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300 mesh

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The Quantifoil 300 mesh is a laboratory equipment product designed for microscopy applications. It provides a grid-like structure with uniform holes, allowing for the support and analysis of various samples. The core function of this product is to facilitate the preparation and examination of specimens under a microscope.

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Lab products found in correlation

Titan krios g3, by Thermo Fisher Scientific (4 mentions) Vitrobot mark 4, by Thermo Fisher Scientific (4 mentions) Mark 4, by Thermo Fisher Scientific (3 mentions) K3 direct detection camera, by Thermo Fisher Scientific (1 mentions)

8 protocols using 300 mesh

1

Cryo-EM imaging of HTT and HTT-HAP40

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HTT was diluted to 0.4 mg/mL in 20 mM HEPES pH 7.5, 300 mM NaCl, 1 mM TCEP and adsorbed to glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3) for 10 s. Grids were then blotted with filter paper for 2 s at 100% humidity at 4 °C and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific).
HTT-HAP40 was diluted to 0.2 mg/mL in 25 mM HEPES pH 7.4, 300 mM NaCl, 0.025% w/v CHAPS and 1 mM DTT and adsorbed onto gently glow-discharged suspended monolayer graphene grids (Graphenea) for 60 s. Grids were then blotted with filter paper for 1 s at 100% humidity, 4 °C and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific).
Data were collected in counting mode on a Titan Krios G3 (FEI) operating at 300 kV with a BioQuantum imaging filter (Gatan) and K2 direct detection camera (Gatan) at ×165,000 magnification, pixel size of 0.822 Å. Movies were collected over 32 fractions at a dose rate of 6.0 e−/Å2/s, exposure time of 8 s, resulting in a total dose of 48.0 e−/Å2.
Harding R.J., Deme J.C., Hevler J.F., Tamara S., Lemak A., Cantle J.P., Szewczyk M.M., Begeja N., Goss S., Zuo X., Loppnau P., Seitova A., Hutchinson A., Fan L., Truant R., Schapira M., Carroll J.B., Heck A.J., Lea S.M, & Arrowsmith C.H. (2021). Huntingtin structure is orchestrated by HAP40 and shows a polyglutamine expansion-specific interaction with exon 1. Communications Biology, 4, 1374.
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2

Cryo-EM Preparation of Vag8–C1-INH Complex

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Four microliters of purified Vag8–C1-INH complex (0.5 mg/ml) was adsorbed to glow-discharged holey carbon-coated grids (Quantifoil 300 mesh; Au R1.2/1.3) for 10 s. Grids were then blotted for 3 s at 100% humidity at 8°C and frozen in liquid ethane using a Vitrobot Mark IV (FEI).
Dhillon A., Deme J.C., Furlong E., Roem D., Jongerius I., Johnson S, & Lea S.M. (2021). Molecular Basis for Bordetella pertussis Interference with Complement, Coagulation, Fibrinolytic, and Contact Activation Systems: the Cryo-EM Structure of the Vag8-C1 Inhibitor Complex. mBio, 12(2), e02823-20.
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3

Cryo-EM Structure Determination of GldLM′ and PorLM

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Four microlitre aliquots of purified GldLM′ (A280nm = 0.4-0.5) or PorLM (A280nm = 0.2-0.5) complexes were applied onto glow-discharged holey carbon coated grids (Quantifoil 300 mesh, Au R1.2/1.3), adsorbed for 10 s, blotted for 2 s at 100% humidity at 4 °C and plunge frozen in liquid ethane using a Vitrobot Mark IV (FEI).
Data were collected in counting mode on a Titan Krios G3 (FEI) operating at 300 kV with a GIF energy filter (Gatan) and K2 Summit detector (Gatan) using a pixel size of 0.822 Å and a total dose of 48 e−/Å2 spread over 20 fractions.
James R.H., Deme J.C., Kjær A., Alcock F., Silale A., Lauber F., Johnson S., Berks B.C, & Lea S.M. (2021). Structure and mechanism of the proton-driven motor that powers Type 9 secretion and gliding motility. Nature microbiology, 6(2), 221-233.
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4

