Hplc grade methyl tert butyl ether

Manufactured by Thermo Fisher Scientific

Sourced in United States

HPLC grade methyl tert butyl ether (MTBE) is a solvent used in high-performance liquid chromatography (HPLC) applications. It is a highly pure, distilled product that meets the specifications required for HPLC analysis.

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Lab products found in correlation

Acetonitrile, by Thermo Fisher Scientific (2 mentions) Lc ms grade methanol, by Thermo Fisher Scientific (2 mentions) Formic acid, by Thermo Fisher Scientific (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Ammonium formate, by Thermo Fisher Scientific (1 mentions) Lc ms grade ammonium formate, by Merck Group (1 mentions) 2 propanol, by Thermo Fisher Scientific (1 mentions) Phosphatidylethanolamine, by Avanti Polar Lipids (1 mentions) Sphingomyelin, by Avanti Polar Lipids (1 mentions) Phosphatidylcholine, by Avanti Polar Lipids (1 mentions) Lysophosphatidylcholine, by Avanti Polar Lipids (1 mentions) Phenylhydrazine, by Merck Group (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Cyclophosphamide, by Merck Group (1 mentions) Millipore system, by Merck Group (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Sodium citrate, by Merck Group (1 mentions) Isopropanol, by Thermo Fisher Scientific (1 mentions) Milli q system, by Merck Group (1 mentions)

Close spelling variants of this product

HPLC grade (157 citations) Tert-butyl methyl ether (19 citations) Butyl Ether (3 citations)

5 protocols using hplc grade methyl tert butyl ether

LC/MS-based Lipidomic Analysis Workflow

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Liquid chromatography/mass spectrometry (LC/MS)-grade methanol, acetonitrile, 2-propanol, ammonium formate, and HPLC-grade methyl tert-butyl ether (MTBE) were obtained from Fisher Scientific (PA, USA). LC/MS-grade ammonium formate was from Sigma-Aldrich (MO, USA). A Milli-Q system (MA, USA) was used to prepare ultra-pure water (18.2 MΩ).
Lyso Phosphatidylcholine (LPC 19:0), Phosphatidylethanolamine (PE 12:0/13:0), Ceramide (Cer d18:1/17:0), Sphingomyelin (SM (d18:1/12:0), TG (15:0/15:0/15:0), and Phosphatidylcholine (PC 12:0/13:0) were purchased for use as internal standard (IS) compounds from Avanti Polar Lipids (AL, USA). Antibodies used in this study were rabbit anti-acid Sphingomyelinase polyclonal antibody (Absin, Shanghai, China).

Yang J., Wang M., Yang D., Yan H., Wang Z., Yan D, & Guo N. (2023). Integrated lipids biomarker of the prediabetes and type 2 diabetes mellitus Chinese patients. Frontiers in Endocrinology, 13, 1065665.

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Quantitative Bioanalysis of Cyclophosphamide

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Cyclophosphamide was purchased from Sigma-Aldrich (St. Louis, MO). 4-hydroperoxy-Cyclophosphamide, which is then reduced in water to the 4-hydroxyCyclophosphamide metabolite, was purchased from Asta Medica (Frankfurt, Germany). Deuterated (²[H₄]) Cyclophosphamide (d4-CP) was purchased from Santa Cruz Biotechnology (Dallas, TX), while AZD7451 mesylate was a generous gift from AstraZeneca. All analyte and internal standard structures are presented in Figure 1.
Human whole blood (with EDTA) was provided by the National Institutes of Health Blood Bank. Optima-brand acetonitrile and methanol, and HPLC-grade methyl-tert-butyl ether (MTBE) were purchased from Fisher Scientific (Pittsburgh, PA). Phenylhydrazine, sodium citrate, sodium hydroxide, and formic acid were purchased from Sigma-Aldrich (St. Louis, MO). All water for the assay was deionized and ultra-filtered through a MilliPore system (EMD MilliPore, Billerica, MA).

Hall O.M., Peer C.J., Fitzhugh C.D, & Figg W.D. (2018). A Sensitive and Rapid Ultra High-Performance Liquid Chromatography with Tandem Mass Spectrometric Assay for the Simultaneous Quantitation of Cyclophosphamide and the 4-Hydroxycyclophosphamide Metabolite in Human Plasma. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1086, 56-62.

