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Sprout mini centrifuge

Manufactured by Biozym
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Sourced in Germany

The Sprout mini centrifuge is a compact and portable laboratory equipment designed for basic separation and sample preparation tasks. It has a maximum speed of 6,000 RPM and a capacity of up to 2 x 2 mL microtubes. The centrifuge is suitable for a variety of applications that require rapid sedimentation of small sample volumes.

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Lab products found in correlation

μ dishes, by Ibidi (2 mentions) A4018, by Merck Group (2 mentions) Plan fluor 20x air objective, by Nikon (2 mentions) Halolink resin, by Promega (1 mentions)

3 protocols using sprout mini centrifuge

1

Immobilization of Glutamate Sensor

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Immobilization of the Glu[+Halo] sensor was performed at room temperature. Before use, the Halo-Link® resin (particle size = 45–165 µm, Promega, Mannheim, Germany) was washed twice with MOPS buffer (20 mM, pH 7.3). A suspension of the resin (100 µL) was incubated with 1 mL Glu[+Halo] solution (20 µM) for 1 h in a 1.5 mL Eppendorf tube under constant slow inversion. After centrifugation in a tabletop centrifuge (10 s, 2000×g, Sprout Minicentrifuge, Biozym, Hessisch Oldendorf, Germany) the supernatant was removed. Afterwards the resin was washed twice with 20 mM MOPS buffer. The immobilized Glu[+Halo] sensor was stored at 4 °C suspended in MOPS buffer (20% v/v) in the dark. Loading of the beads with the sensor was estimated by comparing the absorption of Venus (λex = 515 nm, ε = 92,200 M−1 cm−1) of the sensor solution before and after immobilization.
Otten J., Tenhaef N., Jansen R.P., Döbber J., Jungbluth L., Noack S., Oldiges M., Wiechert W, & Pohl M. (2019). A FRET-based biosensor for the quantification of glucose in culture supernatants of mL scale microbial cultivations. Microbial Cell Factories, 18, 143.
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2

Live Imaging of Infected Larvae in Agarose

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For live imaging of infected larvae, chambers were prepared by placing two 2.5 mm x 5 mm strips of non-toxic double-sided tape (TES5338, Tesa, Norderstedt, Germany) at the periphery of 35 mm μ-Dishes (# 81166, Ibidi, Gräfelfing, Germany). 1.5% low gelling agarose (LGA) (#A4018, Sigma-Aldrich) was dissolved in FASW by heating to 80 °C until liquid. The liquid LGA was cooled to and kept at 37 °C until use. 300 larvae in a volume of 1 ml were placed in a 1.5 ml Eppendorf centrifuge tube and quickly vortexed using a Sprout mini centrifuge (#552021, Biozym Scientific GmbH, Hessisch Oldendorf, Germany) to pellet larvae. The larvae and 1.5% LGA at 37 °C were mixed for a final LGA concentration of 1.14% LGA. The mixture was pipetted into the center of the prepared imaging chamber and a glass coverslip was pressed on top of the droplet to adhere on either end to the double-sided tape. The Ibidi plate was filled with 2 ml of FASW. Microscopic analysis was conducted with a Nikon Eclipse Ti inverted microscope using a Nikon Plan Fluor 20x air objective, with images taken every 5 or 15 minutes (as indicated) in DIC and TexasRed channels. Images were processed with Fiji software 87 (link).
Jacobovitz M.R., Rupp S., Voss P.A., Maegele I., Gornik S.G, & Guse A. (2021). Dinoflagellate symbionts escape vomocytosis by host cell immune suppression. Nature microbiology, 6(6), 769-782.
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3

Live Imaging of Infected Larvae in Agarose

Check if the same lab product or an alternative is used in the 5 most similar protocols
For live imaging of infected larvae, chambers were prepared by placing two 2.5 mm x 5 mm strips of non-toxic double-sided tape (TES5338, Tesa, Norderstedt, Germany) at the periphery of 35 mm μ-Dishes (# 81166, Ibidi, Gräfelfing, Germany). 1.5% low gelling agarose (LGA) (#A4018, Sigma-Aldrich) was dissolved in FASW by heating to 80 °C until liquid. The liquid LGA was cooled to and kept at 37 °C until use. 300 larvae in a volume of 1 ml were placed in a 1.5 ml Eppendorf centrifuge tube and quickly vortexed using a Sprout mini centrifuge (#552021, Biozym Scientific GmbH, Hessisch Oldendorf, Germany) to pellet larvae. The larvae and 1.5% LGA at 37 °C were mixed for a final LGA concentration of 1.14% LGA. The mixture was pipetted into the center of the prepared imaging chamber and a glass coverslip was pressed on top of the droplet to adhere on either end to the double-sided tape. The Ibidi plate was filled with 2 ml of FASW. Microscopic analysis was conducted with a Nikon Eclipse Ti inverted microscope using a Nikon Plan Fluor 20x air objective, with images taken every 5 or 15 minutes (as indicated) in DIC and TexasRed channels. Images were processed with Fiji software 87 (link).
Jacobovitz M.R., Rupp S., Voss P.A., Maegele I., Gornik S.G, & Guse A. (2021). Dinoflagellate symbionts escape vomocytosis by host cell immune suppression. Nature microbiology, 6(6), 769-782.
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