Cumulative Si release from the scaffolds was measured at 6, 12, 18, and 24 days. PN, PNM, and PNNM scaffold samples (20 mg) were incubated in 10 mL of distilled deionized water. The Si concentrations of each group were quantified through inductively coupled plasma–atomic emission spectroscopy (ICP–AES, Thermo Fisher Scientific Inc). Cumulative NAC release was detected through ultraviolet spectrophotometry at 24, 72, 120, 144, 168, 216, and 264 hours. PNNM, PNM, and PN scaffold samples (20 mg) were immersed in 2 mL of distilled deionized water. Then, the samples were mixed with 4-chloro-7-nitrobenzofurazan (0.006 wt%) chromogenic agent at the ratio of 1:18. After 30 minutes of reaction, NAC concentrations were calculated on the basis of the absorbance of the solution at 423 nm.
Icp aes
Manufactured by Thermo Fisher Scientific
Sourced in United States, Japan, Germany
The ICP-AES (Inductively Coupled Plasma Atomic Emission Spectrometry) is a type of analytical instrument used for the detection and quantification of trace elements in various samples. It utilizes the principle of atomic emission spectroscopy to identify and measure the concentrations of elements present in the sample.
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Lab products found in correlation
Dismic 25hp, by Advantec (2 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Escalab 250xi, by Thermo Fisher Scientific (2 mentions)
Axis ultra dld, by Shimadzu (1 mentions)
Jem 2100f, by JEOL (1 mentions)
D8 advance, by Bruker (1 mentions)
S 4300, by Hitachi (1 mentions)
X series 2, by Thermo Fisher Scientific (1 mentions)
Nano zs90, by Malvern Panalytical (1 mentions)
Tecnai g2 spirit, by Thermo Fisher Scientific (1 mentions)
Cary 5000, by Agilent Technologies (1 mentions)
Icp ms, by Thermo Fisher Scientific (1 mentions)
Tecnai f20, by Thermo Fisher Scientific (1 mentions)
Evolution 300, by Thermo Fisher Scientific (1 mentions)
Spectrum 100, by PerkinElmer (1 mentions)
Conductivity meter, by Thermo Fisher Scientific (1 mentions)
Organic carbon analyzer, by Analytik Jena (1 mentions)
Ion chromatography, by Thermo Fisher Scientific (1 mentions)
Ph electrode, by Thermo Fisher Scientific (1 mentions)
Elemental analyzer, by Elementar (1 mentions)
29 protocols using icp aes
1
Quantifying Si and NAC Release from Scaffolds
2
Measuring Metal Ion Content in Plants
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To measure metal ion content, about 500 mg of shoots and roots were firstly separated from Chrysanthemum plants, respectively. Then, the dried samples were ground and digested with 15 ml nitric acid (HNO3)/perhydrol (H2O2) (3:1, v/v) in a microwave system (MARS, CEM) at 160°C for 20 min. After centrifugation at 12,000 rpm for 10 min, the supernatant was used for determining metal ion contents by inductively coupled plasma-atomic emission spectroscopy (ICP-AES, Thermo Scientific, Waltham, MA, United States) as described recently by Lei et al. (2014) (link).
3
Characterization of W18O49 NCs-rGO Nanocomposite
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The crystalline
phases of the W18O49 NCs–rGO support
and the Pt/W18O49 NCs–rGO nanocomposite
were characterized by X-ray powder diffraction (XRD) (D8 Advance,
Bruker, Deutschland). Raman spectra were measured with a micro-Raman
spectrometer (HR 800, Jobin Yvon, France) at 457.9 nm wavelength.
X-ray photoelectron spectroscopy (XPS) was performed with Al Kα
radiation (Kratos AXIS Ultra DLD, Britain). The thermal analysis was
measured by thermogravimeter (TG/differential thermal analysis (DTA),
6300, SEIKO, Tokyo, Japan). Scanning electron microscopy (SEM) was
performed on a scanning electron microscope (Hitachi S-4300, Japan).
Transmission electron microscopy (TEM) and high-resolution transmission
electron microscopy (HRTEM) were performed on a microscope (JEOL JEM2100F,
Japan) operated at 200 keV. The compositions of the catalysts were
analyzed by inductively coupled plasma atomic emission spectrometry
(ICP-AES, Thermo Fisher).
phases of the W18O49 NCs–rGO support
and the Pt/W18O49 NCs–rGO nanocomposite
were characterized by X-ray powder diffraction (XRD) (D8 Advance,
Bruker, Deutschland). Raman spectra were measured with a micro-Raman
spectrometer (HR 800, Jobin Yvon, France) at 457.9 nm wavelength.
