Lct premier mass spectrometer

Manufactured by Waters Corporation

Sourced in United States

The LCT Premier mass spectrometer is a high-performance liquid chromatography (LC) instrument designed for accurate mass measurement and molecular characterization. It utilizes time-of-flight (TOF) technology to provide precise mass detection and identification of compounds.

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Lab products found in correlation

Nicolet is5 ftir spectrometer, by Thermo Fisher Scientific (7 mentions) Pre coated glass plates, by Merck Group (7 mentions) Xl 400 spectrometer, by Agilent Technologies (2 mentions) Synergy mx microplate reader, by Agilent Technologies (2 mentions) Waters acquity uplc system, by Waters Corporation (2 mentions) Avance 400, by Bruker (2 mentions) Waters 2998 uv detector, by Waters Corporation (1 mentions) Avance 3 400 mhz, by Brucker (1 mentions) Hplc system, by Waters Corporation (1 mentions) Exactive esi orbitrap mass spectrometer, by Thermo Fisher Scientific (1 mentions) Magnesium ribbon, by Merck Group (1 mentions) Copper 1 iodide, by Merck Group (1 mentions) Phosphorus trichloride, by Merck Group (1 mentions) Labmaster dp, by MBraun (1 mentions) Avance dpx 400 mhz spectrometer, by Bruker (1 mentions) Lithium bromide, by Merck Group (1 mentions) Aeris widepore xb c18 column, by Phenomenex (1 mentions) Pngase f, by Roche (1 mentions) Obeticholic acid, by Merck Group (1 mentions) Acquity uplc system, by Waters Corporation (1 mentions)

Close spelling variants of this product

LCT Premier time of flight mass spectrometer LCT-Premier TOF mass spectrometer Micromass LCT Premier Mass Spectrometer LCT Premier XE mass spectrometer LCT Premier XE ESI-TOF mass spectrometer LC-TOF mass spectrometer (model LCT-XE Premier) Micromass LCT Premier XE Mass Spectrometer Micromass-LCT Premier Time of Flight (TOF) mass spectrometer LCT premier XE time-of-flight mass spectrometer LCT Premier orthogonal accelerated time of flight mass spectrometer

27 protocols using lct premier mass spectrometer

Characterization of Synthesized Compounds

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Commercially available chemicals were purchased from Fujifilm Wako Pure Chemical Co. (Osaka, Japan), unless otherwise stated. Absorbance was measured using a Synergy™ MX microplate reader (BioTek Instruments, Inc., Winooski, VT, USA). A Bruker AMX 500 instrument (Bruker BioSpin K.K., Bruker Instruments, Billerica, MA, USA) was used to obtain nuclear magnetic resonance (NMR) spectra and residual solvents were used as internal standards (pyridine-d5: 1 H 7.22 ppm, 13 C 135.91 ppm).
Mass spectra were obtained using an LCT-Premier mass spectrometer (Waters Corp., Milford, MA, USA) or a Jeol JMS-T100GCV (Jeol Ltd., Tokyo, Japan). For ultra-high-performance liquid chromatography (UPLC)-mass spectrometry (MS) analysis, a Waters Acquity UPLC system (Waters Corp., Milford, MA, USA) was linked to an LCT-Premier mass spectrometer.

Lahrita L., Moriai K., Iwata R., Itoh K, & Kato E. (2019). Quassinoids in Brucea javanica are potent stimulators of lipolysis in adipocytes. Fitoterapia, 137.

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Isolation and Characterization of Bioactive Compounds from Eurycoma longifolia

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Unless otherwise stated, commercially available chemicals were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). E. longifolia root (Batch No. SL.1A.2015.PB) was supplied by Merapi Farma Herbal Co. (Yogyakarta, Indonesia).
Absorbance was measured using a Synergy™ MX microplate reader (BioTek Instruments, Inc., Winooski, VT, USA). A Bruker AMX 500 instrument (Bruker BioSpin K.K., Bruker Instruments, Billerica, MA, USA) was used to obtain NMR spectra and residual solvents were used as an internal standard (pyridine-d 5 : 1 H 7.22 ppm, 13 C 135.91 ppm). Mass spectra were obtained using a LCT-Premier mass spectrometer (Waters Corp., Milford, MA, USA). For the LC-MS analysis, Waters Acquity UPLC system (Waters Corp., Milford, MA, USA) was combined with LCT-Premier mass spectrometer (Waters Corp., Milford, MA, USA). The EZR was used for the statistical tests. 22

Lahrita L., Hirosawa R., Kato E, & Kawabata J. (2017). Isolation and lipolytic activity of eurycomanone and its epoxy derivative from Eurycoma longifolia. Bioorganic & medicinal chemistry, 25(17).

