Norepinephrine hydrochloride

³H-norepinephrine, 20 Ci/mmol, ³H-serotonin, 80 Ci/mmol, and ³H-dopamine, 60 Ci/mmol were purchased from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA). Dopamine hydrochloride, norepinephrine hydrochloride, serotonin hydrochloride, sodium L-ascorbate, paroxetine hydrochloride, venlafaxine hydrochloride, nisoxetine hydrochloride, GBR 12935 dihydrochloride, decynium-22 and hydrocortisone were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tri Reagent solution was obtained from the Molecular Research Centre (Cincinnati, OH, USA). Bicinchoninic acid assay (BCA assay) reagents were purchased from Thermo Fisher Scientific (Rockford, MA, USA). All other chemicals were of analytical grade.

All chemicals were used as received. Citrate-stabilized 80 nm AuNPs (optical density 1.0 at 555 nm) were purchased from BBI Solutions. Analytical-grade chloroform (≥ 99.8 %) was obtained from Merck. HCl (37%) and methylene blue hydrate (MB) (≥96%) were from Fisher Scientific. NaCl (≥ 99%), K₂HPO₄ (≥98%), and KH₂PO₄ (≥98%) were from Alfa Aesar. Cucurbit[5]uril hydrate (≈ 20% water), adenine (ADN, ≥99%), cytosine (CYT, ≥99%), 1,4-benzenedithiol (BDT, ≥99%), 2-naphthalenethiol (2-NT, ≥99%), 4-biphenylthiol (BPT, ≥97%), 4,4-biphenyldithiol (BPDT, ≥95%), 4-mercaptopyridine (4-MPY, ≥95%), hypoxanthine (HYP, ≥99.0%), creatinine (CR, ≥98%), nicotinamide (NAM, ≥98%), paracetamol (PAR, ≥99%), (±)-norepinephrine hydrochloride (NEPI, ≥97%), L-tryptophan (TRP, ≥98%), nicotinic acid (NA, ≥98%), and 96% ethanol (EtOH) were obtained from Sigma-Aldrich. Lyophilized pooled human urine controls (Level II for biogenic amines, Ref 8821, Lot 1066) were from ClinChek Controls. Polydimethylsiloxane (PDMS) was prepared using a SYLGARD 184 kit from DOWSIL (Dow Silicones). Fluorine-doped tin oxide (FTO)-coated glass slides (TEC 10) were purchased from Ossila Ltd and were cleaned and cut to 10 × 15 mm² slides prior to use. All aqueous solutions were prepared using deionized (DI) water (>18.2-MΩ cm⁻¹) from a Purelab Ultra Scientific water purification system.

Hydrogen tetrachloroaurate (HAuCl₄.3H₂O), silver nitrate (AgNO₃), sodium borohydride (NaBH₄), cetyltrimethylammonium bromide (CTAB), 5-bromosalicylic acid (5-Br-SA), ascorbic acid (AA), sodium hydroxide (NaOH), hydrochloric acid (HCl), and dopamine hydrochloride (DA), epinephrine hydrochloride and nor epinephrine hydrochloride were obtained from Sigma. All the chemicals used were of analytical reagent grade and Milli-Q water was used throughout.