20 μg of protein from each sample was loaded on a 4–12% Bolt Bis-Tris gel (Thermo Fisher Scientific #NW04125BOX), samples were run using Bolt MES SDS running buffer (Thermo Fisher Scientific #B0002). Proteins were transferred to Trans-Blot Turbo Mini PVDF membranes (BioRad #1704156) using the mix transfer setting of a Trans-Blot Turbo Transfer System. Membranes were blocked with 5% milk prepared in TBST for an hour at RT. Antibodies were diluted 1:2000 in 5% milk in TBST and incubated overnight at 4 °C. HRP-conjugated secondary antibodies were used at 1:10,000 dilution in 5% milk in TBST and protein bands were imaged using Clarity Max ECL Substrate (BioRad #1705062) and an iBrighty Imaging system.
Ibrighty imaging system
Manufactured by Bio-Rad
The IBrighty Imaging system is a versatile and efficient tool designed to capture high-quality images of various samples. It features advanced optics and a sensitive camera that enable precise visualization and documentation of biological samples, gels, and other specimens.
Automatically generated - may contain errors
2 protocols using ibrighty imaging system
1
Western Blot Protein Analysis Protocol
2
Western Blot Protein Analysis Protocol
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20 μg of protein from each sample was loaded on a 4–12% Bolt Bis-Tris gel (Thermo Fisher Scientific #NW04125BOX), samples were run using Bolt MES SDS running buffer (Thermo Fisher Scientific #B0002). Proteins were transferred to Trans-Blot Turbo Mini PVDF membranes (BioRad #1704156) using the mix transfer setting of a Trans-Blot Turbo Transfer System. Membranes were blocked with 5% milk prepared in TBST for an hour at RT. Antibodies were diluted 1:2000 in 5% milk in TBST and incubated overnight at 4 °C. HRP-conjugated secondary antibodies were used at 1:10,000 dilution in 5% milk in TBST and protein bands were imaged using Clarity Max ECL Substrate (BioRad #1705062) and an iBrighty Imaging system.
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