PET imaging of tumor-bearing mice was performed on a microPET R4 rodent model scanner (Siemens Medical Solutions USA, Inc., Malvern, Pennsylvania). The mice bearing A431 tumors (for each group n=4) were injected with Al18F-NOTA-ZEGFR:1907 (1.9-2.6 MBq) or 18F-CBT-ZEGFR:1907 (1.48-2.22 MBq) spiked with 30 or 300 μg of non-radioactive Ac-Cys-ZEGFR:1907 or Cys-ZEGFR:1907 through the tail vein. At 1, 2 and 3 h after injection, the mice were anesthetized with 2% isoflurane and placed near the center of the field of view of the microPET scanner in prone position. Three-minute static scans were obtained, and the images were reconstructed by a two-dimensional ordered subsets expectation maximum (OSEM) algorithm. No background correction was performed. Regions of interest (ROIs; 5 pixels for coronal and transaxial slices) were drawn over the tumors on decay-corrected whole-body coronal images. The maximum counts per pixel per minute were obtained from the ROIs and converted to counts per milliliter per minute using a calibration constant. Tissue density was assumed to be 1 g/mL, and the ROIs were converted to counts per gram per minute. Image ROI-derived %ID/g values were determined by dividing counts per gram per minute by the injected dose. No attenuation correction was performed.
Micropet r4 rodent model scanner
Manufactured by Siemens
Sourced in United States
The MicroPET R4 is a rodent model scanner designed for small animal imaging. It is a high-resolution positron emission tomography (PET) system capable of imaging small laboratory animals such as mice and rats. The MicroPET R4 allows for the non-invasive, in vivo visualization and quantification of biological processes at the molecular and cellular level.
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Lab products found in correlation
4 protocols using micropet r4 rodent model scanner
1
In Vivo PET Imaging of EGFR in Tumor-Bearing Mice
2
Lung Tumor Xenograft Imaging with 64Cu Probes
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All animal experiments
were performed under a protocol approved by the University of Southern
California Institutional Animal Care and Use Committee (IACUC). To
establish a lung tumor xenograft model, 2 × 106 of
A549 or NCI-H249 cells were subcutaneously injected in the right shoulder
of nude mice as previous reported.22 ,23 (link)The
tumor-bearing mice were injected with 3.7–7.4 MBq of 64Cu probes via tail veins. For each probe, 3 randomly selected mice
were used. Multiple static scans were obtained at 3, 16, 28, and 45
h postinjection (p.i.). PET imaging and analysis were conducted by
using a Siemens microPET R4 rodent model scanner as described previously.23 (link),25 (link)
were performed under a protocol approved by the University of Southern
California Institutional Animal Care and Use Committee (IACUC). To
establish a lung tumor xenograft model, 2 × 106 of
A549 or NCI-H249 cells were subcutaneously injected in the right shoulder
of nude mice as previous reported.22 ,23 (link)The
tumor-bearing mice were injected with 3.7–7.4 MBq of 64Cu probes via tail veins. For each probe, 3 randomly selected mice
were used. Multiple static scans were obtained at 3, 16, 28, and 45
h postinjection (p.i.). PET imaging and analysis were conducted by
using a Siemens microPET R4 rodent model scanner as described previously.23 (link),25 (link)
3
Radiolabeled Integrin Targeting Probe
4
PET Imaging of EGFR-Expressing Tumors in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PET imaging of tumor-bearing mice was performed on a microPET R4
rodent model scanner (Siemens Medical Solutions USA, Inc., Malvern,
Pennsylvania). The mice bearing A431 tumors (for each group n = 4) were injected with Al18F-NOTA-ZEGFR:1907 (1.9–2.6 MBq) or 18F-CBT-ZEGFR:1907 (1.48–2.22 MBq) spiked with 30 or 300 μg of nonradioactive
Ac-Cys-ZEGFR:1907 or Cys-ZEGFR:1907 through
the tail vein. At 1, 2, and 3 h after injection, the mice were anesthetized
with 2% isoflurane and placed near the center of the field of view
of the microPET scanner in prone position. Three-minute static scans
were obtained, and the images were reconstructed by a two-dimensional
ordered subsets expectation maximum (OSEM) algorithm. No background
correction was performed. Regions of interest (ROIs; 5 pixels for
coronal and transaxial slices) were drawn over the tumors on decay-corrected
whole-body coronal images. The maximum counts per pixel per minute
were obtained from the ROIs and converted to counts per milliliter
per minute using a calibration constant. Tissue density was assumed
to be 1 g/mL, and the ROIs were converted to counts per gram per minute.
Image ROI-derived %ID/g values were determined by dividing counts
per gram per minute by the injected dose. No attenuation correction
was performed.
rodent model scanner (Siemens Medical Solutions USA, Inc., Malvern,
Pennsylvania). The mice bearing A431 tumors (for each group n = 4) were injected with Al18F-NOTA-ZEGFR:1907 (1.9–2.6 MBq) or 18F-CBT-ZEGFR:1907 (1.48–2.22 MBq) spiked with 30 or 300 μg of nonradioactive
Ac-Cys-ZEGFR:1907 or Cys-ZEGFR:1907 through
the tail vein. At 1, 2, and 3 h after injection, the mice were anesthetized
with 2% isoflurane and placed near the center of the field of view
of the microPET scanner in prone position. Three-minute static scans
were obtained, and the images were reconstructed by a two-dimensional
ordered subsets expectation maximum (OSEM) algorithm. No background
correction was performed. Regions of interest (ROIs; 5 pixels for
coronal and transaxial slices) were drawn over the tumors on decay-corrected
whole-body coronal images. The maximum counts per pixel per minute
were obtained from the ROIs and converted to counts per milliliter
per minute using a calibration constant. Tissue density was assumed
to be 1 g/mL, and the ROIs were converted to counts per gram per minute.
Image ROI-derived %ID/g values were determined by dividing counts
per gram per minute by the injected dose. No attenuation correction
was performed.
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