To further explore the effect of different cultivation methods on the accumulation of metabolites, the metabolomics analysis was conducted on the water dropwort cultivated in the optimal nutrient solution formula and soil culture. After harvesting, water dropwort under soil culture (CK) and nutrient solution culture (NK) were selected for broadly targeted metabolomics detection. The freeze-dried water dropwort leaf was crushed using a mixer mill (MM 400, Retsch, Germany) with a zirconia bead, for 1.5 min at 30 Hz. Next, 100 mg of powder was weighed and extracted overnight at 4 °C with 0.6 mL of 70% methanol aqueous solution. After centrifugation at 10,000× g for 10 min, the extracts were absorbed (CNWBOND Carbon−GCB SPE Cartridge, 250 mg, 3 mL; ANPEL, Shanghai, China) and filtrated (SCAA−104, 0.22 μm pore size; ANPEL, Shanghai, China), before UPLC−MS/MS analysis [63 (link)].
Cnwbond carbon gcb spe cartridge
Manufactured by ANPEL
Sourced in China
The CNWBOND Carbon-GCB SPE Cartridge is a solid-phase extraction (SPE) device designed for the purification and concentration of analytes. It features a sorbent bed composed of both carbon and graphitized carbon black (GCB) materials, which provide selective retention of target compounds. The core function of this product is to facilitate the extraction, cleanup, and pre-concentration of samples prior to instrumental analysis.
Automatically generated - may contain errors
Lab products found in correlation
Scaa 104, by ANPEL (70 mentions)
Acquity uplc hss t3 c18, by Waters Corporation (6 mentions)
Shim pack uflc cbm30a system, by Shimadzu (5 mentions)
4500 q trap, by Thermo Fisher Scientific (3 mentions)
6500 q trap, by Thermo Fisher Scientific (3 mentions)
Scientz 100f, by Ningbo Scientz Biotechnology (3 mentions)
Hplc grade, by Merck Group (2 mentions)
Cbm30a system, by Thermo Fisher Scientific (2 mentions)
Scaa 104 filter, by ANPEL (2 mentions)
Applied biosystems 6500 q trap, by Thermo Fisher Scientific (1 mentions)
6500 triple quadrupole, by Thermo Fisher Scientific (1 mentions)
Exionlc ad, by Thermo Fisher Scientific (1 mentions)
Guaranteed reagent, by Merck Group (1 mentions)
Qtrap 6500, by Thermo Fisher Scientific (1 mentions)
Acquity uplc hss t3, by Waters Corporation (1 mentions)
83 protocols using cnwbond carbon gcb spe cartridge
1
Metabolomic Analysis of Water Dropwort Cultivation
2
Analytical Workflow for Flower Metabolites
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Fig female ower samples were further ground and thoroughly mixed with a mortar and pestle in liquid nitrogen. The freeze-dried tissue was crushed using a mixer mill (MM 400, Retsch) with Zirconia beads for 1.5 min at 30 Hz. The powdered sample (100 mg) was weighed and extracted overnight at 4 °C with 1.0 mL 70% aqueous methanol. Following centrifugation at 10,000 × g for 10 min, the extracts were absorbed (CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3 mL; ANPEL, Shanghai, China, www.anpel.com.cn/cnw) and ltrated (SCAA-104, 0.22 µm pore size; ANPEL) before LC-MS analysis.
The g female ower sample extracts were analyzed using an LC-ESI-MS/MS system (HPLC, Shim-pack UFLC SHIMADZU CBM30A system, www.shimadzu.com.cn; MS, Applied Biosystems 6500 Q TRAP, www.appliedbiosystems.com.cn). The analytical conditions were as follows: HPLC column, Waters ACQUITY UPLC HSS T3 C18 (1.8 µm, 2.1 mm ⋅ 100 mm); the solvent system was water (0.04% acetic acid) and acetonitrile (0.04% acetic acid); gradient program, 95:5 V/V at 0 min, 5:95 V/V at 11.0 min, 5:95 V/V at 12.0 min, 95:5 V/V at 12.1 min, 95:5 V/V at 15.0 min; ow rate, 0.40 ml/min; temperature, 40 °C; injection volume, 2 µL. The e uent was alternatively connected to an ESI-triple quadrupole-linear ion trap (Q TRAP)-MS [26].
The g female ower sample extracts were analyzed using an LC-ESI-MS/MS system (HPLC, Shim-pack UFLC SHIMADZU CBM30A system, www.shimadzu.com.cn; MS, Applied Biosystems 6500 Q TRAP, www.appliedbiosystems.com.cn). The analytical conditions were as follows: HPLC column, Waters ACQUITY UPLC HSS T3 C18 (1.8 µm, 2.1 mm ⋅ 100 mm); the solvent system was water (0.04% acetic acid) and acetonitrile (0.04% acetic acid); gradient program, 95:5 V/V at 0 min, 5:95 V/V at 11.0 min, 5:95 V/V at 12.0 min, 95:5 V/V at 12.1 min, 95:5 V/V at 15.0 min; ow rate, 0.40 ml/min; temperature, 40 °C; injection volume, 2 µL. The e uent was alternatively connected to an ESI-triple quadrupole-linear ion trap (Q TRAP)-MS [26].
