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Anti bcl11a

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Anti-BCL11A is a laboratory reagent used to detect the BCL11A protein in various biological samples. BCL11A is a transcription factor involved in the regulation of gene expression. This product can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to study the expression and localization of BCL11A in research settings.

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Lab products found in correlation

Mes sds running buffer, by Thermo Fisher Scientific (2 mentions) Anti β actin, by Santa Cruz Biotechnology (2 mentions) Anti ha, by Cell Signaling Technology (2 mentions) Anti nfic, by Cell Signaling Technology (2 mentions) Anti nfia, by Active Motif (2 mentions) Anti nfix clone 3d2, by Merck Group (2 mentions) Anti lrf, by Thermo Fisher Scientific (2 mentions) Anti hemoglobin γ, by Santa Cruz Biotechnology (2 mentions) Mz75 light microscope, by Leica (1 mentions) Bcl11a, by Abcam (1 mentions) Bisbenzimide hoechst 33342, by Merck Group (1 mentions) Af488 or cy3 conjugated secondary, by Merck Group (1 mentions) Orca 285 camera, by Hamamatsu Photonics (1 mentions) Axiophot microscope, by Hamamatsu Photonics (1 mentions)

Close spelling variants of this product

BCL11A (14 citations)

4 protocols using anti bcl11a

1

Immunohistochemical Evaluation of BCL11A

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After dewaxed, rehydrated, and processed for antigen retrieval, the paraffin-embedded sections were quenched with 3% H2O2 and blocked with 3% BSA. The slide was incubated with the primary antibody anti-BCL11A (1:100, Abcam) and with an HRP-conjugated secondary antibody. Staining was visualized by incubation with DAB.
Stained slides were evaluated by 2 experienced pathologists. Based on the staining intensity and the positive rate, a semiquantitative integration method was used to determine the results. The staining intensity score was as follows: colorless = 0, light yellow = 1, yellow-brown = 2, and brown = 3. The percentage of positive cells was scored as follows: < 25% = 1, 26% - 50% = 2, 51% - 75%= 3, >75% = 4. The result of staining was determined using the following formula: score = percentage score × staining intensity score [14 (link), 15 (link)]. Median expression of BCL11A was used as the cutoff and patients were divided into high (n=27) and low (n=26) expression groups.
Jin Q., Chen Y., Du S., Xu D., Yue J., Cai L, & Yuan X. (2022). BCL11A Facilitates Cell Proliferation and Metastasis in Neuroblastoma via Regulating the PI3K/Akt Signaling Pathway. Current Cancer Drug Targets, 22(11), 919-930.
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2

Immunoblotting of Differentiated HUDEP2 Cells

Cited 1 time
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Immunoblotting of proteins were performed by following standard protocol. Briefly, total protein from HUDEP2 cells at day 5 of differentiation or primary cells at day 13 of differentiation were extracted with RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA)58 (link). Equivalent amounts of protein (10-20 µg) were loaded onto 4-12% or 10% Bis-Tris protein gels (Invitrogen) and separated in 1× MES-SDS running buffer (Invitrogen, NP0002). Separated proteins were transferred onto PVDF membrane and incubated with primary antibodies overnight. Primary antibodies used were anti-NFIA (Active Motif, #39397), 1:5,000; anti-NFIX (clone 3D2, Sigma Aldrich, SAB1401263), 1:500; anti-NFIC (Cell Signaling, #11911), 1:1,000; anti-BCL11A (Abcam, ab19487), 1:1,000; anti-LRF (Thermo Fisher Scientific, #14-3309-82), 1:2,000; anti-hemoglobin γ (Santa Cruz, #21756), 1:1,000; anti-β-Actin (Santa Cruz, #47778), 1:1,000; anti-HA (Cell Signaling, #3724), 1:1,000.
Qin K., Huang P., Feng R., Keller C.A., Peslak S.A., Khandros E., Saari M., Lan X., Mayuranathan T., Doerfler P.A., Abdulmalik O., Giardine B., Chou S.T., Shi J., Hardison R.C., Weiss M.J, & Blobel G.A. (2022). Dual function NFI factors control fetal hemoglobin silencing in adult erythroid cells. Nature genetics, 54(6), 874-884.
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3

Immunofluorescence Imaging of Mammary Gland

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Protein samples were prepared as described previously14 (link) and probed using anti-BCL11A (Abcam, Clone 14B5, 1:1000) and Actin (Cell Signalling, 1:10000). For IHC analysis, BCL11A (Abcam (14B5, 1:50); CK14 (Abcam; 1:100) and ERα (SCBT; 1:50) were used. Staining was detected using AF488- or Cy3-conjugated secondary (Sigma) and bisbenzimide-Hoechst 33342 (Sigma). Fluorescence microscopy was carried out using a Zeiss Axiophot microscope equipped with a Hamamatsu Orca 285 camera, with images visualized, captured and manipulated using Simple PCI 6 (C Imaging Systems). The hematoxylin- and eosin-stained samples were visualized on a LEICA light microscope, while the mouse mammary gland whole mounts were visualized using the LEICA MZ75 light microscope.
Khaled W.T., Choon Lee S., Stingl J., Chen X., Raza Ali H., Rueda O.M., Hadi F., Wang J., Yu Y., Chin S.F., Stratton M., Futreal A., Jenkins N.A., Aparicio S., Copeland N.G., Watson C.J., Caldas C, & Liu P. (2015). BCL11A is a triple-negative breast cancer gene with critical functions in stem and progenitor cells. Nature Communications, 6, 5987.
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4

Immunoblotting of Differentiated HUDEP2 Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblotting of proteins were performed by following standard protocol. Briefly, total protein from HUDEP2 cells at day 5 of differentiation or primary cells at day 13 of differentiation were extracted with RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA)58 (link). Equivalent amounts of protein (10-20 µg) were loaded onto 4-12% or 10% Bis-Tris protein gels (Invitrogen) and separated in 1× MES-SDS running buffer (Invitrogen, NP0002). Separated proteins were transferred onto PVDF membrane and incubated with primary antibodies overnight. Primary antibodies used were anti-NFIA (Active Motif, #39397), 1:5,000; anti-NFIX (clone 3D2, Sigma Aldrich, SAB1401263), 1:500; anti-NFIC (Cell Signaling, #11911), 1:1,000; anti-BCL11A (Abcam, ab19487), 1:1,000; anti-LRF (Thermo Fisher Scientific, #14-3309-82), 1:2,000; anti-hemoglobin γ (Santa Cruz, #21756), 1:1,000; anti-β-Actin (Santa Cruz, #47778), 1:1,000; anti-HA (Cell Signaling, #3724), 1:1,000.
Qin K., Huang P., Feng R., Keller C.A., Peslak S.A., Khandros E., Saari M., Lan X., Mayuranathan T., Doerfler P.A., Abdulmalik O., Giardine B., Chou S.T., Shi J., Hardison R.C., Weiss M.J, & Blobel G.A. (2022). Dual function NFI factors control fetal hemoglobin silencing in adult erythroid cells. Nature genetics, 54(6), 874-884.
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