG) at a molecular weight of 4 kd was purchased from Sigma-Aldrich (St Louis, MO). ε-Caprolactone (ε-CL, Sigma) was dehydrated by CaH₂ at room temperature and distilled under reduced pressure. 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan (MTT) was purchased from Sigma-Aldrich. All remaining reagents were of analytical grade and used without further purification.
The human low-differentiated gastric adenocarcinoma cell line, BGC-823 and SGC-7901, was purchased from the Shanghai Institute of Cell Biology (Shanghai, China) and cultured in RPMI 1640 medium with 10% fetal bovine serum and 100 U/ml penicillin-streptomycin at 37 °C in a humidified atmosphere with 5% CO₂.
Six to eight-week-old BALB/c athymic nude mice were purchased from the Shanghai SLAC Laboratory Animal Co., Ltd. (Shanghai, China). The mice were housed and maintained in the animal facility of the Animal Center of Nanjing Medical University. The animal protocol was reviewed and approved by the Animal Care and Use Committee of Nanjing Medical University.

Zhang H., Tian Y., Zhu Z., Xu H., Li X., Zheng D, & Sun W. (2016). Efficient antitumor effect of co-drug-loaded nanoparticles with gelatin hydrogel by local implantation. Scientific Reports, 6, 26546.

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Cell Culture of Gastric Cancer Lines

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Human gastric adenocarcinoma AGS cells (ATCC, Manassas, VA, USA) were grown in F-12k (ATCC). Other GC cells MKN-45, BGC-823, SGC-7901, MKN-28 and normal human gastric epithelial cell line GES-1 (Shanghai Institute of Cell Biology, China) were grown in RPMI-1640 (Gibco). All of these cells were supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin at 37°C with humidified 5% CO₂.

Liu F., Cao Q.H., Lu D.J., Luo B., Lu X.F., Luo R.C, & Wang X.G. (2015). TMEM16A overexpression contributes to tumor invasion and poor prognosis of human gastric cancer through TGF-β signaling. Oncotarget, 6(13), 11585-11599.

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Culturing Human Gastric Cell Lines

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The human GC cell lines (BGC823, MGC803, SGC7901, MKN45, AGS, and N87) and gastric immortalized epithelial cell line (GES1) were purchased from the Shanghai Institute of Cell Biology. All cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco BRL, Grand Island, NY) supplemented with 10% heat-inactivated fetal bovine serum (FBS) containing 100 units/mL penicillin and 100 mg/mL streptomycin (P/S, Gibco) at 37°C in a 5% CO₂ incubator.

Dong Z., Guo S., Wang Y., Zhang J., Luo H., Zheng G., Yang D., Zhang T., Yan L., Song L., Liu K., Sun Z., Meng X., Zheng Z., Zhang J, & Zhao Y. (2020). USP19 Enhances MMP2/MMP9-Mediated Tumorigenesis in Gastric Cancer. OncoTargets and therapy, 13, 8495-8510.

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Establishing 5Fu-Resistant GC Cell Lines

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Both GC cell lines, SGC7901 and AGS, were purchased from the Shanghai Institute of Cell Biology (Shanghai, China) and stored at our institute. The cell lines were maintained under the recommended culture conditions and incubated at 37°C in a humidified environment containing 5% CO₂. Prior to 5Fu exposure, 1 × 10⁶ cells were seeded into 10-cm-diameter dishes and cultured for 24 h. The cultures were then maintained in increasing 5Fu concentrations (AGS: 5, 7.5, 10, 20, and 30 μg/mL; SGC790115: 30, 45, and 60 μg/mL; Nantong Jinghua Pharmaceutical Ltd. Co., Jiangsu, China) for 24 h per passage. This process was continued for approximately 3 months.

Xu Z.Y., Tang J.N., Xie H.X., Du Y.A., Huang L., Yu P.F, & Cheng X.D. (2015). 5-Fluorouracil Chemotherapy of Gastric Cancer Generates Residual Cells with Properties of Cancer Stem Cells. International Journal of Biological Sciences, 11(3), 284-294.

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