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Phosphorylated pdk1

Manufactured by Cell Signaling Technology
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Phosphorylated PDK1 is a laboratory product that can be used to detect and quantify the level of phosphorylated PDK1 (3-Phosphoinositide-dependent protein kinase 1) in biological samples. PDK1 is a serine/threonine protein kinase that plays a crucial role in the activation of various signaling pathways, including the PI3K/Akt pathway. The phosphorylated form of PDK1 is an important marker for the activation of these pathways.

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Lab products found in correlation

Phosphorylated akt, by Cell Signaling Technology (2 mentions) Phosphorylated p70s6k, by Cell Signaling Technology (1 mentions) Proliferating cell nuclear antigen (pcna), by Cell Signaling Technology (1 mentions) Cleaved poly adp ribose polymerase, by Cell Signaling Technology (1 mentions) Phosphorylated gsk3β, by Cell Signaling Technology (1 mentions) Phosphorylated 4ebp1, by Cell Signaling Technology (1 mentions) Foxo1, by Cell Signaling Technology (1 mentions) Total akt, by Cell Signaling Technology (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) Nf κb p65, by Cell Signaling Technology (1 mentions) Estradiol e2, by Merck Group (1 mentions) Total eif2α, by Cell Signaling Technology (1 mentions) Total stat3, by Cell Signaling Technology (1 mentions) Z ietd fmk, by Abcam (1 mentions) Phosphorylated stat3, by Cell Signaling Technology (1 mentions) Nfκbp105, by Cell Signaling Technology (1 mentions) Total pdk1, by Cell Signaling Technology (1 mentions) Phosphorylated eif2α, by Cell Signaling Technology (1 mentions) Erα sc 544, by Santa Cruz Biotechnology (1 mentions) Caspase 7, by Cell Signaling Technology (1 mentions)

2 protocols using phosphorylated pdk1

1

Antibody Immunoblotting of Signaling Proteins

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Antibodies against Akt, phosphorylated Akt, PI3K, phosphorylated PDK1, phosphorylated TSC2, phosphorylated GSK3β, phosphorylated p70S6K, phosphorylated 4EBP1, mTOR, PTEN, FOXO1, PCNA, and cleaved poly ADP ribose polymerase (C-PARP) were from Cell Signaling Technology (Danvers, MA). Antibodies against glyceraldehyde 3-phosphate dehydrogenase (GAPDH), survivin, CDK4, and pRb were from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies against phosphorylated mTOR and phosphorylated PTEN were from Abcam (Cambridge, UK). Antibody against Cyclin D1 was from Biosource (Camarillo, CA).
Choi A.R., Kim J.H, & Yoon S. (2014). Sensitization of Cancer Cells through Reduction of Total Akt and Downregulation of Salinomycin-Induced pAkt, pGSk3β, pTSC2, and p4EBP1 by Cotreatment with MK-2206. BioMed Research International, 2014, 295760.
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2

Investigating Cellular Stress Pathways

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Estradiol (E2) and dexamethasone (Dex) were purchased from Sigma-Aldrich. The caspase-8 inhibitor Z-IETD-FMK was bought from BioVision. Recombinant human TNFα was obtained from Biomyx. For Western blotting, antibodies against total eIF2α, phosphorylated eIF2α, IRE-1α, total Akt, phosphorylated Akt, total PDK1, phosphorylated PDK1, total STAT3, phosphorylated STAT3, NF-κB p65, NF-κB p105, cleaved PARP, and caspase-7 were obtained from Cell Signaling Technology. ERα (sc-544) and GR (sc-8992) were purchased from Santa Cruz Biotechnology.
Fan P., Siwak D.R., Abderrahman B., Agboke F.A., Yerrum S, & Jordan V.C. (2019). Suppression of Nuclear Factor-κB by Glucocorticoid Receptor Blocks Estrogen-induced Apoptosis in Estrogen-deprived Breast Cancer Cells. Molecular cancer therapeutics, 18(10), 1684-1695.
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