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L glutaraldehyde

Manufactured by Merck Group
Sourced in United States

L-glutaraldehyde is a chemical compound used as a fixative and cross-linking agent in various laboratory techniques. It is a colorless liquid with a pungent odor. L-glutaraldehyde is commonly used to preserve and stabilize biological samples for microscopy, immunohistochemistry, and other analytical procedures.

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3 protocols using l glutaraldehyde

1

Cocaine Exposure Assays in Cell Culture

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RPMI 1640, fetal bovine serum (FBS), penicillin/streptomycin, amphotericin B, phosphate-buffered saline (PBS), Hank’s balanced salt solution (HBSS), and L-glutamine were purchased from Media Tech (Herndon, VA). Horse serum (HS) was obtained from Invitrogen (Waltham, MA). Cocaine-HCl (Ecgonine methyl ester benzoate, MW: 339.8), crystal violet, 2′,7′-dichlorofluorescin diacetate, L-glutaraldehyde, trypan blue, N-1-napthylethylenediamine dihydrochloride (NED), rhodamine (Rh)123 and EDTA (ethylene diamine tetraacetic acid) were supplied by Sigma Chemical Company (St. Louis, MO). All other routine chemicals were of analytical grade.
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2

Cytotoxicity Assessment of Methamphetamine

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RPMI 1640, fetal bovine serum (FBS), penicillin/streptomycin sulfate, amphotericin B, phosphate-buffered saline (PBS) and L-glutamine were purchased from Media Tech (Herndon, VA). Crystal violet, L glutaraldehyde, trypan blue, methamphetamine (METH) hydrochloride (MW: 185.69), 2′,7′-dichlorofluorescin diacetate (H2DCFDA), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), and EDTA were supplied by Sigma-Aldrich Company (St. Louis, MO). All other routine chemicals were of analytical grade.
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3

Cocaine-Induced Oxidative Stress Assay

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All chemicals used were of analytical grade. RPMI 1640, fetal bovine serum (FBS), penicillin/streptomycin sulfate, amphotericin B, phosphate-buffered saline (PBS) and L-glutamine were obtained from Media Tech (Herndon, VA, USA). Cocaine (Ecgonine methyl ester benzoate) hydrochloride, crystal violet, dichlorodihydrofluorescin diacetate dye (H2DCFDA), L-glutaraldehyde, 0.5 M EDTA (ethylene diamine tetraacetic acid) solution, 5,5-dithiobis-2-nitrobenzoic acid (DTNB), nicotinamide adenosine dinucleotide phosphate (NADPH), 5-sulfosalicylic acid, NAC and trypan blue were obtained from Sigma-Aldrich (St. Louis, MO, USA) and used according to various protocols.
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