Sk mel 28

B16F10 murine and SK-MEL-28 human melanoma cell lineswere purchased from the Korean Cell Line Bank (Seoul, Korea). The cells were cultured in Dulbecco's modified Eagle's medium (B16F10 cells) or modified Eagle's medium (SK-MEL-29 cells, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Atlas Biologicals, For Collins, Co, USA), 100 U/ml penicillin and 100 μg streptomycin (Sigma-Aldrich). Human umbilical vein endothelial cells (HUVECs) and their growth medium (Endothelial Cell Growth Medium) were purchased from PromoCell GmbH (Heidelberg, Germany). The cells were used at passage 4-5 in all experiments. All cells were incubated at 37 °C with 5% CO₂. Naringenin was dissolved in dimethyl sulfoxide to obtain a 200 μM stock solution, which was then diluted with media.

B16F10, A375SM and SK-MEL-28 melanoma cell lines were obtained from the Korean Cell Line Bank (KCLB). All cells were grown in DMEM supplemented with 10% FBS and maintained at 37 °C in a humidified 5% CO₂ incubator. Cell growth was examined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. The cells were seeded in 96-well culture plates at a density of 2 × 10³ cells/well. After 24 h incubation, various concentrations of BV and melittin were added to each well. After 72 h, 50 µL of MTT solution (2 mg/mL; Sigma-Aldrich) was added to each well, and the cells were incubated for 3 h. To dissolve formazan crystals, the culture medium was removed and an equal volume of DMSO was added to each well. The absorbance of each well was determined at a wavelength of 540 nm using a microplate reader (Thermo Fisher Scientific, Vantaa, Finland).

B16F10 (mouse melanoma cell line), SK-MEL-2 (human melanoma cell line), SK-MEL-28 (human melanoma cell line), UACC-257 (human melanoma cell line), and HEK293 (human embryonic kidney cell line) cells were cultured in RPMI (Welgene, Gyeongsan, South Korea) with 10% fetal bovine serum (FBS, RMBIO, Missoula, MT, USA) and 1% antibiotic-antimycotic (Gibco, Grand Island, NY, USA). All cells were maintained at 37 °C with 5% CO₂ in a humidified chamber. UACC-257 was provided by the Chungnam National University Hospital (Daejeon, South Korea). B16F10, HEK293, SK-MEL-2, and SK-MEL-28 cells were purchased from the Korean Cell Line Bank (KCLB, Seoul, South Korea).

A431 and SK-MEL-28 human skin cancer cells were obtained from the Korean Cell Line Bank (Seoul, Korea). A431 cells were grown in RPMI1640 supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin. SK-MEL-28 cells were grown in MEM supplemented with 10% fetal bovine serum, 1 mM sodium pyruvate, 1× MEM non-essential amino acid, 100 U/mL penicillin, and 100 μg/mL streptomycin. Cells were cultured at 37 °C in 5% CO₂ humidified air.

The malignant melanoma cell lines SK-MEL-28 (KCLB No. 30072) and G361 (KCLB No. 21424) were obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea). A non-tumoral immortalized human epidermal cell line,HaCaT, was provided from Mokpo University (Muan-gun). Cells were grown routinely in Dulbecco’s Modified Eagle’s Medium (DMEM; Biowest, Pays De La Loire, France) with 10% fetal bovine serum (FBS) and 100 U/mL each of penicillin and streptomycin (Gibco, Grand Island, NY, USA) in appropriate concentrations at 37 °C with 5% CO₂ in a fully humidified atmosphere. Cell culture was performed as previously described^{50 (link)}.