All samples were randomized throughout the multiwell plates, and laboratory personnel were blinded to case status. IgG was isolated from individual plasma samples using 96-well protein G monolithic plates, eluted with 0.1 mol/L formic acid, and neutralized with 1 mol/L ammonium bicarbonate as previously described in detail (27 ). Prepared samples were stored at −20°C until ultraperformance liquid chromatography analysis on a Waters ACQUITY UPLC H-Class instrument (27 ). All chromatograms were separated in the same manner into 24 IgG-GPs, and the amount of glycans in each peak was expressed as the percentage of the total integrated area (Supplementary Fig. 3 and Supplementary Table 1 ). The Supplementary Methods provide a more detailed description of IgG glycoprofiling.
Plasma adiponectin was measured with a commercially available sandwich ELISA (LINCO Research). HDL and total cholesterol, triglycerides, hemoglobin A1c (HbA1c), and hs-CRP were measured using an automatic ADIVA 1650 analyzer (Siemens Medical Solutions) at the University of Tübingen.
Plasma adiponectin was measured with a commercially available sandwich ELISA (LINCO Research). HDL and total cholesterol, triglycerides, hemoglobin A1c (HbA1c), and hs-CRP were measured using an automatic ADIVA 1650 analyzer (Siemens Medical Solutions) at the University of Tübingen.