Log In Sign up
The largest database of trusted experimental protocols

Rs 2000 biological irradiator

Manufactured by Rad Source
Visit Supplier
Sourced in United States, Canada

The RS-2000 biological irradiator is a self-shielded gamma irradiator designed for research and medical applications. It utilizes Cobalt-60 as the radiation source and provides a controlled environment for the irradiation of biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Dissecting microscope, by Leica (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Streptavidin conjugated microbeads, by Miltenyi Biotec (2 mentions) X tremegene 9 dna transfection reagent, by Roche (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) B6.129 cg foxp3tm4 yfp icre ayr j, by Jackson ImmunoResearch (1 mentions) B6 sjl ptprca pepcb boyj, by Jackson ImmunoResearch (1 mentions) B6 cg foxp3sf j, by Jackson ImmunoResearch (1 mentions) B6.129s7 rag1tm1mom j, by Jackson ImmunoResearch (1 mentions) U87 human glioblastoma cell line, by American Type Culture Collection (1 mentions) Abt 888, by Enzo Life Sciences (1 mentions) Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions) Microvascular endothelial cell growth medium, by Cell Applications (1 mentions) Hlmvec, by Cell Applications (1 mentions) Facscalibur, by BD (1 mentions) Ha cell line, by ScienCell (1 mentions) Astrocyte medium, by Thermo Fisher Scientific (1 mentions) Snb19, by American Type Culture Collection (1 mentions) Lumina xr imaging system, by PerkinElmer (1 mentions)

Close spelling variants of this product

RS-2000 irradiator (30 citations) RS 2000 X-ray Biological Irradiator (26 citations) RS 2000 Biological Research Irradiator (13 citations) RS 2000 Biological Research X-ray Irradiator (6 citations) RS-2000 Pro Biological Irradiator (4 citations) RS 2000 Biological System irradiator (3 citations) Radsource RS-2000 Biological Irradiator (2 citations) RadSource RS 2000 Biological Research Irradiator (2 citations) RS-2000 XE Biological Irradiator (2 citations) RS 2000 biological cabinet X-Irradiator (2 citations)

50 protocols using rs 2000 biological irradiator

1

Dose-Dependent Radiation Effects on GSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were irradiated using the RS-2000 Biological Irradiator (RadSource, Buford, GA, USA) at various dosages per equipment specifications. For dose-dependent radiation effects on GSC viability without addition of 2-DG, 2 Gray (Gy) to 20 Gy was administered, and 48 h post treatment, trypan blue was used to differentiate alive versus dead cells. For all transmission electron microscopy experiments, combination-therapy viability experiments, and western blot experiments, a single-time dose of 8 Gy was utilized.
Shah S.S., Rodriguez G.A., Musick A., Walters W.M., de Cordoba N., Barbarite E., Marlow M.M., Marples B., Prince J.S., Komotar R.J., Vanni S, & Graham R.M. (2019). Targeting Glioblastoma Stem Cells with 2-Deoxy-D-Glucose (2-DG) Potentiates Radiation-Induced Unfolded Protein Response (UPR). Cancers, 11(2), 159.
+ Open protocol
+ Expand
2

