Scanning electron microscopy (SEM) was used to determine the structural assembly at higher resolution than by optical microscopy, where we specifically determine the fractal dimension, rather than pore sizes or their distribution. Prior to scanning, structures were fixed overnight (4°C) using a 0.1 M phosphate buffer containing 1.5% glutaraldehyde and 1% tannic acid and then dehydrated using a series of ethanol washes and a critical point drying step (Samdri 780 critical point drier, Tousimis, Research Corp., Rockville, MD). Lastly, gold/palladium (60 : 40) was deposited onto the structures, with a thickness of 30 nm, using a DC sputter coater (Auto Conductavac IV, Seevac Inc., Pittsburgh, PA). SEM images were acquired using the Hitachi S-570 microscope (Hitachi, Tokyo, Japan). The mean fractal dimension was computed using the FIJI FracLac plugin.
S 570 microscope
Manufactured by Hitachi
Sourced in Japan
The Hitachi S-570 is a scanning electron microscope (SEM) designed for high-resolution imaging of a wide range of samples. It features a large specimen chamber, a high-resolution electron column, and advanced imaging capabilities to provide detailed, high-quality images of samples at the microscopic level.
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Lab products found in correlation
2 protocols using s 570 microscope
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Structural Characterization via SEM Fractal Analysis
2
Morphological Characterization of Strain LzC2
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Morphological characterization of strain LzC2 T was carried out by phase contrast, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) during the exponential phase of growth in M14 medium. Photographic records of colonies of the strain in M14 medium were also obtained. For phase contrast microscopy, cells were observed in a Nikon Eclipse Ci equipment under 1000x magnification. For SEM, cells were fixed in 2.5% (v/v) glutaraldehyde in marine buffer, pH 7.0 for 2.5 h, dehydrated through a graded ethanol series, critical point-dried and observed with a HITACHI S-570 microscope. For TEM, cells were fixed in 2.5% (v/v) glutaraldehyde in marine buffer (pH 7.2) for 2 h and post-fixed in 1% (v/v) osmium tetroxide in the same buffer for 4 h followed by 1% (v/v) uranyl acetate for 1 h. Cells were dehydrated through a graded ethanol series, followed by propylene oxide treatment and were ultimately embedded in Epon resin. Ultrathin sections were stained for 10 min in 1% (v/v) uranyl acetate and for 10 min in Reynolds lead citrate. The sections were examined using a 100CXII transmission electron microscope (JEOL).
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