Alpha a5100

Manufactured by Sony

Sourced in Japan

The Sony Alpha A5100 is a compact, interchangeable-lens digital camera. It features a 24.3-megapixel APS-C CMOS sensor and the BIONZ X image processor. The camera is capable of recording full HD 1080p video at 60 fps. It has a 3-inch tilting touchscreen LCD and supports Wi-Fi and NFC connectivity.

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Lab products found in correlation

White led light, by Thorlabs (1 mentions)

5 protocols using alpha a5100

HyP-2 Paper-Based Fluorescent Sensor

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HyP-2 solution in DMSO (30 μM) was sprayed three times to a cellulose-based filter paper and dried at 25 °C. HyP-2 pre-treated paper was soaked in hydrazine solution (100 mM in deionized water) for 1 s and air-dried at 25 °C for 1 min. The fluorescence change of the strips was recorded using a digital camera (Sony, Alpha A5100, Tokyo, Japan) under UV light (365 nm).

Jung Y., Park N.K., Kang J.S, & Kim D. (2019). Hydrazine-Selective Fluorescent Turn-On Probe Based on Ortho-Methoxy-Methyl-Ether (o-MOM) Assisted Retro-aza-Henry Type Reaction. Sensors (Basel, Switzerland), 19(20), 4525.

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Soil-Based Fluorescent Sensing Assay

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A spoon of each soil (1 g, sand soil, clay soil, and field, respectively; Science Love, Republic of Korea) was transferred to an aluminum dish (Hanil, 52807, China). Two milliliters of DCNP solution (100 mM) in acetonitrile was poured into an aluminum dish under room temperature (25 °C). Soils were incubated for 2 min at 25 °C. After incubation, each soil sample was transferred into 3 mL of DMHN1 solution (10 μM) in CH₃CN (1% Et₃N). The fluorescence changes of the solution were investigated for 0–120 min by a digital camera (Sony, Alpha A5100, Tokyo, Japan) under UV light (365 nm). The relative fluorescence intensity and standard deviation were calculated by Image-J software (NIH, Bethesda, Rockville, MD, USA) in the specific fluorescence signal region.

Jung Y, & Kim D. (2019). A Selective Fluorescence Turn-On Probe for the Detection of DCNP (Nerve Agent Tabun Simulant). Materials, 12(18), 2943.

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UAV-Based Multispectral Mapping for Invasive Plant Detection

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For the purpose of this study, we designed a low-cost UAV capable of performing fully-automated mapping missions controlled by the APM ArduPlane/PixHawk autopilot. Its aerial segment is propelled by an electric BLDC motor, features a flying wing design and carries two modified consumer cameras (Sony Alpha A5100 with APS-C sensor and Sony E 20/2.8 lens). The first camera acquired standard RGB, and the second was modified to be sensitive in the NIR band by the removal of the built-in IR-cut filter and the addition of a Hoya R72 filter. This setup was used as a low-cost alternative to a more expensive multispectral camera (for more details on the UAV description see Müllerová et al., 2017 (link)). Using this UAV, we could test a suitable period in the seasonal development of the target species, focusing on times when it is possibly distinct from the background, such as, flowering for giant hogweed and autumn senescence for knotweeds.

Müllerová J., Brůna J., Bartaloš T., Dvořák P., Vítková M, & Pyšek P. (2017). Timing Is Important: Unmanned Aircraft vs. Satellite Imagery in Plant Invasion Monitoring. Frontiers in Plant Science, 8, 887.

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Retinal Imaging via Trans-palpebral Illumination

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This study was approved by the Institutional Review Board of the University of Illinois at Chicago and was in compliance with the Declaration of Helsinki. Figure 2 shows a schematic diagram of the trans-palpebral illumination imaging system. A custom made adaptor was attached to a Sony Alpha a5100 mirrorless digital camera. The adaptor consists of one ophthalmic lens (Volk 90D, Volk Optical Inc) and one relay lens (25D achromatic doublets, Thorlabs) housed in the tube system. The ophthalmic lens collects light coming from the eye and forms an aerial image between the ophthalmic and relay lenses. The aerial image is captured by the camera through the relay lens. The focusing system of the camera lens was set to the manual focusing mode. The photographs were captured when the optic disc and central retinal area were optimally focused. During the experiment, the eye was illuminated with a warm white LED light (Thorlabs Inc.) through the palpebra. A 1.5 mm diameter fiber was used to illuminate the palpebra. The field of view of the imaging system was calculated based on ISO 10940:2009 [12 ]. A target placed at 1 m from the imaging device was used to measure the corresponding external angle of the imaging system.

Toslak D., Thapa D., Chen Y., Erol M.K., Paul Chan R.V, & Yao X. (2017). Wide-field fundus imaging with trans-palpebral illumination. Proceedings of SPIE--the International Society for Optical Engineering, 10045, 100451X.

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Fluorescent sensing kit for DCNP detection

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DMHN1 solution (10 μM, CH₃CN (1% Et₃N), 1 mL) was placed in a screw-cap HPLC vial (2 mL size, YL Science, YL-VO1236, Guri, Korea) as a prototype sensing kit. A drop of the original DCNP solution was collected and transferred to the vial by using a capillary tube (Marienfeld, Non-heparinized, Lauda-Königshofen, Germany). The fluorescence changes of the kit were monitored for ~40 s with a digital camera (Sony, Alpha A5100, Tokyo, Japan) under UV light (365 nm). The relative intensity and standard deviation were calculated by Image-J software (NIH, Bethesda, Rockville, MD, USA) in the fluorescence signal region from video.

Jung Y, & Kim D. (2019). A Selective Fluorescence Turn-On Probe for the Detection of DCNP (Nerve Agent Tabun Simulant). Materials, 12(18), 2943.

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