DUCaP or LNCaP cells were steroid starved for 3 days before treatment with 1 nM R1881 or equivalent ethanol for 8 or 24 h. Total RNA was isolated using TRI reagent (Sigma, St. Louis, Missouri) according to the manufacturer’s instructions. Biological triplicate (24 h treatment) and duplicate (8 h treatment) RNAs were hybridized to Affymetrix HuGene-1.0 st v1 expression arrays (Affymetrix, Santa Clara, CA) (Irizarry, et al., 2003 (link)) in the Core Facility of the Medical University of Innsbruck. Raw microarray data was pre-processed in R (http://www.r-project.org ) using the RMA algorithm and the custom CDF HuGene10stvs1_Hs_ENSG version 12 (Smyth, 2004 (link)). Raw and pre-processed microarray data have been deposited to the Gene Expression Omnibus (accession Nr. GSE63693). Significance for differential expression was estimated using the moderated t-test and the resulting p-values were adjusted for multiple hypothesis testing using the Benjamini and Hochberg correction method (Benjamini and Hochberg, 1995 ). Probe sets with a B-H corrected p-value ≤0.05 were considered to represent androgen regulated genes.
Hugene 1.0 st v1 expression arrays
Manufactured by Thermo Fisher Scientific
The HuGene-1.0 st v1 expression arrays are high-density oligonucleotide microarrays designed for gene expression analysis. The arrays provide comprehensive coverage of the human transcriptome, allowing for the measurement of gene expression levels across a wide range of genes.
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Lab products found in correlation
2 protocols using hugene 1.0 st v1 expression arrays
1
Androgen-Regulated Gene Expression in Prostate Cancer Cells
2
Androgen-Regulated Gene Expression
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DUCaP or LNCaP cells were steroid starved for 3 days before treatment with 1 nM R1881 or equivalent ethanol for 8 or 24 hr. Total RNA was isolated using TRI reagent (Sigma, St. Louis, MO) according to the manufacturer's instructions. Biological triplicate (24‐hr treatment) and duplicate (8‐h treatment) RNAs were hybridized to Affymetrix HuGene‐1.0 st v1 expression arrays (Affymetrix, Santa Clara, CA) [Irizarry et al., 2003 ] in the Core Facility of the Medical University of Innsbruck. Raw microarray data were preprocessed in R (http://www.r‐project.org ) using the RMA algorithm and the custom CDF HuGene10stvs1_Hs_ENSG version 12 [Smyth, 2004 ]. Raw and preprocessed microarray data have been deposited to the Gene Expression Omnibus (accession Nr. GSE63693). Significance for differential expression was estimated using the moderated t‐test and the resulting P values were adjusted for multiple hypotheses testing using the Benjamini and Hochberg correction method [Benjamini and Hochberg, 1995 ]. Probe sets with a B‐H‐corrected P value ≤ 0.05 were considered to represent androgen‐regulated genes.
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