Male c57bl 6 mice

Manufactured by BioLASCO

Sourced in Taiwan, Province of China

Male C57BL/6 mice are a commonly used laboratory mouse strain. They are inbred mice originating from the C57BL/6 parental strain. These mice are commonly used in various research applications.

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Lab products found in correlation

D gal, by Merck Group (1 mentions) Chrysophanol, by Merck Group (1 mentions)

19 protocols using male c57bl 6 mice

High-Fat Diet Interventions and Body Composition in Mice

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The 4‐week‐old C57BL/6 male mice (BioLASCO) were acclimated for 1 week after being delivered to the animal research center. All mice were randomly separated into each cage until the end of the experiment at 10 weeks. The experiment was divided into four groups: control group, HFD group, HFD‐Alg group, and HFD‐TA group. In the control group, mice were daily gavaged with PBS and a normal diet (rodent chow) provided ad libitum. In the HFD group, mice were daily gavaged with PBS and fed with a 60% high‐fat diet (Dyets, DYET# 112252). In the HFD‐Alg group, the mice were daily gavaged with alginate 100 mg/kg and fed with a 60% high‐fat diet. In the HFD‐TA group, the mice were daily gavaged with TA 100 mg/kg and fed with a 60% high‐fat diet. Body weight and food intake were recorded every week.
At the end of the experiment, the whole‐body micro computerized tomography (micro‐CT, SKYSCAN) was used to evaluate body‐fat distribution by resolution of 35 μm.³⁴The mice were sacrificed at 10 weeks. The subcutaneous and epididymis were collected, and the white adipose tissue was harvested and isolated. The overall weight of white adipose tissue was measured and recorded. The GI tract‐related organs and tissues were harvested for histological sectioning and observed under an optical microscope by hematoxylin and eosin staining (H&E stain).

Chen T., Tang R., Lin J., Kuo W., Yang I., Liang Y, & Lin F. (2022). The synthesis and evaluation of thiolated alginate as the barrier to block nutrient absorption on small intestine for body‐weight control. Bioengineering & Translational Medicine, 8(5), e10382.

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Helicobacter pylori Infection in Mice

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Six-week-old, specific-pathogen-free, and Helicobacter-free C57BL/6 male mice were obtained from BioLASCO Taiwan Co., Ltd. and housed at Infectious Disease Core Facility in Biomedical Translation Research Center in Taiwan. H. pylori 26695 and ΔCGT (10¹⁰ bacteria in 200 μl of Brucella broth) were administered by intragastrically for 3 consecutive days for 2 weeks [27 (link), 28 (link)]. Mice were euthanized 10 weeks post-the infection. The stomachs were halved longitudinally, and rinsed in sterile DPBS. Half of the stomach was homogenized using a mechanical homogenizer in Brucella broth containing 10% FBS at room temperature. The appropriate volume of serial dilutions was plated on Brucella agar supplemented with 10% FBS, H. pylori-selective supplement (Dent Oxoid™; SR0147), and 2.5 international unit (IU) ml⁻¹ polymyxin B to determine colonization (CFU) [26 (link)]. The resulting values were normalized by the weight of the fresh tissue used (CFU/mg). The other half of the stomach was formalin-fixed, embedded in optimum cutting temperature compound (OCT), and sectioned to perform immunofluorescence for H. pylori and the autophagy marker LC3.

Muthusamy S., Jan H.M., Hsieh M.Y., Mondal S., Liu W.C., Ko Y.A., Yang W.Y., Mong K.K., Chen G.C, & Lin C.H. (2021). Enhanced enzymatic production of cholesteryl 6ʹ-acylglucoside impairs lysosomal degradation for the intracellular survival of Helicobacter pylori. Journal of Biomedical Science, 28, 72.