X-ray photoelectron spectroscopy (XPS) was performed with Al Kα
radiation (Kratos AXIS Ultra DLD, Britain). The thermal analysis was
measured by thermogravimeter (TG/differential thermal analysis (DTA),
6300, SEIKO, Tokyo, Japan). Scanning electron microscopy (SEM) was
performed on a scanning electron microscope (Hitachi S-4300, Japan).
Transmission electron microscopy (TEM) and high-resolution transmission
electron microscopy (HRTEM) were performed on a microscope (JEOL JEM2100F,
Japan) operated at 200 keV. The compositions of the catalysts were
analyzed by inductively coupled plasma atomic emission spectrometry
(ICP-AES, Thermo Fisher).
4
Cadmium Removal by Algal Biomass
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10 mL of filtrate from each trial of Plackett-Burman and FCCD were taken, filtered by double rings filter paper (Qualitative medium speed 102, diameter 12.5 cm) and analyzed using inductively coupled plasma – atomic emission spectroscopy (ICP-AES, Thermo Scientific).
The efficiency of alga biomass for cadmium ions removal from aqueous solutions was quantitatively calculated as follows: Where: Ci is the initial metal ion concentration (mg/L), Cf is the final (residual) metal ion concentration (mg/L). All Determinations of cadmium ions in the solution were carried out in triplicates.
The efficiency of alga biomass for cadmium ions removal from aqueous solutions was quantitatively calculated as follows: Where: Ci is the initial metal ion concentration (mg/L), Cf is the final (residual) metal ion concentration (mg/L). All Determinations of cadmium ions in the solution were carried out in triplicates.
5
Elemental Analysis of IDC and Iohexol
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Elemental analysis of IDC and iohexol were conducted with energy dispersive X-ray spectroscopy (EDX S-10, Oxford instrument, Abingdon, UK) at 15 kV accelerating voltage. Additionally, the iodine contents in the IDC and iohexol were calculated based on the iodide standard solution using inductively coupled plasma atomic emission spectroscopy (ICP–AES, Thermo Fisher Scientific, Bremen, Germany).
6
Quantifying Phosphorus Content in Plants
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Leaf and root samples exposed to Pi-sufficient and Pi-deficient media were harvested and dried at 80 °C to constant weight. A mixture of 50 mg of dry material, 5 ml of nitric acid, and 2 ml of hydrogen peroxide was placed in digestion tubes, and then samples were digested using a microwave system (MARS, CEM). After diluting and filtering, P concentrations were measured using an ICP-AES (Thermo).
7
Bioactive Glass Hydrogel Release Kinetics
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First, 1 g of hydrogel was added to 10 mL of phosphate-buffered saline (PBS) buffer solution and incubated for different periods (1st, 2nd, 3rd, 4th, 7th, 14th, 21st, and 28th day). After incubation, the suspension was centrifuged at 4000 rpm for 10 min at a pre-set time. The release medium was collected and replaced with 10 mL of fresh PBS solution. The collected release medium was analyzed using the concentration of Sr2+ and SiO32− (ICP-AES; Thermo Fisher X Series 2, USA).
8
Biomass-based Pb2+ Removal Efficiency
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10 mL of filtrate from each trial filtered through disposable 0.2 µm PTFE syringe filters (DISMIC-25HP, Advantec, Tokyo, Japan) and analyzed using inductively coupled plasma – atomic emission spectroscopy (ICP-AES, Thermo Scientific. The efficiency of Gelidium amansii biomass for Pb2+ ions removal from aqueous solutions was calculated quantitatively by using the following equation: where: Ci is the initial metal ion concentration (mg/L), Cf is the final (residual) metal ion concentration (mg/L). All determinations of Pb2+ ions in the solution were carried out in triplicates.
10
Elemental Analysis of PM2.5
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Chemical components were detected based on inductively coupled plasma mass spectrometry (ICP-MS; Thermo Fisher, NJ, USA) and inductively coupled plasma-atomic emission spectrometry (ICP-AES; Thermo Fisher, NJ, USA), respectively. Ion chromatography (IC) was used to measure the cations (Mg2+, Ca2+, K+, Na+ and NH4+) and the anions (SO42−, NO2− and Cl−) contained in PM2.5. The details of the method were reported as previously described [15 (link)].
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