Xenograft Model of Radiosensitive Tumors

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Female 4–5-week old BALB/c nude mice (18–20 g) were assessed. Xenografts of TE-13 cells in mice were obtained by subcutaneously injecting 0.1 mL of normal TE-13 (n=12) or CBX4-knockdown TE-13 (n=12) cells (106 cells) into the right proximal hindlimb. The mice were randomly allocated to 4 groups (n=6), including the Control, CBX4-Knockdown, Control + IR, and CBX4-Knockdown + IR groups. Tumors were assessed at 3-day intervals with a Vernier caliper, and tumor volume (V) was determined as V=(a × b2) ×0.5 (a is the long diameter, and b is the short diameter). For irradiation groups, a RS-2000 biological irradiator (Rad Source Technologies, Suwanee, GA, USA) was employed for irradiation at 6 Gy by X-rays (2 Gy/min) when the tumor volume reached about 80 mm3. At 15 d following cancer cell injection, the mice were euthanized, followed by tumor extraction and weighing (18 (link)). Experiments were performed under a project license (No. 050432-4-1212B) granted by The Ethics Committee of Fudan University Shanghai Cancer Center, in compliance with Animal [Scientific Procedures] Act 1986, national or institutional guidelines for the care and use of animals.
Zhu H., Chen H., Chen G., Liu M., Chu L., Gu Y., Chen Y., Zhang C., Qin Q., Chen Y., Chen W., Fan J., Nie Y., Chen J., Wu S., Sun X., Zhao W, & Zhao K. (2022). CBX4 contributes to radioresistance by regulating autophagic activity in esophageal squamous cell carcinoma. Annals of Translational Medicine, 10(18), 959.
+ Open protocol
+ Expand
3

Genetic Mouse Models for Treg Cell Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
B6.129(Cg)-Foxp3tm4(YFP/icre)Ayr/J (Foxp3Cre, strain 016959), B6.SJL-PtprcaPepcb/BoyJ (CD45.1 congenic mice, strain 002014), B6.129S7-Rag1tm1Mom/J (Rag1−/− mice, strain 002216), and B6.Cg-Foxp3sf/J (B6-scurfy mice, strain 004088) were obtained from Jackson Laboratory. Tet2fl/flTet3fl/flFoxp3Cre mice were generated in our laboratory by crossing Tet2fl/flTet3fl/f with Foxp3Cre mice. For the fate-mapping experiments, Tet2+/flTet3fl/flFoxp3Cre or Tet2fl/flTet3+/flFoxp3Cre female mice were crossed with Tet2fl/flTet3fl/flRosa26-YFP+ male mice to generate Tet2fl/flTet3fl/flFoxp3CreRosa26-YFP+ male mice for further analysis. All mice were on the B6 background and maintained in a specific pathogen-free animal facility in the La Jolla Institute for Immunology. Age of the mice used for each experiment was stated in the figure legends. All cell or mouse irradiation procedures were performed using RS2000 Biological Irradiator (Rad Source Technologies, Inc.) All animal procedures were reviewed and approved by the Institutional Animal Care and Use Committee of the La Jolla Institute for Immunology and were conducted in accordance with institutional guidelines.
Yue X., Lio C.W., Samaniego-Castruita D., Li X, & Rao A. (2019). Loss of TET2 and TET3 in regulatory T cells unleashes effector function. Nature Communications, 10, 2011.
+ Open protocol
+ Expand
4

Cell Line Establishment and Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
Stable MCF7/E6 and their associated control cell line were established from cells purchased from ATCC as described previously (15 (link)). The U87 human glioblastoma cell line was purchased from ATCC. SF767 shCtrl and SF767 shp53 human glioma cell lines were a gift from Dr. Jay Fitzgerald Dorsey (Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA) and have been previously described (23 (link)). Cell lines were cultured at low passage number and authenticated by morphology and growth characteristics. Cells were tested for Mycoplasma by PCR (ATCC) every three months. All cell lines were maintained in DMEM supplemented with 10% fetal bovine serum (Sigma) and 1% penicillin/streptomycin. Transient p53 knockdown was accomplished using p53-targeting siRNAs (L-003329–00, Dharmacon) with siRNA A4 (Dharmacon) serving as a non-targeting control and Lipofectamine2000 transfection reagent (Invitrogen) as recommended by the manufacturer. Cells were irradiated as indicated using a RS-2000 biological irradiator (Rad Source). Cells were treated with ABT-888 (Enzo) as described in the text.
Sizemore S.T., Mohammad R., Sizemore G.M., Nowsheen S., Yu H., Ostrowski M.C., Chakravarti A, & Xia F. (2018). Synthetic Lethality of PARP Inhibition and Ionizing Radiation is p53-dependent. Molecular cancer research : MCR, 16(7), 1092-1102.
+ Open protocol
+ Expand
5

