Sodium cholate hydrate (NaC), sodium taurocholate hydrate (NaTC), sodium deoxycholate (NaDC), and sodium taurodeoxycholare (NaTDC) from Sigma-Aldrich were used as received. Solutions at concentration 40, 50, 200 mM, depending on the experiment, were prepared using boiled doubly distilled water or in 100 mM acetate buffer at pH = 3, in which only NaTC and NATDC are soluble.
Alginic acid sodium salt from brown algae was supplied by Sigma-Aldrich. Low methoxyl pectin from citrus peels (CU701, DE = 38%) was provided by Herbstreit & Fox Neuenbuerg (Germany). Chitosan, (batch 34480004, 91.1% deacetylated) was supplied by ACEF, Fiorenzuola d’Arda (PC, Italy). All the SDF were used as received. 1% w/v SDF solutions were prepared by weight and stirred overnight at a constant rate.
Alginic acid sodium salt from brown algae was supplied by Sigma-Aldrich. Low methoxyl pectin from citrus peels (CU701, DE = 38%) was provided by Herbstreit & Fox Neuenbuerg (Germany). Chitosan, (batch 34480004, 91.1% deacetylated) was supplied by ACEF, Fiorenzuola d’Arda (PC, Italy). All the SDF were used as received. 1% w/v SDF solutions were prepared by weight and stirred overnight at a constant rate.