Milli q advantage 10 a water system

The polyclonal anti-albumin antibodies (goat, fractionated human antiserum, product A1151), Protein G-Sepharose 4B fast flow (recombinant protein expressed in E. coli), immunoglobulin G (goat, ≥ 95% pure) and HSA (essentially fatty acid free, ≥ 96%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The racemic warfarin (≥ 98%), acetohexamide (99%), gliclazide (≥ 98%), glipizide (˃ 96%), glibenclamide (≥ 99%), and tolbutamide (99.8%) were also acquired from Sigma-Aldrich. The chlorpropamide (≥ 99%) and tolazamide (≥ 99%) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The Nucleosil Si-300 and Si-1000 silica (7 μm, particle diameter; pore size, 300 or 1000 Å, respectively) were acquired from Macherey-Nagel (Duren, Germany). All buffers and aqueous solutions were prepared using water from a Milli-Q Advantage 10 A Water system and were filtered using 0.2 μm nylon membranes (EMD Millipore Corporation, Billerica, MA, USA).

The polyclonal anti-HSA antibodies (goat, affinity-purified polyclonal antibodies, product 2080–01) were purchased from VWR (Radnor, PA, USA). The polyclonal anti-HSA goat serum (product A1151), protein G Sepharose 4B fast flow (recombinant protein expressed in E. coli), immunoglobulin G (IgG; goat, ≥ 95% pure), HSA (essentially fatty acid free, ≥ 96%), glycogen (from bovine liver, ≥ 85% glucose), oxalic dihydrazide (> 99%), acetohexamide (99%), glibenclamide (≥ 99%), and racemic warfarin (≥ 98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The Nucleosil Si-300 and Si-1000 silica (7 µm, particle diameter; pore size, 300 or 1000 Å, respectively) were acquired from Macherey-Nagel (Duren, Germany). All buffers and aqueous solutions were prepared using water from a Milli-Q Advantage 10 A Water system and were filtered using 0.2 µm nylon membranes (EMD Millipore Corporation, Billerica, MA, USA).