Sw 13

Manufactured by American Type Culture Collection

Sourced in United States

The SW-13 is a type of laboratory equipment designed for cell culture applications. It functions as a water bath, providing a controlled temperature environment for maintaining and incubating cell cultures.

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Lab products found in correlation

9 protocols using sw 13

Comprehensive Cell Line Database

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All cell lines were of human origin. Melanoma cell lines DOR, Beu, and Hbl were previously described [29 (link)]. Other melanoma cell lines (MeWo, SK-MEL-2, SK-MEL-28, SK-MEL-5, SK-MEL-3, Malme 3M, HT144, WM35, WM1552C, and RPMI-7931) were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA). Normal human melanocytes HeMN-LP were from Cascade Biologics (Portland, OR, USA). NSCLC lung cancer cell lines A549, HT1299, A-427, Calu-1, H-460, H-520, H596, H-661, H-2170, and SK-MES-1, and SCLC cell lines H-446, H-69, H-209, H-82, H-345, H-146, H-378, H-196 were purchased from ATCC. 293FT cells were from Invitrogen (Carlsbad, CA, USA). Colorectal cell lines LoVo, SW480, HCT116 were from ATCC. All other cell lines were purchased also from ATCC: G-401 and A-204 (rhabdoid tumors), U-2 OS and Saos-2 (osteosarcomas), HeLa S3 and C33A (cervical carcinomas), 293 (renal carcinoma), HT-1080 (connective tissue fibrosarcoma), SW-13 (adrenal gland carcinoma), T98G (glioblastoma), IMR90 and WI-38 (normal human fibroblasts), Jurkat (T-cell leukemia), Hep-G2 (hepatocellular carcinoma), SK-N-SH and SH-N-MC (neuroblastomas), PANC-1, PA-TU-8902, MIA PaCa-2, and BxPC-3 (pancreatic carcinomas).

Réda J., Vachtenheim J., Vlčková K., Horák P., Vachtenheim J J.r, & Ondrušová L. (2018). Widespread Expression of Hedgehog Pathway Components in a Large Panel of Human Tumor Cells and Inhibition of Tumor Growth by GANT61: Implications for Cancer Therapy. International Journal of Molecular Sciences, 19(9), 2682.

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Culturing Adrenocortical Carcinoma Cell Lines

Cited 1 time

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NCI-H295R (RRID: CVCL_0458) and SW-13 (RRID: CVCL_0542) were purchased from ATCC. CU-ACC1 (RRID: CVCL_RQ00) and CU-ACC2 (RRID: CVCL_RQ01) were provided by Dr. K. Kiseljak-Vassiliades (5 (link)). NCI-H295R cells were grown in 1:1 DMEM:F12 (Thermo Fisher Scientific) supplemented with 2.5% Nu-Serum (Corning), 1% ITS media supplement (R&D Systems), and 1% Penicillin–Streptomycin (Thermo Fisher Scientific). SW13 was grown in DMEM (Thermo Fisher Scientific) supplemented with 10% FBS (GeminiBio) and 1% Penicillin–Streptomycin (Gibco). CU-ACC1 and CU-ACC2 ACC cell lines were grown in F medium [3:1 (v/v) Ham's F-12 Nutrient Mixture–DMEM (Thermo Fisher Scientific) supplemented with 5% FBS, 0.4 µg/mL hydrocortisone (Sigma-Aldrich), 5 µg/mL insulin (Sigma-Aldrich), 8.4 ng/mL cholera toxin (Sigma-Aldrich), 10 ng/mL EGF (Invitrogen), 24 µg/mL adenine (Sigma-Aldrich)] (5 (link)). All cell lines were cultured at 37°C in a humidified incubator with 5% CO₂. Cell line identification tests were performed by short tandem repeat polymorphism analysis, using previously reported standard methods (5, 26 (link)). All cell lines were tested by MycoAlert Mycoplasma Detection Kit (Lonza) and confirmed to be Mycoplasma negative.