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Probiotic Effects on DSS-Induced Colitis

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Six-week old C57BL/6 male mice were used in this study and were purchased from BioLASCO Taiwan Co., Ltd. (Ilan, Taiwan). Animals were maintained in 12 cages (5 animals/cage), in an environment with a relative humidity of 50–60%, temperature of 25 ± 2 °C, and a light/dark cycle of 12 h (illumination between 0700 and 1900 h). All animals received humane care according to guidelines laid out by the Institutional Animal Care and Use Committee of National Taiwan University (Taiwan, ROC). To assess the effect of probiotics on DSS-induced colitis in a mouse model, animals were divided into experimental six groups. These consisted of a control group (control; n = 10), a DSS-induced colitis group with no probiotic treatment (DSS), a DSS-induced colitis group with BCRC 16000 treatment group, a DSS-induced colitis group with BCRC 14023 treatment group, a DSS-induced colitis group with NTU 101 group administered half a dose (NTU 101 0.5 ×), and a NTU 101 group administered a full dose (NTU 101 1.0 ×). The experiment was performed over 25 d. Mice in each group were fed different doses and strains daily. Colitis was induced by DSS added to drinking water on days 15–21. The disease activity index (DAI) was recorded from day 15 onwards. Animals were sacrificed by CO₂ asphyxiation on day 25.

Chen C.L., Hsu P.Y, & Pan T.M. (2018). Therapeutic effects of Lactobacillus paracasei subsp. paracasei NTU 101 powder on dextran sulfate sodium-induced colitis in mice. Journal of Food and Drug Analysis, 27(1), 83-92.

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C57BL/6 Mouse Housing Conditions

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C57BL/6 male mice (BioLasco, Taipei, Taiwan) aged 6 weeks were housed in a light-controlled (14-hour light/10-hour dark cycle) and temperature-controlled (25 ± 2°C) environment for 2 weeks before the experiments and provided with food and water ad libitum. All procedures in this study were reviewed and approved by the Institutional Animal Care and Use Committee (NTU-102-EL-7).

Chiu P.Y., HuangFu W.C., Liu I.H, & Chang Y.P. (2020). Topical application of Heparanase-1 facilitates bone remodeling during the healing of bone defects in a mouse model. Journal of the Chinese Medical Association : JCMA, 83(3).

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Mouse Heart Isolation Protocol

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C57BL/6 male mice (12–14 weeks old) purchased from BioLASCO Taiwan Co. Ltd. were maintained in an artificial 12-h light-dark cycle and given free access to water and food in the Laboratory Animal Center, Tzu Chi University. All experimental and surgical procedures were performed following the recommended procedures approved by the Institutional Animal Care and Use Committee of Tzu Chi University (PPL number: 107104), and conform to the guidelines from Directive 2010/63/EU of the European Parliament on the protection of animals used for scientific purposes or the NIH guidelines. All mice were sacrificed by cervical dislocation. The proceeding for heart isolation was previously described (Chen et al., 2015 (link)).

Chang J.C., Hu W.F., Lee W.S., Lin J.H., Ting P.C., Chang H.R., Shieh K.R., Chen T.I, & Yang K.T. (2019). Intermittent Hypoxia Induces Autophagy to Protect Cardiomyocytes From Endoplasmic Reticulum Stress and Apoptosis. Frontiers in Physiology, 10, 995.

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Mice Handling and Housing Protocol

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Male C57BL/6 mice approximately 25–27 g body weight were purchased from BioLASCO Taiwan Co., Ltd. (Taipei, Taiwan). The animals were maintained in an environment with a 12-h light/dark cycle at 25 ± 1 °C with free access to food and water. The Institutional Animal Care and Use Committee of Taipei Medical University approved all animal use protocols (LAC-2017-0182, 25 July 2017), and animals were operated in accordance with guidelines of ARVO statement for ophthalmic and vision research.

Lin F.L., Yen J.L., Kuo Y.C., Kang J.J., Cheng Y.W., Huang W.J, & Hsiao G. (2019). HDAC8 Inhibitor WK2-16 Therapeutically Targets Lipopolysaccharide-Induced Mouse Model of Neuroinflammation and Microglial Activation. International Journal of Molecular Sciences, 20(2), 410.

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Animal Welfare-Compliant Mice Study

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Male C57BL/6 mice (10–12 weeks of age) were purchased from BioLASCO Taiwan Co., Ltd. (Taipei, Taiwan). All protocols involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of Chang Gung Memorial Hospital. The animal experiments were conducted in strict accordance with the ethical principles outlined in the Animal Welfare Act and the Guide for the Care and Use of Laboratory Animals issued by the National Institutes of Health. The animals were housed in environments with controlled conditions and were maintained under a 12 h light/12 h dark cycle at the Laboratory Animal Center of Chang Gung Memorial Hospital. The animals underwent an overnight fasting period prior to the experiments.

