Mice were fasted for 6 h and given either an oral gavage of glucose (2 g/kg body weight) or an intraperitoneal injection of glucose (1 g/kg body weight). Tail blood was collected and blood glucose measured with HemoCue 201+ analyzer (HemoCue, Ängelholm, Sweden) before (30 and 0 min) and after (15, 30, 60, 90 and 120 min) gavage or injection. Tail blood was also collected with Microvette CB 300 Z (Sarstedt, Nümbrecht-Rommelsdorf, Germany) for serum insulin analysis using the Ultra-Sensitive Mouse Insulin ELISA kit (Crystal Chem, Downers Grove, IL, USA) according to the manufacturer's protocol.
201 analyzer
Manufactured by HemoCue
Sourced in Sweden
The HemoCue 201+ analyzer is a portable device designed to perform hemoglobin measurements. It utilizes a photometric method to analyze a small blood sample and provide accurate results.
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Lab products found in correlation
4 protocols using 201 analyzer
1
Glucose Tolerance and Insulin Response
2
Measurement of Hemoglobin, Insulin, and Iron
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Hemoglobin was measured with a HemoCue201+ analyzer (HemoCue). Plasma insulin levels were measured by ELISA (Crystal Chem Inc.), plasma lactate and alanine by kit (Sigma-Aldrich), and liver total iron (nonheme and heme) levels by inductively coupled plasma mass spectroscopy conducted by the diagnostic center at Michigan State University (15 ,16 (link)).
3
Parasitemia Monitoring in Malaria-Infected Mice
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Control and immunized mice were challenged 4 weeks after the last CII with 1 × 105P. chabaudi AS or P. yoelii YM for homologous or heterologous experiments. Age-matched naive mice were challenged alongside the experimental groups to account for residual drug. Parasitemia was monitored every alternate day by the use of Giemsa-stained blood films, and the hemoglobin level was measured every 4 days (HemoCue 201+ analyzer; Hemocue, Angelholm, Sweden). Percent parasitemia was calculated as the number of infected RBCs divided by total RBC count (a minimum of 300 total RBCs were counted) multiplied by 100. To assess health, mice received clinical scores every 2 days (76 (link)). Mice that showed signs of severe disease were euthanized using CO2 gas. Observer bias was reduced through experimental blinding, except for experiments requiring immune cell depletion.
4
In Vivo Gene Delivery and Induction
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C57BL6 mice were maintained under a 12-h:12-h light:dark cycle in a clean facility with free access to food and water. Experiments were performed with the approval of the Animal Ethics Committee (DEC) in the Netherlands. Three-month-old animals were intravenously injected with AAV5; EPO-expressing viruses at 1e−12 and IGF-expressing viruses at 1e−13 vector genome copies per animal. Four weeks later, mice injected with AAV5-GS-IGF were treated with 20 mg/kg MFP 3 days in a row, except for the non-induced group of mice. In the experiment concerning EPO expression, 4 and 8 weeks post-transduction, several groups of mice were injected intraperitoneally with 20 mg/kg MFP for 4 consecutive days. Blood was taken on the indicated days of the experiment; in general, before transduction and before and after induction with MFP. All blood samples were collected in tubes with heparin, and after centrifugation at 1,500 rpm for 15 min, plasma was stored at −80°C and used for ELISA or MFP measurement at a later stage. Hematocrit was analyzed using a HemoCue 201+ analyzer (HemoCue, Ängelholm, Sweden), with a drop of blood immediately collected on a microcuvette. When the animals were sacrificed, their livers were dissected.
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