D510 80 wide band emission filter

For lysosomal calcium measurements, we performed Oregon Green^® 488 BAPTA-1 dextran (OGBD) labeling^{41 (link)}. In this assay, cells with a stable transduction of LAMP1-mRFP lentiviruses were loaded with 0.1 mg ml⁻¹ OGBD (Invitrogen) for 30 min. For cytosolic calcium measurements, we performed X-Rhod-1 and Fura-2 labeling assays. In these assays, cells were loaded with 1 μM X-Rhod-1-AM (Invitrogen) or 1 μM Fura-2-AM (Invitrogen) for 30 min. For OGBD and X-Rhod-1 imaging, the cells were examined under a temperature- and CO₂-controlled laser confocal microscope (Olympus); for ratiometric Fura-2 imaging, cells were examined under a temperature- and CO₂-controlled fluorescence microscope equipped with excitation wavelengths of 340 and 380 nm (Till Polychrome V, Till Photonics, Gräfelfing, Germany), a 410-dLcp beamsplitter, a D510/80 wide band emission filter (Chroma Technology Corp., Rockingham, VT, USA) and a Quant-EM camera (Photometrics, Tucson, AZ, USA).