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Synthesis and Characterization of Novel Compounds

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Reagents and dry solvents were purchased from various chemical suppliers (Aldrich, Acros Organics, ChemImpex, TCI America, and Bachem) and were used as obtained. Silica gel (230–450 mesh) used for flash chromatography was purchased from Sorbent Technologies (Atlanta, GA). Thin layer chromatography was performed using Analtech silica gel plates. Visualization was accomplished using UV light and/or iodine. The ¹H NMR were recorded in CDCl₃ or DMSO-d₆ using a Varian XL-400 spectrometer and are reported relative to TMS (δ_H = 0.00 ppm). Chemical shifts are reported in ppm and spin multiplicities are represented by the following signals: s (singlet), d (doublet), dd (doublet of doublets), t (triplet), q (quartet) and m (multiplet). Melting points were recorded on a Mel-Temp apparatus and are uncorrected. High resolution mass spectrometry (HRMS) was performed at the University of Kansas Mass Spectrometry lab using an LCT Premier mass spectrometer (Waters, Milford, MA) equipped with a time of flight mass analyzer and an electrospray ion source. The purity of the compounds was established using HPLC and was >95%.

Weerawarna P.M., Kim Y., Galasiti Kankanamalage A.C., Damalanka V.C., Lushington G.H., Alliston K.R., Mehzabeen N., Battaile K.P., Lovell S., Chang K.O, & Groutas W.C. (2016). Structure-based design and synthesis of triazole-based macrocyclic inhibitors of norovirus protease: Structural, biochemical, spectroscopic, and antiviral studies. European Journal of Medicinal Chemistry, 119, 300-318.

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Mass Spectrometry of Organic Samples

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We collected the mass spectra using both laser desorption ionization (LDI) and electrospray ionization (ESI) mass spectroscopy. ESI mass spectroscopy was performed on a Waters LCT Premier mass spectrometer. MassLynx was used as the software to collect spectra. Each ESI mass spectrum shown in this work was an average of 55 mass spectra collected in 1 minute with 0.1 second between every two one-second scans. Samples were diluted about 10 times before injection. For LDI measurements, we used the linear mode of the Comstock RTOF-210 mass spectrometer with a pulsed Nd:YAG laser at 355 nm (New Wave Research Polaris II). The laser power was less than 400 μJ/pulse. All the LDI mass spectra exhibited here were averaged from 200~500 scans. Sample solutions were applied to a solid copper tip, dried in air to form a thin film, and then inserted into the ion source.

Lee C., Liu X., Zhang W., Duncan M.A., Jiang F., Kim C., Yan X., Teng Y., Wang H., Jiang W., Li Z, & Xie J. (2021). Ultrasmall Gd@Cdots as a Radiosensitizing Agent for Non-Small Cell Lung Cancer. Nanoscale, 13(20), 9252-9263.

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Synthesis and Characterization of Organic Compounds

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Reagents and dry solvents were purchased from various chemical suppliers (Aldrich, Acros Organics, Chem-Impex, TCI America, and Bachem) and were used as obtained. Silica gel (230–450 mesh) used for flash chromatography was purchased from Sorbent Technologies (Atlanta, GA). Thin layer chromatography was performed using Analtech silica gel plates. Visualization was accomplished using UV light and/or iodine. NMR spectra were recorded in CDCl₃ or DMSO-d₆ using a Varian XL-400 spectrometer. Melting points were recorded on a Mel-Temp apparatus and are uncorrected. High resolution mass spectrometry (HRMS) was performed at the University of Kansas Mass Spectrometry lab using an LCT Premier mass spectrometer (Waters, Milford, MA) equipped with a time of flight mass analyzer and an electrospray ion source. The purity of the compounds was established using HPLC and was >95%.

Kankanamalage A.C., Kim Y., Weerawarna P.M., Uy R.A., Damalanka V.C., Mandadapu S.R., Alliston K.R., Mehzabeen N., Battaile K.P., Lovell S., Chang K.O, & Groutas W.C. (2015). Structure-Guided Design and Optimization of Dipeptidyl Inhibitors of Norovirus 3CL Protease. Structure-Activity Relationships and Biochemical, X-ray Crystallographic, Cell-Based, and In Vivo Studies. Journal of medicinal chemistry, 58(7), 3144-3155.

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Characterizing pABOL Reduction Byproducts

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The byproduct of pABOL reduction
was characterized using a Waters LCT Premier mass spectrometer. Samples
were ionized using electrospray ionization (ESI).

Blakney A.K., Zhu Y., McKay P.F., Bouton C.R., Yeow J., Tang J., Hu K., Samnuan K., Grigsby C.L., Shattock R.J, & Stevens M.M. (2020). Big Is Beautiful: Enhanced saRNA Delivery and Immunogenicity by a Higher Molecular Weight, Bioreducible, Cationic Polymer. ACS Nano, 14(5), 5711-5727.

