The U-2001 spectrophotometer for adjusting the McFarland standard was from Hitachi (Chiyoda, Japan). For measurement of the 96-well plates, a Tecan Sunrise Remote Plate Reader (Tecan, Männedorf, Switzerland) was used. The adjustment of pH values was carried out with the pH-meter Mettler Toledo SevenMulti (Mettler-Toledo GmbH, Vienna, Austria).
Potato dextrose agar (pda)
The PDA is a portable digital assistant designed for use in laboratory environments. It provides a compact and convenient platform for data collection, analysis, and process monitoring. The core function of the PDA is to enable efficient data management and communication within the laboratory setting.
Lab products found in correlation
22 protocols using potato dextrose agar (pda)
Antimicrobial Potential of Natural Compounds
The U-2001 spectrophotometer for adjusting the McFarland standard was from Hitachi (Chiyoda, Japan). For measurement of the 96-well plates, a Tecan Sunrise Remote Plate Reader (Tecan, Männedorf, Switzerland) was used. The adjustment of pH values was carried out with the pH-meter Mettler Toledo SevenMulti (Mettler-Toledo GmbH, Vienna, Austria).
Antimicrobial Susceptibility Testing Protocol
Fungal Strains Cultivation and Analysis
Monascus Cultivation on Rice Substrate
An amount of rice (Giana, Thailand) (150 g) was washed with hot water, then boiled for 1 min. The rice was evenly divided into three autoclavable plastic bags, which were closed with a metal ring and a cotton plug. The use of plastic bags instead of glass vessels enabled manual separation of rice kernels after sterilization without opening of the bags. The cultivation was inspired by soy koji preparation (Lotong and Suwanarit, 1983 (link)). The bags were placed in a beaker sealed with aluminum foil and sterilized at 121°C for 20 min. Sterilization was repeated after 24 h to eliminate contamination by spore-forming bacteria. Spores from the Monascus culture (mixture of ascospores and conidia, because the strain formed both asexual and sexual spores, see
Preparation of Physiological Solution
Isolating and Characterizing Soil Fungi
Monascus Fungus Cultivation on Sterilized Rice
Impact of 1-octen-3-ol on ECM Fungal Growth
The solution of 1-octen-3-ol at 1 µM was prepared using 1-octen-3-ol 98% (Alfa Aesar CAS 3391-86-4) diluted in Chloroform 99% (Merck CAS 67-66-3) and sterilized by filtration (0.2 μm, 47 mm membrane filter, Minisart® NML, Sartorius, Gottingen, Germany). The mycelial plugs with 0.25 cm2 of T. leptoderma, L. deliciosus and B. reticulatus were transferred from 1-month pure cultures (in PDA) and placed in the centre of the Petri dishes of each culture medium. Each treatment was replicated 10 times (N = 10 Petri dishes).
The ECM fungal cultures were placed in a growth chamber at 23 °C ± 1 °C in the dark. Colonies’ growths were measured every 7 days for 63 days by measuring mycelia radial growth at the bottom of the Petri dish.
Fungal Morphology and Colony Characteristics
Antifungal Activity Evaluation of Talaromyces Extracts
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