For measurement of cytokine production by CD4+ T cells in culture, supernatants were collected at 48 hr or 5 days and stored at −20° celsius until analysis. Cytokines were measured using LEGENDplex Human Th Cytokine Panel (12-plex) (BioLegend, Cat# 741028), and IFN-γ levels measured by enzyme-linked immunosorbent assay (ELISA) using anti-IFN-γ capture (Bio-Rad, Cat# HCA043) and biotinylated detection (Bio-Rad, Cat# HCA044P) anti-bodies, recombinant IFN-γ standard (Bio-Rad, Cat# PHP050), streptavidin-HRP (Sigma-Aldrich, Cat# E2866), and TMB substrate (Bio-Rad, Cat# BUF062B).
Anti ifn γ capture
Manufactured by Bio-Rad
Anti-IFN-γ capture is a laboratory equipment product designed to detect and measure the presence of interferon-gamma (IFN-γ) in samples. It functions as a capture antibody, binding to IFN-γ molecules and enabling their identification and quantification through subsequent detection methods.
Automatically generated - may contain errors
2 protocols using anti ifn γ capture
1
Cytokine Profiling of CD4+ T Cells
2
Cytokine Profiling of CD4+ T Cells
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For measurement of cytokine production by CD4+ T cells in culture, supernatants were collected at 48 hr or 5 days and stored at −20° celsius until analysis. Cytokines were measured using LEGENDplex Human Th Cytokine Panel (12-plex) (BioLegend, Cat# 741028), and IFN-γ levels measured by enzyme-linked immunosorbent assay (ELISA) using anti-IFN-γ capture (Bio-Rad, Cat# HCA043) and biotinylated detection (Bio-Rad, Cat# HCA044P) anti-bodies, recombinant IFN-γ standard (Bio-Rad, Cat# PHP050), streptavidin-HRP (Sigma-Aldrich, Cat# E2866), and TMB substrate (Bio-Rad, Cat# BUF062B).
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