3
Targeted Metabolic Profiling by LC-MS/MS
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Metabolite profiling was performed using a widely targeted metabolome method by Wuhan Metware Biotechnology Co., Ltd. (Wuhan, China). The freeze-dried samples were extracted as previously described and then analyzed using an LC–electrospray ionization (ESI)-MS/MS system. The extracts were absorbed with the CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3 mL (Shanghai ANPEL Scientific Instrument Co., Ltd., Shanghai, China). The metabolites were quantified using the multiple reaction monitoring (MRM) method.
4
Extraction and Analysis of FaGAPCp1 Protein
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The freeze-dried FaGAPCp1 overexpressing fruits were crushed using a mixer mill (MM 400, Retsch) with a zirconia bead for 1.5 min at 30 Hz. 100 mg powder was weighted and extracted overnight at 4 °C with 1.0 mL 70% aqueous methanol. After centrifugation at 10,000× g for 10 min, the extracts were absorbed (CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3 mL; ANPEL, Shanghai, China) and filtered (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai, China) and then subjected to LC-MS analysis.
5
Extraction and Purification of S. baicalensis Compounds
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The vacuum freeze-dried S. baicalensis specimens were ground to powder using a mixer mill (MM 400, Verder Retsch, Shanghai, China) at 30 Hz for 90 s. During extraction, 100 mg of powder was weighed and dissolved in 1 mL aqueous methanol (70%). Subsequently, the mixture was incubated overnight at 4°C in a refrigerator. During incubation, the samples were swirled three times at 10-min intervals (10 s, 40 Hz) to improve the extraction rate. After centrifugation (10,000 g, 10 min), the extract was absorbed using a CNWBOND Carbon-GCB SPE Cartridge (250 mg, 3 mL; ANPEL, Shanghai, China)1 and filtrated (SCAA-104, 0.22-μm pore size; ANPEL, Shanghai, China)(see text footnote 1) prior to ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis.
6
Quantitative Extraction of Floral Compounds
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The freeze-dried flower buds were crushed using a mixer mill (MM 400, Retsch Haan, Germany) with a zirconia bead for 1.5 min at 30 Hz. Then, 100mg powder was weighted and extracted overnight at 4 °C with 1.0 mL 70% aqueous methanol. Following centrifugation at 10,000× g for 10 min, the extracts were absorbed (CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3 mL; ANPEL, Shanghai, China) and filtrated (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai, China) before LC-MS analysis.
7
Extraction and Analysis of Phytochemicals
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The freeze-dried leaf was crushed using a mixer mill (mm 400, retsch) with a zirconia bead for 1.5 min at 30 hz. 100 mg powder was weighted and extracted overnight at 4 °C with 1.0 mL 70% aqueous methanol. Following centrifugation at 10, 000g for 10 min, the extracts were absorbed (CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3ml; ANPEL, Shanghai, China) and filtrated (SCAA-104, 0.22μm pore size; ANPEL, Shanghai, China) before LC-MS analysis.
8
Leaf Metabolites Extraction and Analysis
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The freeze-dried leaf was crushed using a mixer mill (MM 400, Retsch) with a zirconia bead for 1.5 min at 30 Hz. 100 mg powder was weighted and extracted overnight at 4℃ with 0.6 ml 70% aqueous methanol. Following centrifugation at 10, 000 g for 10 min, the extracts were absorbed (CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3ml; ANPEL, Shanghai, China, www.anpel.com.cn/cnw ) and filtrated (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai, China, http://www.anpel.com.cn/ ) before UPLC-MS/MS analysis.
9
Leaf Powder Extraction for LC-MS Analysis
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Freeze-dried leaves were crushed using a mixer mill (MM 400, Retsch) with a zirconia bead for 1.5 min at 30 Hz. Then, 100 mg of powder was weighed and extracted overnight at 4°C with 1.0 mL of 70% aqueous methanol. Following centrifugation at 10, 000 g for 10 min, the extracts were absorbed (CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3 mL; ANPEL, Shanghai, China, www.anpel.com.cn/cnw ) and filtered (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai, China, http://www.anpel.com.cn/ ) before LC-MS analysis.
10
Metabolome Analysis of 'Big Five-pointed Star'
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A total of nine samples (three from leaves, three from flowers, and three from roots) of the ‘Big Five-pointed Star’ cultivar were collected at different developmental stages and subjected to metabolome analysis. The freeze-dried samples were crushed using an MM 400 mixer mill (Retsch, Haan, Germany) and zirconia beads for 1.5 min at 30 Hz. Then, 100 mg of each sample was subjected to 70% methanol extraction overnight at 4 °C. After centrifugation at 10,000 × g for 10 min, the extracts were absorbed (CNWBOND Carbon-GCB SPE Cartridge, 250 mg, 3 mL; ANPEL Laboratory Technologies, Shanghai, China) and filtered (SCAA-104, 0.22 μm pore size; ANPEL) before being subjected to UPLC-MS/MS analysis.
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