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Spectroscopic Characterization of Compounds

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¹H and ¹³C NMR spectra were recorded at 25 °C on the Brucker Avance III 400 MHz. Unless otherwise specified, chemical shifts δ were expressed in parts per million (ppm) based on the residual solvent signal in DMSO-d₆ (δ 2.50 ppm) or tetramethylsilane (TMS) in CDCl₃ (δ 0.00 ppm), and coupling constants J are given in Hz. Coupling patterns are abbreviated as s (singlet), d (doublet), t (triplet), q (quartet), quin (quintet), ABq (AB quartet), and m (multiplet). Electrospray mass spectra were recorded on a Waters LCT Premier mass spectrometer in positive ion mode. Reverse phase HPLC was performed using Waters HPLC system equipped with Waters-1525 solvent delivery systems and Waters 2998 UV detector. Separation was achieved on a semi-preparative Phenomenex (Torrance, CA) Jupiter® 5μm C18 300Å column (5 micron, 250 × 10.00 mm, Part Number: 00G-4053-N0) at a flow rate maintained at 4.0 mL/min. A solvent system composed of water containing 0.1% TFA as solvent A and acetonitrile containing 0.1% TFA as solvent B was used over the stated gradient. The column effluent was monitored with a UV/Vis detector set at 760 nm.

Ling X., Zhang S., Shao P., Li W., Yang L., Ding Y., Xu C., Stella N, & Bai M. (2015). A novel near-infrared fluorescence imaging probe that preferentially binds to cannabinoid receptors CB2R over CB1R. Biomaterials, 57, 169-178.

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Native Mass Spectrometry of G-Quadruplexes

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All mass spectra were obtained in negative ion mode with injection concentrations of 5 μM in G-quadruplexes and 5 μM internal standard dT₆. Native electrospray mass spectra of oligonucleotides were obtained using several instruments, with similar results. (1) On an LCT Premier mass spectrometer (Waters, Manchester, UK), the ESI source voltage was set to 2.2 kV with a desolvation temperature of 60°C. Unless otherwise mentioned, the ion guide 1 voltage is 50 V. The source pressure was increased to 35 mbar. This pressure is measured using a Center Two probe (Oerlikon Leybold Vacuum, Cologne, Germany). The syringe injection flow was fixed to 200 μL/h. In all experiment 5 μM dT₆ (monoisotopic mass 1762.318 Da) was used as internal standard. (2) An Exactive ESI-Orbitrap mass spectrometer (Thermo Scientific, Bremen, Germany) was also used to monitor the titration of TG₄T by KCl. In this case, ESI spray voltage and capillary voltage are 2.75 kV and –20 V, respectively. Capillary temperature is set to 150°C. Tube lens and skimmer voltage are fixed to –180 V and –10 V. In order to help the desolvation process to occur, the HCD cell voltage is set to 10 eV and the cell pressure is 2.5 × 10^–5 mbar.

Marchand A, & Gabelica V. (2014). Native Electrospray Mass Spectrometry of DNA G-Quadruplexes in Potassium Solution. Journal of the American Society for Mass Spectrometry, 25(7), 1146-1154.

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Metal Complex Formation of Peptoid Oligomers

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Samples for MS analysis were prepared shortly before measurements. In a typical experiment, a solution of peptoid oligomers (100–200 μL of 0.05 mM) in MeOH or ACN was treated with metal solution (5 mM in H₂O or ACN) and the mixture was stirred for 30 minutes prior to MS analysis. Mass spectrometry analysis of the metal complexes was performed on a Waters LCT Premier mass spectrometer under electrospray ionization (ESI), direct probe ACN : H₂O (70 : 30), flow rate 0.3 mL min^–1.

Baskin M, & Maayan G. (2016). A rationally designed metal-binding helical peptoid for selective recognition processes †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5sc04358a. Chemical Science, 7(4), 2809-2820.

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Synthesis and Characterization of tert-Butyl Chloride

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Tert‐butyl chloride was synthesised at the Deuteration Facility at ISIS Neutron and Muon Source, STFC Rutherford Appleton Laboratory in Oxfordshire, UK. Phosphorus trichloride (99%), magnesium ribbon (>99.5%), copper(I) iodide (>99.5%) and lithium bromide (>99%) were purchased from Sigma Aldrich and used as received. The glovebox used in this work was MBraun Labmaster dp, operating typically under argon, at or below 0.6 ppm of H₂O and O₂. ¹³C and ³¹P NMR spectra were recorded on a Bruker Avance DPX 400 MHz spectrometer. Electrospray mass spectrometry measurements were performed using a Waters LCT Premier mass spectrometer.

Brown L.C., McGrogan A., Delavoux Y., Hogg J.M., Holbrey J.D., Gunaratne H.Q., Swadźba‐Kwaśny M., Tellam J.P, & Youngs S.E. (2022). The synthesis of deuteriated tri‐tert‐butyl phosphine. Journal of Labelled Compounds & Radiopharmaceuticals, 65(13), 338-342.

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