Arakawa Y., Jo U., Kumar S., Sun N.Y., Elloumi F., Thomas A., Roper N., Varghese D.G., Takebe N., Zhang X., Ceribelli M., Holland D.O., Beck E., Itkin Z., McKnight C., Wilson K.M., Travers J., Klumpp-Thomas C., Thomas C.J., Hoang C.D., Hernandez J.M., Del Rivero J, & Pommier Y. (2024). Activity of the Ubiquitin-activating Enzyme Inhibitor TAK-243 in Adrenocortical Carcinoma Cell Lines, Patient-derived Organoids, and Murine Xenografts. Cancer Research Communications, 4(3), 834-848.

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Culturing ACC Cell Lines for Research

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Human ACC cell lines H295R (high cortisol secretor) and SW13 (nonsecretor) were obtained from ATCC. RL251 (nonsecretor) was derived using a clinical protocol approved by the Institutional Review Board at the University of Michigan Hospital and validated by the provider.31 (link) H295R cells were grown in 1:1 DDMEM:F12 nutrient mixture (Thermo Fisher Scientific) supplemented with 5% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and ITS (final concentrations 0.001 mg/mL bovine insulin, 0.0055 mg/mL human transferrin, and 6.7 ng/mL sodium selenite; Thermo Fisher Scientific). ACC cell lines SW13 and RL251 were grown in DMEM supplemented with 10% FBS. All cell lines were cultured at 37°C in a humidified incubator with 5% CO₂.

Kuai R., Subramanian C., White P.T., Timmermann B.N., Moon J.J., Cohen M.S, & Schwendeman A. (2017). Synthetic high-density lipoprotein nanodisks for targeted withalongolide delivery to adrenocortical carcinoma. International Journal of Nanomedicine, 12, 6581-6594.

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Cell Culture Protocols for VSMC, HASMC, and MEF

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Rat VSMCs (A10, ATCC) and human adrenal carcinoma cells (SW13, ATCC) were maintained in Dulbecco's modified Eagle's medium (Invitrogen) supplemented with 10% fetal bovine serum (Hyclone). Human primary aortic smooth muscle cells (HASMC) were maintained in specialized medium supplied by the vendor (Lifeline Cell Technology, Frederick, MD, USA). Mouse embryonic fibroblast (MEF) cells were isolated from wild type (WT) or MRTF-A knockout mice (22 (link)). Recombinant endothelin-1 (ET-1) was purchased from Sigma.

Yang Y., Cheng X., Tian W., Zhou B., Wu X., Xu H., Fang F., Fang M, & Xu Y. (2014). MRTF-A steers an epigenetic complex to activate endothelin-induced pro-inflammatory transcription in vascular smooth muscle cells. Nucleic Acids Research, 42(16), 10460-10472.

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Culturing Human Adrenocortical Carcinoma Cells

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Two human adrenocortical carcinoma cell lines, H295R and SW13 cells (ATCC, Manassas, Virginia, USA), were used in this study. The cells were cultured at 37°C and 5% CO₂-humidified atmosphere. H295R cells were cultured in Dulbecco’s modified Eagle’s medium/Ham F12 (DMEM/F12) medium, supplemented with 1% ITS Liquid Media Supplement, 100 units/ml of penicillin, 100 μg/ml streptomycin (1% PEST) and 2% Nu-serum. SW13 cells were cultured in DMEM/F12 medium, supplemented with 1% PEST and 10% fetal bovine serum. All reagents were purchased from Thermo Fischer Scientific (Waltham, Massachusetts, USA).

Li S.C., Monazzam A., Razmara M., Chu X., Stålberg P, & Skogseid B. (2021). MiR-486-3p was downregulated at microRNA profiling of adrenals of multiple endocrine neoplasia type 1 mice, and inhibited human adrenocortical carcinoma cell lines. Scientific Reports, 11, 14772.

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Spontaneous Annular Gap Junction Vesicles

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The cell line used in this study was a SW-13 human adrenocortical tumor cell line obtained from ATCC (Manassas, Virginia). These cells form numerous large gap junctions that are spontaneously internalized to form annular gap junction vesicles (Murray et al., 1981 (link)). As previously described, the cells were grown at 37°C under 5% CO₂ in Leibovitz’s L15-medium (Gibco, Grand Island, NY) containing 200 U/ml penicillin, 200 μg/ml streptomycin, 5mg/ml fungizone (Amphotericin B), and 10% fetal calf serum (GIBCO, Grand Island, NY) (Defranco et al., 2008 (link)).

