Ab9986

Tissue sections were deparaffinized in xylene, then dehydrated in alcohol and incubated in citric acid solution (MXB Biotechnologies, Fuzhou, China) for 10 min at 90 °C to remove surface antigens. After rinsing with PBS, Cell and Tissue Staining Kits (R&D Systems, MN, USA) were used to detect antigens according to the manufacturer's protocols. Tissue sections were stained overnight at 4 °C with an anti-RANKL antibody (1:100; ab45039, Abcam, Cambridge, UK) or an anti-OPG antibody (1:200; ab9986, Abcam, Cambridge, UK), and after rinsing with PBS, they were incubated for 1 h with a secondary antibody (1:2000; ab97240, Abcam, Cambridge, UK; 1:1000 ab6721, Abcam, Cambridge, UK), a fluorescence microscope system (OLYMPUS, Tokyo, Japan) was used to capture images.

The enzyme-linked immunosorbent assay (ELISA) kit for osteocalcin (OCN) (E06917m), Alkaline Phosphatase (ALP) (E0200m), and Tartrate-resistant acid phosphatase (TRAP) (E08492m) were purchased from Elabscience (Wuhan, China). The TRAP staining solution was purchased from Sigma-Aldrich (N0378, USA). Antibody against osteoprotegerin (OPG) (ab9986) and receptor activator of nuclear factor-kB ligand (RANKL) (ab216484) were purchased from Abcam (Cambridge, MA, USA). The low-density lipoprotein cholesterol assay kit (CSB-E13476m), high-density lipoprotein cholesterol assay kit (CSB-E12874m) were purchased from CUSABIO (Wuhan, China). The total cholesterol assay kit (E-BC-K109-S), and triglyceride assay kit (E-BC-K261-M) were purchased from Elabscience (Wuhan, China).

The enzyme-linked immunosorbent assay (ELISA) kit for osteocalcin (OCN) (E06917m), Alkaline Phosphatase (ALP) (E0200m), and Tartrate-resistant acid phosphatase (TRAP) (E08492m) were purchased from Elabscience (Wuhan, China). The TRAP staining solution was purchased from Sigma-Aldrich (N0378, USA). Antibody against osteoprotegerin (OPG) (ab9986) and receptor activator of nuclear factor-kB ligand (RANKL) (ab216484) were purchased from Abcam (Cambridge, MA, USA). The low-density lipoprotein cholesterol assay kit (CSB-E13476m), high-density lipoprotein cholesterol assay kit (CSB-E12874m) were purchased from CUSABIO (Wuhan, China). The total cholesterol assay kit (E-BC-K109-S), and triglyceride assay kit (E-BC-K261-M) were purchased from Elabscience (Wuhan, China).