QDs and Homer1C-GFP signals were detected by using a mercury lamp (for QDs: excitation filter 560RDF55 or 460BP40 and emission filters 655WB20 or 655WB40). Fluorescent images from QDs were obtained with an integration time of50 mswith upto 1,200 consecutiveframes. Signalswere recorded with a back-illuminated thinned CCD97 camera (Photometrics Cascade 512B; Roper Scientific).
Photometrics cascade 512b
Manufactured by Teledyne
The Photometrics Cascade 512B is a scientific-grade digital camera designed for low-light imaging applications. It features a high-resolution 512 x 512 pixel sensor with a 16-bit analog-to-digital converter, enabling the capture of detailed images with high dynamic range. The camera provides a fast frame rate and is optimized for sensitive detection of faint signals.
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Lab products found in correlation
4 protocols using photometrics cascade 512b
1
Quantifying Fluorescent Protein Signals
2
Time-lapse Imaging of Particle Deposition
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A specific volume of a dispersion of the MPs in the medium of desired concentration, was placed on a fibronectin-coated glass coverslip fitted in a magnetic imaging chamber (Chamlide CMB, CM-B25-1) filled with CO2-equilibrated culture medium. After a given time, ranging from few minutes for silica particles and after a few hours for polystyrene particles, aggregates were deposited at random on the coated glass coverslip. The final volume of cultured medium was 1.5 mL. The chamber was sealed with mineral oil to prevent water evaporation and placed for viewing in an inverted microscope (TIRF AF 6000LX, Leica) equipped with a ×10 0.30 NA objective. Videos were recorded with a CCD camera (Photometrics Cascade 512B, Roper Scientific) at an acquisition rate of 1 frame every 10 min. Images were exported from the instrument software in TIFF format and visualized using the ImageJ software package v.1.46r (National Institutes of Health, Bethesda, MD).
3
Time-lapse Imaging of Cellular Aggregates
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The polymer-coated coverslips were placed
on the bottom of a microscope magnetic observation chamber (Chamlide
CMB, CM-B25-1). Droplets containing cellular aggregates were removed
from the Petri dish lid with a pipet and placed on the polymer-coated
glass coverslips. The microscope cell was filled with CO2-equilibrated culture medium, consisting of DMEM supplemented with
10% (v/v) FBS and antibiotics (100 μg mL–1 streptomycin and 100 U mL–1 penicillin) to reach
a final volume of 1.5 mL. The chamber was sealed with mineral oil
to prevent water evaporation and placed for viewing in an inverted
microscope (TIRF AF 6000LX, Leica) equipped with a 10× 0.30 NA
objective. Videos were recorded with a CCD camera (Photometrics Cascade
512B, Roper Scientific) at an acquisition rate of 1 frame every 10
min, exported from the instrument software in TIFF format, and visualized
using the ImageJ software package v.1.46r (National Institutes of
Health, Bethesda, MD) at 37 °C.
on the bottom of a microscope magnetic observation chamber (Chamlide
CMB, CM-B25-1). Droplets containing cellular aggregates were removed
from the Petri dish lid with a pipet and placed on the polymer-coated
glass coverslips. The microscope cell was filled with CO2-equilibrated culture medium, consisting of DMEM supplemented with
10% (v/v) FBS and antibiotics (100 μg mL–1 streptomycin and 100 U mL–1 penicillin) to reach
a final volume of 1.5 mL. The chamber was sealed with mineral oil
to prevent water evaporation and placed for viewing in an inverted
microscope (TIRF AF 6000LX, Leica) equipped with a 10× 0.30 NA
objective. Videos were recorded with a CCD camera (Photometrics Cascade
512B, Roper Scientific) at an acquisition rate of 1 frame every 10
min, exported from the instrument software in TIFF format, and visualized
using the ImageJ software package v.1.46r (National Institutes of
Health, Bethesda, MD) at 37 °C.
4
Imaging Sodium Dynamics in mTAL Cells
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The mTAL strips attached to cover slips were incubated with HBSS-H containing 10 nM of the Na+-sensitive fluorescence dye Sodium Green (Na Green) and 0.05% Pluronic F-127 for 30 min at room temperature (RT). After 3 washes with HBSS-H to remove an excess of Na Green, cover slip was placed to the heated chamber mounted on the stage of an inverted microscope. The number given for each experimental measurement corresponds to a separate rat in all cases. Na Green fluorescence images were obtained using a Nikon TE-2000U inverted microscope equipped with a 60/1.1 water immersion objective lens and a high-resolution digital camera (Photometrics Cascade 512B, Roper Scientific, Tucson, AZ). Excitation was provided by a 175-W xenon arc lamp (model DG-4, Sutter Instrument, Novato, CA) at alternating wavelengths, and emission was controlled using an optical filter changer (Lambda 10-3, Sutter Instrument)26 (link). Na Green was excited at 480/40 nm, and emission signal from 522/30 nm was acquired every 10 s. The signals were normalized by subtraction of the first time point value from each data point.
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