BM Chimera Generation for Radiation Study

Check if the same lab product or an alternative is used in the 5 most similar protocols
For BM chimeras, recipients were irradiated with two 6 Gray doses of x-ray total body irradiation separated by 3 h. The source of ionizing radiation was an x-ray generator (RS-2000 Biological Irradiator; Rad Source Technologies, Inc.) operating at 160 kV and 25 mA, yielding an absorbed dose rate of 2.2 Gy/min. 16 h after irradiation, BM was removed by flushing out femoral bones from donor mice with 5 ml of ice-cold RPMI media under sterile conditions. Cells were disaggregated with a 22-gauge needle and filtered through a sterile 40-µm cell strainer, and cells were resuspended in RPMI medium. Mice received 5–10 × 106 BM cells by retroorbital injection. Chimeras were analyzed 7 wk after transplantation.
Zamora-Pineda J., Kumar A., Suh J.H., Zhang M, & Saba J.D. (2016). Dendritic cell sphingosine-1-phosphate lyase regulates thymic egress. The Journal of Experimental Medicine, 213(12), 2773-2791.
+ Open protocol
+ Expand
6

Isolation and Irradiation of Placental Mesenchymal Stem Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Fetal deciduous placental tissues were obtained from a healthy mother after informed consent. The pMSCs were isolated and expanded according to a previously reported protocol,9 (link) and these were cultured in low-glucose Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher Scientific, Waltham, MA, USA) containing 10% fetal bovine serum (Thermo Fisher Scientific), penicillin (100 U/mL), and streptomycin (100 μg/mL). Purified pMSCs at passages 8–10 and grown to 85%–90% confluency (Figure S1A and B) were subjected to 80, 160, 240, and 320 Gy irradiation using RS-2000 biological irradiator (RadSource), then stored frozen at −80°C for the entire series of experiments. Lewis lung carcinoma (LL2) cell line was obtained from American Type Culture Collection (Manassas, VA, USA) and cultured in DMEM/F12 integrative medium (Thermo Fisher Scientific) for spheroid formation with stem cell-like transition. The spheroid-bearing LL2 cells at logarithmic phase were harvested and irradiated for the experiments (Figure S2A–E).
Zhang Y.N., Duan X.G., Zhang W.H., Wu A.L., Yang H.H., Wu D.M., Wei Y.Q, & Chen X.C. (2016). Antitumor activity of pluripotent cell-engineered vaccines and their potential to treat lung cancer in relation to different levels of irradiation. OncoTargets and therapy, 9, 1425-1436.
+ Open protocol
+ Expand
7

Transfection and Irradiation of Tumor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
LL/2 tumor cells were respectively transfected with MCS, MCS-mGM-CSF, MCS-mIL-18 and MCS-mGM-CSF + IL-18 plasmids by Cationic liposome (DOTAP-Chol: DNA = 6:1). For 48 hours, the tumor cells were extensively digested and washed three times, then suspended in 1 ml serum free DMEM medium. The cell resuspension in each group was irradiated with a sublethal dose X-ray (100 Gy) [27 (link)] by irradiator (RS-2000 biological irradiator, Rad Source Technologies, Inc. Suwanee, GA). Irradiated tumor cells were used for further study, including morphologic observation, proliferation assay, detection of cytokine levels and animal experiments.
Tian H., Shi G., Yang G., Zhang J., Li Y., Du T., Wang J., Xu F., Cheng L., Zhang X., Dai L., Chen X., Zhang S., Yang Y., Yu D., Wei Y, & Deng H. (2014). Cellular immunotherapy using irradiated lung cancer cell vaccine co-expressing GM-CSF and IL-18 can induce significant antitumor effects. BMC Cancer, 14, 48.
+ Open protocol
+ Expand
8

