Cd38 apc clone hb7

Manufactured by BD

CD38-APC (clone HB7) is a fluorochrome-conjugated monoclonal antibody that binds to the CD38 antigen. CD38 is a transmembrane glycoprotein expressed on the surface of various cell types, including lymphocytes, monocytes, and plasma cells. This product can be used for the identification and enumeration of CD38-positive cells in flow cytometry applications.

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Close spelling variants of this product

CD38-APC (42 citations) CD38 (HB7) (4 citations) CD38 (clone HB7; (2 citations) APC; HB7 (2 citations)

2 protocols using cd38 apc clone hb7

Cytotoxicity of DDA in AML Cells

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The cytotoxic response to DDA of AML cells was determined by flow cytometry. Cells were exposed to DDA for 24 or 48 h then washed with cold phosphate-buffered saline (PBS) and resuspended in 200 µl of Annexin-V binding buffer. For the analysis of AML patient samples, cells were incubated with antibodies against the surface markers CD45-V450 (clone H130), CD34-PeCy7 (clone 8G12), CD38-APC (clone HB7) and CD123-PE (clone 9F5) (BD Pharmagen) before being resuspended in Annexin-V binding buffer. Annexin-V-fluorescein isothiocyanate (BD Pharmagen, clone 2331) and 7-aminoactinomycin D (7AAD) were then added, and the samples were incubated in the dark at room temperature for 15 min. The percentage of viable cells, Annexin-V-7AAD-negative cells, was scored using a flow cytometer.

Segala G., David M., de Medina P., Poirot M.C., Serhan N., Vergez F., Mougel A., Saland E., Carayon K., Leignadier J., Caron N., Voisin M., Cherier J., Ligat L., Lopez F., Noguer E., Rives A., Payré B., Saati T.A., Lamaziere A., Despres G., Lobaccaro J.M., Baron S., Demur C., de Toni F., Larrue C., Boutzen H., Thomas F., Sarry J.E., Tosolini M., Picard D., Record M., Récher C., Poirot M, & Silvente-Poirot S. (2017). Dendrogenin A drives LXR to trigger lethal autophagy in cancers. Nature Communications, 8, 1903.

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Fluorochrome-Conjugated Antibody Panel

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Fluorochrome-conjugated monoclonal antibodies (mAbs) used included FITC-Mouse-IgG1 (clone X40), PE-Mouse-IgG1 (clone MOPC-21), APC-Mouse-IgG1 (clone SJ25C1), PerCP-Mouse-IgG1 (clone X40), APC-CD25 (clone 2A3), PerCP-CD4 (clone SK3), FITC-CD8 (clone 2D1), CD19-APC (clone SJ25C1), HLA-DR (clone L243), CD38-APC (clone HB7), CD3-FITC (clone SK7), CD16 + 56-PE (clone B73.1), CD45-PerCP (clone 2D1), CD4-FITC (clone SK3), CD8-PE (clone SK1) (BD Biosciences, San Jose, CA).

Lu H., Zhang L., Dai Y., Ruan Y., Cao X., Cai X., Ruan S, & Chen Q. (2020). Markers of immune activation: novel biomarkers to predict the early-warning indicator of patients with papillary thyroid carcinoma. Diagnostic Pathology, 15, 16.

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