Mouse monoclonal anti lsr antibody

A rabbit polyclonal anti-actin antibody, EGFR inhibitor (AG1478) and 2-deoxy-D-glucose (2-DG) were obtained from Sigma-Aldrich (St. Louis, Mo., USA). Dobutamine hydrochloride was obtained from Wako Laboratory Chemicals (Tokyo, Japan). Recombinant human amphiregulin was obtained from PeproTech (Rocky Hill, NJ). Rabbit polyclonal anti-lipolysis-stimulated receptor (LSR) and anti-tricellulin (TRIC) antibodies were obtained from Zymed Laboratories (San Francisco, CA). A mouse monoclonal anti-LSR antibody was obtained from Abnova (Yaipei, Taiwan). A rabbit polyclonal anti-angiomotin (AMOT) antibody was obtained from Bioss, Inc. (Woburn, MA). Rabbit monoclonal anti-Merlin, anti-phospho- YAP (ser127) and anti-phospho-AMPK (ther172) antibodies were obtained from Cell Signaling Technology Japan (Tokyo, Japan). A rabbit polyclonal anti-YAP antibody was obtained from Novus Biologicals (Littleton, CO). Alexa 488 (green)-conjugated anti-rabbit IgG and Alexa 594 (red)-conjugated anti-mouse IgG antibodies were purchased from Molecular Probes, Inc. (Eugene, OR).

Rabbit polyclonal anti-tricellulin (TRIC), anti-claudin (CLDN)-1 and -2 antibodies and mouse monoclonal CLDN-2 and anti-occludin (OCLN) antibodies were obtained from Zymed Laboratories (San Francisco, CA, USA). Rabbit polyclonal anti-lipolysis-stimulated lipoprotein receptor (LSR) antibodies were from Novus Biologicals (Littleton, CO, USA). A mouse monoclonal anti-LSR antibody was obtained from Abnova (Taipei, Taiwan). A rabbit polyclonal anti-p63 antibody was obtained from Abcam (Cambridge, UK). Mouse monoclonal anti-acetylated tubulin and antiCK7 antibodies and a rabbit polyclonal anti-actin antibody were from Sigma-Aldrich (St. Louis, MO, USA). Alexa 488 (green)-conjugated anti-rabbit IgG and Alexa 594 (red)-conjugated anti-mouse IgG antibodies and Alexa 594 (red)-conjugated phalloidin were from Molecular Probes, Inc. (Eugene, OR, USA). HRP-conjugated polyclonal goat anti-rabbit IgG was from Dako A/S (Glostrup, Denmark). The ECL Western blotting system was from GE Healthcare UK, Ltd. (Buckinghamshire, UK). A rabbit polyclonal anti-actin antibody and EGFR inhibitor (AG1478) were obtained from Sigma-Aldrich (St. Louis, MO, USA). A TGF-β receptor type 1 inhibitor (EW-7197) was obtained from Cayman Chemical (Ann Arbor, MI, USA). FITC-dextran (FD-4, MW 4.0 kDa) was obtained from Sigma-Aldrich Co. (St. Louis, MO, USA).