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Quantitative Analysis of Active Compounds

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Paeoniflorin (C23H28O11, MW: 480.5, purity >98%) and liquiritin (C21H22O9, MW: 418.4, purity >98%), the main active components of Paeonia lactiflora Pall. and Glycyrrhiza uralensis Fisch. ex DC., respectively, were purchased from Nanjing Yuanzhi Biotechnology Co., Ltd. (Nanjing, China). LC-MS grade methanol, acetonitrile, formic acid, and isopropanol were purchased from Fisher Scientific (Waltham, MA, United States). HPLC grade methyl tert butyl ether (MTBE) was obtained from Fisher Scientific (Waltham, MA, United States). The standard lipids were obtained from Avanti Polar Lipids (United States). Deionized water was purified using a Milli-Q system (Millipore, Bedford, MA, United States). All other chemicals and reagents used in this study were of analytical grade.

Chen Y.Y., Feng L.M., Xu D.Q., Yue S.J., Fu R.J., Zhang M.M, & Tang Y.P. (2022). Combination of paeoniflorin and liquiritin alleviates neuropathic pain by lipid metabolism and calcium signaling coordination. Frontiers in Pharmacology, 13, 944386.

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Optimizing Micronutrient Supplementation for Marine Organisms

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Sodium nitrate (plant and cell-culture tested), sodium molybdate dihydrate, copper (II) sulfate pentahydrate, cobalt (II) chloride hexahydrate, butylated hydroxytoluene (BHT) and triethylamine were purchased to Sigma-Aldrich (Arklow, Ireland). Iron (III) chloride hexahydrate, zinc sulphate heptahydrate, manganese (II) chloride tetrahydrate, potassium dihydrogen phosphate and HPLC grade methanol and ethanol 96% were purchased to VWR International Ltd (Dublin, Ireland). Thiamine HCl (vit. B1), biotin (vit. H), cyanocobalamin (vit. B12) and Supelco 37 Component FAME Mix were sourced from Sigma-Aldrich. Tropic Marine Pro-Reef marine salt (Tropic Marin, Wartenberg, Germany) were obtained from a local retailer (McGuire’s Garden Centre, Woodstown, Ireland). Carotenoid standards were obtained from CaroteNature GmbH (Müsingen, Switzerland). The Pierce BCA protein assay kit was obtained from Thermo Fisher Scientific (Dublin, Ireland). The commercial krill and Calanus oil supplements Prowise SUPERBAkrill Oil, Lot 1074600100 (Prowise Healthcare Ltd, London, UK) and Arctic Ruby (Calanus) Oil, Lot 1702071 (Calanus AS, Tromsø, Norway) were purchased online. HPLC grade methyl tert-butyl ether (MTBE) was supplied by Fisher Scientific (Dublin, Ireland).

Prado-Cabrero A., Herena-Garcia R, & Nolan J.M. (2022). Intensive production of the harpacticoid copepod Tigriopus californicus in a zero-effluent ‘green water’ bioreactor. Scientific Reports, 12, 466.

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Quantitative LC-MS Analysis of Analytes

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The list of analytical standards of all target analytes and their deuterated analogues, along with their acronym, CAS number and supplier, can be found in Supplementary Information Tables S6 andS7, respectively. LC-MS water, methanol (MeOH), acetone, hexanes, hydrochloric acid, formic acid, HPLC grade methyl tert-butyl ether (MtBE), pesticide grade isooctane, sulfuric acid, ammonium hydroxide, and hydrochloric acid were all purchased from Fisher Scientific (U.S.A.).
Ultrapure water was prepared using a MilliQ water purification system purchased from Millipore Corporation (Billerica, MA, USA). Oasis HLB, Oasis WAX and glass Oasis HLB glass cartridges were all purchased from Waters (MA, U.S.A.).

Struzina L., Pineda Castro M.A., Kubwabo C., Siddique S., Zhang G., Fan X., Tian L., Bayen S., Aneck-Hahn N., Bornman R., Chevrier J., Misunis M, & Yargeau V. (2022). Occurrence of legacy and replacement plasticizers, bisphenols, and flame retardants in potable water in Montreal and South Africa. The Science of the total environment, 840.

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