Liu F.C., Yu H.P., Lee H.C., Chen C.Y, & Liao C.C. (2024). The Modulation of Phospho-Extracellular Signal-Regulated Kinase and Phospho-Protein Kinase B Signaling Pathways plus Activity of Macrophage-Stimulating Protein Contribute to the Protective Effect of Stachydrine on Acetaminophen-Induced Liver Injury. International Journal of Molecular Sciences, 25(3), 1484.

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C57BL/6 Mice IACUC Study

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Male C57BL/6 mice (BioLASCO Taiwan Co., Ltd.) were recruited for the experiments. These mice arrived at the housing environment at six-week ages, ranging from 22 to 26 g. The first week after arrival was the habituation period. The housing environment was set in a 12-h:12-h light:dark cycle. Food and water supply were ad libitum. The following experiment protocol has been approved by the Institutional Animal Care and Use Committee (IACUC), and the approval number is NTU-110-EL-00156. The sample sizes (n) for the vehicle-control group (Ctrl_BLA_Veh), the vehicle-MPS group (MPS_BLA_Veh), and the TCS-MPS group (MPS_BLA_TCS) are 11, 7, and 10, respectively.

Lee T.Y., Yi P.L, & Chang F.C. (2023). Hypocretin role in posttraumatic stress disorder-like behaviors induced by a novel stress protocol in mice. Frontiers in Psychiatry, 14, 1196994.

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D-gal-induced Aging Model in Mice

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Male C57BL/6 mice (7-8 weeks old, weighing 20 ± 1.6 g) were purchased from BioLASCO Corp (Yilan, Taiwan). The animals were housed under the following conditions: 23 ± 2°C with a relative humidity of 60 ± 10%. They were fed a standard diet and water ad libitum throughout the experimental period. All experiments were conducted in accordance with the guidelines of the Institutional Animal Care and Use Committee (IACUC) of the Agricultural Technology Research Institute. This study was approved by the IACUC ethics committee, under protocol 109025.
Six to seven mice were randomly assigned to each of four groups: D-gal group (D-gal treated only), D-gal/CEr, D-gal/CEw, and D-gal/CEp groups. The control group was subcutaneously injected with the same volume of physiological saline. All groups were injected intraperitoneally with D-gal (200 mg/kg, Sigma, St Louis, MO, USA) once daily, for 120 days. Meanwhile, D-gal plus different embryonic extracts of CEr, CEw, or Cep were oral gavage administered for 120 days. In addition, 10-11 week-old mice (6 mice in this group) were used as a young control group. At the end of the experiments, all mice were killed via asphyxiation using 95% CO₂, and the serum, organs, and tissues of mice were immediately collected for different assays and analyses.

Hong H.T., Hsu T.H., Hung S.W., Chiu C.C., Wu C.Y., Chen C.C., Lee G.C., Lin J.S, & Wu C.P. (2021). Different Parts of the Chicken Embryo Egg Improve D-Galactose-Induced Aging in a Mice Model. BioMed Research International, 2021, 6654683.

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MPTP-Induced Parkinson's Mouse Model

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Male C57BL6 mice (aged 4–8 weeks; BioLasco Taiwan Co., Ltd., Taipei, Taiwan) were used. They were housed in our animal facility under a 12 h light/dark cycle with food and water available ad libitum for at least 4 days before the experiments. The animal use protocol (China Medical University protocol number: 2016-198) was reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) of China Medical University according to the principles of the 3Rs (replacement, reduction, and refinement). MPTP and celastrol dosages for mice studies were according to the previous studies [3 (link),20 (link)]. Mice received intraperitoneal (i.p.) injections of MPTP (10 mg/kg/day for 3 days) 24 h after the last celastrol injection (3 mg/kg/day for 3 days; Figure 3A).

Lin M.W., Lin C.C., Chen Y.H., Yang H.B, & Hung S.Y. (2019). Celastrol Inhibits Dopaminergic Neuronal Death of Parkinson’s Disease through Activating Mitophagy. Antioxidants, 9(1), 37.

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