Baf401

Manufactured by R&D Systems

BAF401 is a high-purity recombinant protein that can be used as a laboratory reagent. It is a member of the Bromodomain and Extra-Terminal (BET) family of proteins.

Automatically generated - may contain errors

Lab products found in correlation

Mab401, by R&D Systems (2 mentions) Mab453, by R&D Systems (1 mentions) Mab275, by R&D Systems (1 mentions) Baf453, by R&D Systems (1 mentions) Ebio17ck15a5, by Thermo Fisher Scientific (1 mentions) Baf275, by R&D Systems (1 mentions) Quantikine elisa kit, by R&D Systems (1 mentions) Luminex multiplex assay, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Nigericin, by Thermo Fisher Scientific (1 mentions) Ly6g fitc, by BD (1 mentions) Uric acid, by Merck Group (1 mentions) Celltiter blue kit, by Promega (1 mentions) Crid3, by Bio-Techne (1 mentions) Anti cd45 bv421, by BD (1 mentions) Vx 765, by Selleck Chemicals (1 mentions) Ly6c percp, by BD (1 mentions) Lps ultra pure, by InvivoGen (1 mentions) Duoset, by R&D Systems (1 mentions)

3 protocols using baf401

Cytokine Profiling of Respiratory Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

Culture supernatants and BAL samples were assayed for IL-1β, MIP-1α, TNFα, IL-6, IL-10, IL-18, IL-17A and CXCL1 by ELISA. Antibody pairs for the IL-1β (MAB401 and BAF401; clone 30311 and polyclonal; R&D Systems; 8 and 4 μg ml⁻¹, respectively) and CXCL1 (mouse: MAB453 and BAF453; clone 48415 and polyclonal; R&D Systems; 8 and 0.4 μg ml⁻¹, respectively; human: MAB275, clone 20326, 4 μg ml⁻¹; BAF275, polyclonal, 40 ng ml⁻¹) and CCL3/ MIP-1α (Quantikine ELISA kit, Product #MMA00) ELISAs were from R&D Systems. Antibody pairs for IL-1α (capture: product #14-7011-85, clone ALF-161; detection: product #13-7111-85, polyclonal), TNFα (capture: product #14-7325-85, clone 1F3F3D4; detection: product #13-7326-85, clones MP6-XT22 and MP6-XT3), IL-6 (capture: product #14-7061-85, clone MP5-20F3; detection: product #13-7062-85, clone MP5-32C11), IL-10 (capture: product #14-7101-85, clone JES5-16E3; detection: product #13-7102-85, clone JES5-2A5) and IL-17A (capture: product #14-7175-85, clone eBio 17CK15A5; detection: product #13-7177-85, clone eBio17B7) were from eBiosciences and used at a 1:250 dilution. Antibody pairs for IL-18 were from MBL International (capture: product #D047-3, clone 74, 1:1,000; detection: product #D048-6, clone 93-10C, 1:2,000).

Ulland T.K., Jain N., Hornick E.E., Elliott E.I., Clay G.M., Sadler J.J., Mills K.A., Janowski A.M., Volk A.P., Wang K., Legge K.L., Gakhar L., Bourdi M., Ferguson P.J., Wilson M.E., Cassel S.L, & Sutterwala F.S. (2016). Nlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment. Nature Communications, 7, 13180.

+ Open protocol

+ Expand

Cytokine Profiling of Leishmania-Infected Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cell suspensions were generated from draining retromaxillary lymph nodes or spleens from L. major infected or control mice by passage through a sterile 70 µM screen. Red blood cells in splenic preparations were lysed by hypotonic shock. The cells were then seeded at 2×10⁵ cells/well in 200 µl of RP10 [RPMI 1640 (Gibco), 10% heat-inactivated FBS (Gibco), 2 mM L-glutamine, 50 µg/ml gentamicin, in round bottomed 96-well plates with or without stimuli. The stimuli included media control, 10 µg/ml total Leishmania lysate, live L. major parasites at 3:1 parasite: host cell ratio, or wells coated with anti-CD3e antibody at 500 ng/mL overnight. After incubation at 37°, 5% CO₂ for 72 hours, supernatants were collected for cytokine assays. The murine cytokines IFN-γ, IL-1β, IL-4, IL-12p70, IL-6, IL-17A, TNFα, and IL-10 were analyzed in cell supernatants by a Multiplex Luminex Assay (Life Technologies) or Bio-Plex Multiplex Assay (Millipore). IL-17A was detected in whole ear lysates by ELISA using the mouse IL-17A DuoSet ELISA kit from R&D. IL-1β was detected in by ELISA using monoclonal coating and detection antibodies MAB401 and BAF401, respectively, from R&D.

Clay G.M., Valadares D.G., Graff J.W., Ulland T.K., Davis R.E., Scorza B.M., Zhanbolat B.S., Chen Y., Sutterwala F.S, & Wilson M.E. (2017). An anti-inflammatory role for NLRP10 in murine cutaneous leishmaniasis. Journal of immunology (Baltimore, Md. : 1950), 199(8), 2823-2833.

+ Open protocol

+ Expand

Inflammasome Activation and Cytokine Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell culture reagents (e.g., PBS, DMEM, RPMI, Trypsin‐EDTA) were from Gibco, Thermo Fisher Scientific. Stimuli were as follow: LPS (UltraPure, Invivogen), nigericin (Thermo Fisher Scientific), PFO (Biotrend), LFn‐BsaK, LFn‐MxiH and PA were produced in‐house as described previously (19), and uric acid used for in‐house production of MSU crystals (Sigma‐Aldrich). Inhibitors used were CRID3 (Tocris) and VX‐765 (Selleckchem). Antibodies were as follow: anti‐ASC polyclonal antibody (AL177, Adipogen, 50 µg ml⁻¹) or anti‐ASC monoclonal antibody (N‐15, Santa Cruz, 1:500), anti‐Caspase‐1 p20 antibody (Casper‐1, Adipogen, 1:1,000), anti‐IL‐1β antibody (BAF401, R&D Systems, 1:1,000), and anti‐GSDMD (L60, Cell Signaling Technology, 1:1,000). DRAQ5 was from Thermo Fisher Scientific. For FACS, Abs used were anti‐CD45‐BV421, Ly6G‐FITC and Ly6C‐PerCP (BD Biosciences, 1:100). HTRF for human or mouse IL‐1β were from CisBio. ELISA kits were used to detect IL‐1β, TNFα, IL‐6 or CXCL1 (DuoSet, R&D Systems). Cell viability was measured with the Cell Titer Blue kit (CTB, Promega).

Bertheloot D., Wanderley C.W., Schneider A.H., Schiffelers L.D., Wuerth J.D., Tödtmann J.M., Maasewerd S., Hawwari I., Duthie F., Rohland C., Ribeiro L.S., Jenster L., Rosero N., Tesfamariam Y.M., Cunha F.Q., Schmidt F.I, & Franklin B.S. (2022). Nanobodies dismantle post‐pyroptotic ASC specks and counteract inflammation in vivo. EMBO Molecular Medicine, 14(6), e15415.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!