Antibodies, chemicals, and plasmids used in this study are listed in Supplemental Table S1. HEK293T cells (RBC2202; RIKEN BioResource Research Center) were cultured in DMEM with high glucose (Nacalai Tesque) supplemented with 5% fetal bovine serum (FBS). hTERT-RPE1 cells (CRL-4000; American Type Culture Collection) were grown in DMEM/Ham’s F-12 medium (Nacalai Tesque) supplemented with 10% FBS and 0.348% sodium bicarbonate at 37°C in 5% CO2. IFT27-KO cells (cell line #IFT27-2-2) and CEP19-KO cells (cell lines #CEP19-1-2 and #CEP19-1-12) were established from hTERT-RPE1 cells, as described previously (Nishijima et al., 2017 (link); Zhou et al., 2022 (link)).
Htert rpe1 cells crl 4000
Manufactured by American Type Culture Collection
HTERT-RPE1 cells (CRL-4000) are a human retinal pigment epithelial cell line that has been immortalized through the ectopic expression of the human telomerase reverse transcriptase (hTERT) gene. These cells retain many of the characteristics of normal retinal pigment epithelial cells.
Automatically generated - may contain errors
2 protocols using htert rpe1 cells crl 4000
1
Establishment of IFT27 and CEP19 Knockout Cell Lines
2
Engineered RPE-1 hTERT TP53-/- Cell Line
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RPE-1 hTERT Cas9 TP53−/− (female human hTERT-immortalized retinal pigmented epithelial cells) was constructed as previously described23 (link). hTERT RPE-1 cells (CRL-4000) were obtained from the American Type Culture Collection (ATCC) and were grown in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM:F12) supplemented with 10% FBS and 1% penicillin–streptomycin. HAP1 cells were obtained from Horizon Discovery and maintained in Iscove’s Modified Dulbecco’s Medium supplemented with 10% FBS and 1% penicillin/streptomycin. Cells were grown at 37 °C and 5% CO2 in standard tissue culture incubators. Cells were regularly tested for mycoplasma contamination with the PCR-based Venora GeM Mycoplasma Detection Kit; no mycoplasma contamination was detected during this study.
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