Cat selective supplement

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom

The CAT selective supplement is a laboratory product designed to facilitate the growth and isolation of Campylobacter species from clinical and environmental samples. It provides the necessary nutrients and selective agents to suppress the growth of competing microorganisms, allowing for the effective detection and identification of Campylobacter.

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Lab products found in correlation

Tryptone soy broth (tsb), by Thermo Fisher Scientific (1 mentions) Campylobacter blood free selective agar base, by Thermo Fisher Scientific (1 mentions) Xld agar, by Thermo Fisher Scientific (1 mentions) Ec broth, by Thermo Fisher Scientific (1 mentions) Rappaport vassiliadis broth, by Thermo Fisher Scientific (1 mentions) Macconkey agar, by Thermo Fisher Scientific (1 mentions)

2 protocols using cat selective supplement

Isolation of Campylobacter spp. from Feces

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The isolation of Campylobacter spp. from each fecal sample was carried out with an enrichment step in tubes containing Tryptone Soy Broth (Oxoid Ltd., Basingstoke, UK) liquid medium added with 5% horse blood and incubated at 37 °C for 4 h, then at 42 °C for 24 h.
A loop of each broth culture was streaked onto dishes containing Campylobacter Blood-Free selective agar base added with CAT selective supplement (Oxoid Ltd., Basingstoke, UK). Plates were incubated at 37 °C for 48 h in microaerophilia (5% oxygen, 10% CO₂) [22 (link)].

Ebani V.V., Nardoni S., Ciapetti S., Guardone L., Loretti E, & Mancianti F. (2021). Survey on the Presence of Bacterial, Fungal and Helminthic Agents in Off-Leash Dog Parks Located in Urban Areas in Central-Italy. Animals : an Open Access Journal from MDPI, 11(6), 1685.

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Isolation and Detection of Foodborne Pathogens from Eggshells

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The surface of each eggshell was swabbed with a sterile cotton stick. The swabbed cotton sticks were vortexed and suspended in 0.1% (w/v) peptone water. E.coli and Salmonella species were isolated using the standard protocols of the food code established by the Ministry of Food and Drug Safety (MFDS) in Korea. Briefly, EC broth (Oxoid) and MacConkey agar (Oxoid) were used to isolate E. coli. Rappaport Vassiliadis broth (Oxoid) and XLD agar were used to isolate Salmonella species.
Arcobacter species were isolated as described by Lee et al. (2010) (link). Each sample was mixed with Arcobacter-selective broth (Oxoid) including a cefoperazone, amphotericin, and teicoplanin (CAT)-selective supplement (Oxoid) at a 1:10 ratio and incubated in microaerobic conditions at 37℃ for 48 h. The enriched broth was inoculated and incubated in Arcobacter-selective agar (Oxoid) at 37℃ for 48 h. To detect Arcobacter species, species-specific multiplex polymerase chain reaction (PCR) was performed with boiled enriched broth, as described previously (Lee et al., 2010 (link)).

Lee M., Seo D.J., Jeon S.B., Ok H.E., Jung H., Choi C, & Chun H.S. (2016). Detection of Foodborne Pathogens and Mycotoxins in Eggs and Chicken Feeds from Farms to Retail Markets. Korean Journal for Food Science of Animal Resources, 36(4), 463-468.

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