Cryo-EM Sample Preparation and Imaging

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Aliquots (4 μL) of purified samples at an A280 of 1 were applied onto glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3), then adsorbed for 10 s, blotted for 2 s at 100% humidity at 4°C, and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI). To prepare samples with fluorinated octyl maltoside (fOM; Anatrace), proteins were concentrated to an A280 of 3 and 13.5 μL was mixed with 1.5 μL of 7 mM fOM in buffer W (100 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA) plus 0.01% LMNG. All samples were centrifuged at 18,400 × g for 10 min at 4°C immediately before grid preparation.
Data were collected using a Titan Krios G3 instrument (FEI) operated at 300 kV and fitted with either a GIF energy filter (Gatan) and a K2 Summit detector (Gatan) or a BioQuantum imaging filter (Gatan) and a K3 direct detection camera (Gatan). Full details of the data collection strategy are given in Text S1.
Hennell James R., Deme J.C., Hunter A., Berks B.C, & Lea S.M. (2022). Structures of the Type IX Secretion/Gliding Motility Motor from across the Phylum Bacteroidetes. mBio, 13(3), e00267-22.
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5

Cryo-EM Structure Determination of GldLM′ and PorLM

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Four microlitre aliquots of purified GldLM′ (A280nm = 0.4-0.5) or PorLM (A280nm = 0.2-0.5) complexes were applied onto glow-discharged holey carbon coated grids (Quantifoil 300 mesh, Au R1.2/1.3), adsorbed for 10 s, blotted for 2 s at 100% humidity at 4 °C and plunge frozen in liquid ethane using a Vitrobot Mark IV (FEI).
Data were collected in counting mode on a Titan Krios G3 (FEI) operating at 300 kV with a GIF energy filter (Gatan) and K2 Summit detector (Gatan) using a pixel size of 0.822 Å and a total dose of 48 e−/Å2 spread over 20 fractions.
James R.H., Deme J.C., Kjær A., Alcock F., Silale A., Lauber F., Johnson S., Berks B.C, & Lea S.M. (2021). Structure and mechanism of the proton-driven motor that powers Type 9 secretion and gliding motility. Nature microbiology, 6(2), 221-233.
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6

Cryo-EM Imaging of Membrane Protein

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Purified complex (3 μl) at 1–3.6 mg/ml were applied to glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3). Grids were blotted for 3 s at 100% humidity at 22 °C and frozen in liquid ethane using a Vitrobot Mark IV (FEI). For samples solubilized in detergent, blotting was preceded by a wait time of 5–10 s. V. mimicus FliPQR was supplemented with 0 mM, 0.05 mM, 0.5 mM or 3 mM fluorinated Fos-Choline prior to grid preparation.
Kuhlen L., Johnson S., Zeitler A., Bäurle S., Deme J.C., Caesar J.J., Debo R., Fisher J., Wagner S, & Lea S.M. (2020). The substrate specificity switch FlhB assembles onto the export gate to regulate type three secretion. Nature Communications, 11, 1296.
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7

Cryo-EM Sample Preparation for Vag8:C1-INH

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Four microliters of purified Vag8:C1-INH complex (0.5 mg/ml) was adsorbed to glowdischarged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3) for 10 s. Grids were then blotted for 3 s at 100% humidity at 8°C and frozen in liquid ethane using a Vitrobot Mark IV (FEI).
Dhillon A., Deme J.C., Furlong E., Roem D., Jongerius I., Johnson S., & Lea S.M. (2020). Molecular basis forB. pertussisinterference with complement, coagulation, fibrinolytic and contact activation systems: The cryo-EM structure of the Vag8-C1 inhibitor complex.
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8

Cryo-EM Sample Preparation using Fluorinated Octyl Maltoside

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4 µl aliquots of purified samples at A280 = 1 were applied onto glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3), then adsorbed for 10 s, blotted for 2 s at 100% humidity at 4 °C and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI). To prepare samples with fluorinated octyl maltoside (fOM; Anatrace), proteins were concentrated to A280 = 3 and 13.5 µl was mixed with 1.5 µl of 7 mM fOM in Buffer W + 0.01% LMNG. All samples were centrifuged at 18,400g for 10 min at 4 °C immediately before grid preparation. Data were collected using a Titan Krios G3 (FEI) operated at 300 kV fitted with either a GIF energy filter (Gatan) and a K2 Summit detector (Gatan) or a BioQuantum imaging filter (Gatan) and a K3 direct detection camera (Gatan). Full details of the data collection strategy are given in the Supplemental Methods.
James R.H., Deme J.C., Hunter A., Berks B.C., & Lea S.M. (2022). Structures of the Type IX Secretion/gliding motility motor from across the phylum Bacteroidetes.
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