Oral Mucositis in Tongue Tumor Mice

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice with tongue tumors (7 days after injection of cancer cells) were cranially irradiated to induce oral mucositis using a RS2000 biological irradiator (Rad Source Technologies) as previously reported (10 (link)). Based on our previous observation that a single 18Gy dose has kinetics and severity of oral mucositis similar to 8Gy x3 fractionated irradiation (10 (link)), we chose 18Gy irradiation to reduce the death rate due to repeated anesthesia for fractionated irradiation to oral tumor-bearing mice that had deteriorating health. Each mouse was anesthetized with 80 mg/kg ketamine, 12 mg/kg xylazine and placed under a lead shield exposing only their head. The day of irradiation was designated day 1. All animals were provided soft food in addition to standard diet. On day 6, when mice began to lose weight due to oral ulcer-associated reduced food intake, mice were divided into 2 groups (of equal weight and tumor size) and treated daily with Tat-Smad7 or vehicle. 125 mg/kg BrdU was administered i.p. two hours before euthanasia. Mice were sacrificed and tongue samples collected on day 10 for pathological evaluation and immunostaining.
Luo J.J., Bian L., Blevins M.A., Wang D., Liang C., Du D., Wu F., Holwerda B., Zhao R., Raben D., Zhou H., Young C.D, & Wang X.J. (2018). Smad7 Promotes Healing of Radiotherapy-Induced Oral Mucositis without Compromising Oral Cancer Therapy in a Xenograft Mouse Model. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(2), 808-818.
+ Open protocol
+ Expand
9

Clonogenic Assay for Radiation Response

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Radiation clonogenic assay was performed as previously described (16 (link)). Briefly, cells were trypsinized to generate a single cell suspension and seeded in 60 mm tissue culture plates in triplicate. Cells were incubated with DMSO or Torin2 for 3 hours and then irradiated with various doses (0–8 Gy). Radiation was performed with 160kV, 25mA at a dose rate of approximately 113cGy/min using a RS-2000 biological irradiator with a 0.3 mm copper filter (RadSource, Buford, GA). Twenty-four hours after radiation, medium was replaced with fresh medium without DMSO or Torin2. Seven to ten days after seeding, colonies were fixed with methanol/acetic acid, and stained with 0.5% crystal violet. The number of colonies containing at least 50 cells was counted using a dissecting microscope (Leica Microsystems, Inc. Buffalo Grove, IL). Experiments were repeated at least 3 independent times and a representative experiment was chosen.
Shen C., Shyu D.L., Xu M., Yang L., Webb A., Duan W, & Williams T.M. (2022). Deregulation of AKT-mTOR Signaling Contributes to Chemoradiation Resistance in Lung Squamous Cell Carcinoma. Molecular cancer research : MCR, 20(3), 425-433.
+ Open protocol
+ Expand
10

Irradiation of Human Lung Microvascular Endothelial Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Human lung microvascular endothelial cells (HLMVEC) were purchased from Cell Applications (San Diego, CA, USA), cultured on plates treated with endothelial cell attachment factor in Microvascular Endothelial Cell Growth Medium (Cell Applications) in a humidified environment of 5% CO2/95% air at 37 °C, according to the manufacturer’s instructions. Cells were used within seven passages for all experiments. Cells were irradiated at 70–90% confluence using an RS2000 Biological Irradiator (Rad Source Technologies, Alpharetta, GA, USA) at a dose rate of 1.15 Gy/min (160 kV, 25 mA) for a total dose of 10 Gy as previously described24 (link),30 (link).
Bouten R.M., Dalgard C.L., Soltis A.R., Slaven J.E, & Day R.M. (2021). Transcriptomic profiling and pathway analysis of cultured human lung microvascular endothelial cells following ionizing radiation exposure. Scientific Reports, 11, 24214.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!