Treated cells (1 × 106 for AGS and SGC7901) were seeded in triplicate into 96-well plates and allowed to incubate for 24 h, 48 h and 72 h. Cell viability was assessed according to the Cell Counting Kit-8 (CCK-8) protocol (Dojindo, Kumamoto, Japan). For colony formation, treated GC cells were seeded in 6-well plates at 200 cells per well 23 . Two weeks later, colonies were fixed with 4% paraformaldehyde and stained with 0.5% (W/W) crystal violet (diluted in phosphate buffer saline, PBS) for 30 min.
Cell counting kit 8 cck 8 protocol
Manufactured by Dojindo Laboratories
Sourced in Japan
The Cell Counting Kit-8 (CCK-8) is a colorimetric assay used to determine the number of viable cells in cell proliferation and cytotoxicity assays. It utilizes the highly water-soluble tetrazolium salt WST-8, which is reduced by dehydrogenases in viable cells to produce a yellow-colored formazan dye. The amount of formazan dye generated is proportional to the number of living cells.
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Lab products found in correlation
4 protocols using cell counting kit 8 cck 8 protocol
1
Cell Proliferation and Colony Formation Assay
2
Cell Viability Assay with RA16 Aptamer
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NCI-H460 and other cells (2 × 104 cells per well) were seeded in 96-well plates. After 24 hr incubation, cells were treated with RA16 or scrambled aptamer (sequence in Table S2 ) added in fresh medium at different concentrations. Following incubation for 48 hr, cell viability was determined as per the Cell Counting Kit-8 (CCK-8) protocol (Dojindo, Tokyo, Japan). Absorbance was measured at 450-nm wavelength using a microplate reader (Thermo Fisher Scientific, USA).
3
Cisplatin Cytotoxicity Assay in Gastric Cancer
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Stable transfected cells were seeded in 96-well plates at a density of 5 ×103 cells/well and incubated for 24 h. Cell viability was assessed every day or after being treated with cisplatin (25 μM for AGS and MKN28; APExBio, Houston, TX) for 24 h according to the Cell Counting Kit-8 (CCK-8) protocol (Dojindo, Kumamoto, Japan). All experiments were performed at least in triplicate.
4
Cell Viability Assay with CCK-8
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Stable transfected cells (1 × 10 3 for MKN45 and SGC7901) were seeded in triplicate into 96-well plates and allowed to incubate for 24 h. Cell viability was assessed every day according to the Cell Counting Kit-8 (CCK-8) protocol (Dojindo, Kumamoto, Japan).
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