Vanderpuye O.A., Bell C.L, & Murray S.A. (2016). REDISTRIBUTION OF CONNEXIN 43 DURING CELL DIVISION. Cell biology international, 40(4), 387-396.

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Cell Culture Characterization Protocols

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All cell lines used in this study purchased from ATCC cell culture company. African monkey green kidney cells (Vero E6, ATCC CRL-1586), rhesus monkey kidney cells (MA-104, ATCC CRL-2378), human adrenal carcinoma cells (SW-13, ATCC CCL-105) and human cervical adenocarcinoma cells (HeLa, ATCC CCL-2) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma, Germany) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco, USA), 100 mM L-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin (Biological Industries, USA). All cell lines tested were found to be free of mycoplasma using the EZ-PCR Mycoplasma Detection Kit (Biological Industries, USA).

Pavel S.T., Yetiskin H., Aydin G., Holyavkin C., Uygut M.A., Dursun Z.B., Celik İ., Cevik C, & Ozdarendeli A. (2020). Isolation and characterization of severe acute respiratory syndrome coronavirus 2 in Turkey. PLoS ONE, 15(9), e0238614.

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Cell Culture Methodology for ACC Modeling

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Human ACC cell lines NCI-H295R (25 (link)) and SW-13 (26 (link)) and human embryonic kidney cell line HEK-293 (27 (link)) were obtained from ATCC (The ATCC Cell Biology Collection). NCI-H295R, SW-13, and HEK-293 were cultured, respectively, in RPMI medium with 2% fetal bovine serum (FBS) and 1% insulin-transferrin-selenium, L-15 medium with 10% FBS, and DMEM medium with 10% FBS (Gibco, Grand Island, NY, USA) at 37°C in a 95% air-5% CO₂, in fully humidified environment. The culture used was authenticated by STR DNA profiling analysis.

Passaia B.D., Dias M.H., Kremer J.L., Antonini S.R., de Almeida M.Q., Fragoso M.C, & Lotfi C.F. (2018). TCF21/POD-1, a Transcritional Regulator of SF-1/NR5A1, as a Potential Prognosis Marker in Adult and Pediatric Adrenocortical Tumors. Frontiers in Endocrinology, 9, 38.

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Overexpression and Silencing of Key Genes in Adrenal Cell Lines

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Human adrenal normal cell line (Y1) and ACC cell lines (SW-13, NCI-H295R) were obtained from ATCC (Manassas, USA) and cultured in DMEM (Gibco, USA) containing 10% fetal bovine serum (FBS) and 100 U/mL penicillin-100 μg/mL streptomycin (Beyotime, Shanghai, China) in a 5% CO₂ incubator at 37°C.
Full-length cDNAs of human AKR1B10 (overexpression-AKR1B10, Oe-AKR1B10) or HOXA5 (Oe-HOXA5) were cloned into the pcDNA3.1 vector (Thermo Fisher Scientific, Inc.). A pcDNA3.1 empty vector was used as a negative control (Oe-NC). The short hairpin RNA (shRNA) against HOXA5 (shRNA-HOXA5) and negative control shRNA (shRNA-NC) were designed and synthesized by GeneScript (Nanjing, China). NCI-H295R cells in logarithmic growth phase were selected and seeded in the 6-well plates. Cell transfection was performed when the cell confluence was up to 50%-60% according to the instructions of Lipofectamine 2000 (Invitrogen, USA). Following 48 h of transfection, the cells were collected for subsequent experiments.

Chen D., Shen Z., Cheng X., Wang Q., Zhou J., Ren F., Sun Y., Wang H, & Huang R. (2021). Homeobox A5 activates p53 pathway to inhibit proliferation and promote apoptosis of adrenocortical carcinoma cells by inducing Aldo-Keto reductase family 1 member B10 expression. Bioengineered, 12(1), 1964-1975.

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