Mouse anti ace2

Cells were seeded in 8-well chamber slides (Thermo) and fixated with 3–4% formaldehyde for 15–30 min at room temperature. To detect HCV infection, cells were immunostained with human serum derived from HCV-infected patient, followed by incubation with anti-human Cy3 (Jackson) secondary antibody. To detect SARS-CoV-2 infection, the fixed cells were blocked with PBS containing 2% FBS and stained with hyperimmune rabbit serum from intravenous SARS-CoV-2-infected rabbits (in-house preparation), and then with Alexa Fluor 488-conjugated goat anti-rabbit IgG (Sigma). To detect ACE2 expression, cells were immunostained with mouse anti-ACE2 (Abcam) or goat anti-ACE2 (R &D systems), and then with anti-mouse 488 Alexa fluor IgG (Jackson) and anti-goat IgG (Invitrogen), respectively, and counterstained with DAPI. Images were obtained with a Zen Live Imaging (Time Lapse) microscope (Zeiss) or confocal microscope (LSM 780 + Chameleon Vision II).

Cells were seeded in 8-well chamber slides (Thermo) and fixated with 3–4% formaldehyde for 15–30 min at room temperature. To detect HCV infection, cells were immunostained with human serum derived from HCV-infected patient, followed by incubation with anti-human Cy3 (Jackson) secondary antibody. To detect SARS-CoV-2 infection, the fixed cells were blocked with PBS containing 2% FBS and stained with hyperimmune rabbit serum from intravenous SARS-CoV-2-infected rabbits (in-house preparation), and then with Alexa Fluor 488-conjugated goat anti-rabbit IgG (Sigma). To detect ACE2 expression, cells were immunostained with mouse anti-ACE2 (Abcam) or goat anti-ACE2 (R &D systems), and then with anti-mouse 488 Alexa fluor IgG (Jackson) and anti-goat IgG (Invitrogen), respectively, and counterstained with DAPI. Images were obtained with a Zen Live Imaging (Time Lapse) microscope (Zeiss) or confocal microscope (LSM 780 + Chameleon Vision II).