BeWo cell lines were donated by Jiayin Liu Laboratory of Nanjing Medical University and cultured according to the supplier's instructions. The cells were maintained at standard culture conditions of 5% CO 2 in air at 37˚C. Cells were grown in an adherent condition in Ham's F12 medium (Sigma-Aldrich, St Louis, Mo, USA) containing 10% fetal bovine serum (FBS, Culti-lab), 100 U/mL penicillin and 100 μg/mL streptomycin. The cells were seeded into 24-well plates at 1× 10 4 cells/well to form a con uent monolayer, and then switched to the medium without FBS to induce differentiation. When the cells reached to 70% -80% con uence, different concentrations (0 μg/mL, 50 μg/mL, 100 μg/mL, 200 μg/mL, 400 μg/mL) of AGEs-BSA were added to the medium for various times (6 h, 12 h and 24 h). Besides, anti-RAGE (10 μg/mL, Abcam, Cambridge, UK ) or ammonium pyrrolidinedithiocarbamate (PDTC, 100 μM/mL, Beyotime Co. Ltd, Shanghai, China) were added to the medium for 2 h before treatment with optimal concentration of AGEs-BSA.
Ammonium pyrrolidinedithiocarbamate pdtc
Manufactured by Beyotime
Sourced in United Kingdom
Ammonium pyrrolidinedithiocarbamate (PDTC) is a chemical compound commonly used in analytical chemistry and biochemistry. It functions as a chelating agent, forming stable complexes with various metal ions. PDTC is primarily utilized for the detection, separation, and quantification of trace metals in various samples.
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Lab products found in correlation
3 protocols using ammonium pyrrolidinedithiocarbamate pdtc
1
Modulating BeWo cell differentiation
2
MDCK Cell Culture for H9N2 Influenza
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The MDCK cells (ATCC, CCL-34) were cultivated in a T-flask in Dulbecco’s Modified Eagle Medium (DMEM) (BioEngine Sci-Tech, Shanghai, China) supplemented with 10% (v/v) fetal bovine serum (FBS) (Biosun, Shanghai, China) at 37 °C in a humidified incubator with 5% CO2. Influenza A virus (A/chicken/Guangxi/SIC6/2013(H9N2)) was generously provided by Guangdong Wens Dahuanong Biotechnology Co., LTD (Yunfu, China). The ammonium pyrrolidinedithiocarbamate (PDTC) was from Beyotime Biotechnology (Shanghai, China).
3
Modulating BeWo cell differentiation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BeWo cell lines were donated by Jiayin Liu Laboratory of Nanjing Medical University and cultured according to the supplier's instructions. The cells were maintained at standard culture conditions of 5% CO 2 in air at 37˚C. Cells were grown in an adherent condition in Ham's F12 medium (Sigma-Aldrich, St Louis, Mo, USA) containing 10% fetal bovine serum (FBS, Culti-lab), 100 U/mL penicillin and 100 μg/mL streptomycin. The cells were seeded into 24-well plates at 1× 10 4 cells/well to form a con uent monolayer, and then switched to the medium without FBS to induce differentiation. When the cells reached to 70% -80% con uence, different concentrations (0 μg/mL, 50 μg/mL, 100 μg/mL, 200 μg/mL, 400 μg/mL) of AGEs-BSA were added to the medium for various times (6 h, 12 h and 24 h). Besides, anti-RAGE (10 μg/mL, Abcam, Cambridge, UK ) or ammonium pyrrolidinedithiocarbamate (PDTC, 100 μM/mL, Beyotime Co. Ltd, Shanghai, China) were added to the medium for 2 h before treatment with optimal concentration of AGEs-BSA.
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