Rinm5f β cells

Manufactured by American Type Culture Collection

Sourced in United States

RINm5F β-cells are a pancreatic beta cell line derived from a radiation-induced rat insulinoma. These cells are capable of insulin secretion and are commonly used in research related to pancreatic beta cell function and diabetes.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) 1640 medium, by Thermo Fisher Scientific (1 mentions) Hepes, by Greiner (1 mentions) Glutamax supplement, by Thermo Fisher Scientific (1 mentions) Tissue culture flask, by Greiner (1 mentions)

2 protocols using rinm5f β cells

Tat-CIAPIN1 Protein Transduction in RINm5F Cells

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Pancreatic β-cells (RINm5F β-cells) were obtained from the ATCC (Manassas, VA, USA) and maintained in RPMI1640 medium containing 10% FBS and 1% antibiotics.
To assess the ability of Tat-CIAPIN1 protein transduction, RINm5F β-cells were treated with 0.5-3 μM or 15-60 min of Tat-CIAPIN1 protein in culture medium. After washing, transduced levels were determined by Western blotting.

Yeo H.J., Shin M.J., Kim D.W., Kwon H.Y., Eum W.S, & Choi S.Y. (2021). Tat-CIAPIN1 protein prevents against cytokine-induced cytotoxicity in pancreatic RINm5F β-cells. BMB Reports, 54(9), 458-463.

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Culturing Insulinoma RINm5F β-Cells

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All cell culture reagents were purchased from Sigma-Aldrich (Dorset, England) unless otherwise specified. Pancreatic insulinoma RINm5F β-cells (ATCC, CRL11605, Rockville, MD, USA) from Rattus norvegicus were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (32404, Gibco, Loughborough, England) supplemented with 10% (v/v) fetal bovine serum (F9665), 1 mM sodium pyruvate (11360070, Gibco, Loughborough, England), 4500 mg/L glucose (G8644), 2 mM Glutamax supplement (35050061, Gibco, Loughborough, England), 20 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and maintained at 37 °C, 5% CO₂, and 95% relative humidity in tissue culture flasks (Greiner Bio-One, Kremsmünster, Austria). Cultures were routinely split (1:2) at ~80% confluency by rinsing the cells with Dulbecco’s phosphate buffered saline (DPBS, 10 mm) without calcium or magnesium and released for sub-cultivation using 0.25% (v/v) trypsin-EDTA.

Bongiovanni M.N., Aprile F.A., Sormanni P, & Vendruscolo M. (2018). A Rationally Designed Hsp70 Variant Rescues the Aggregation-Associated Toxicity of Human IAPP in Cultured Pancreatic Islet β-Cells. International Journal of Molecular Sciences, 19(5), 1443.

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