Alizarin red staining kit

Manufactured by IHC World

The Alizarin Red staining kit is a laboratory tool used for the detection and visualization of calcium deposits in biological samples. It provides a simple and effective method for staining calcium-containing structures, such as calcified tissues or mineralized bone, for analysis and research purposes.

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Lab products found in correlation

Alcian blue, by IHC World (1 mentions) Bh l microscope, by Olympus (1 mentions)

2 protocols using alizarin red staining kit

Multilineage Differentiation Potential of cAT-MSCs

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To confirm the cAT-MSC mesodermal differentiation ability, the media for adipogenesis, chondrogenesis, and osteogenesis induction was replaced over 2–3 days, and differentiation was induced over 21 days (Table 1). The differentiated cells were washed twice with PBS, fixed with 4% paraformaldehyde for 10 min, and then rewashed with PBS. To evaluate adipogenic differentiation, the fixed cells were stained with Oil Red O staining kit (IHC World, Ellicott City, MD, United States), with red lipid vacuoles accumulating in the differentiated cells. After chondrogenic differentiation, the cells were fixed and stained with Alcian Blue (IHC World) to detect the presence of glycosaminoglycans. Following osteogenic differentiation, the presence of extracellular calcium was confirmed using an Alizarin Red staining kit (IHC World).

Kang S.J., Gu N.Y., Byeon J.S., Hyun B.H., Lee J, & Yang D.K. (2023). Immunomodulatory effects of canine mesenchymal stem cells in an experimental atopic dermatitis model. Frontiers in Veterinary Science, 10, 1201382.

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Quantifying Cardiac Fibrosis and Calcification

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Hearts were perfused and excised from isoflurane-euthanized mice, washed in cold PBS. The upper and middle part of the hearts were fixed in 4% paraformaldehyde, embedded in paraffin and sectioned at 5 μm thickness. Collagen was quantified by masson’s trichrome staining as we described previously [38 (link)]. The blue staining represented collagen deposition. A series of 4 sections of each mouse (4 mice per group) was examined and photographed using an Olympus BH-L microscope coupled with a digital color camera. Using ImageJ software, blue-stained areas and non-stained myocyte areas from each section were determined using color-based thresholding. The percentage of total fibrosis area was calculated as the summed blue stained areas divided by total ventricular areas.
Cardiac calcium deposition was analyzed by using alizarin red staining kit (IHC WORLD, Woodstock, MD) as per the manufacturer’s protocol. Calcification area was quantified with Image J as described above.

Chen K., Wang S, & Sun Z. (2022). In Vivo Cardiac-specific Expression of Adenylyl Cyclase 4 Gene Protects against Klotho Deficiency-induced Heart Failure. Translational research : the journal of laboratory and clinical medicine, 244, 101-113.

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