Alcohol dependence was induced by 9 days of daily intraperitoneal (i.p.) injection of EtOH (2 g/kg, 20% w/v in 0.9% saline, or saline control) with the alcohol dehydrogenase inhibitor fomepizole (4-methylpyrazole, 9 mg/kg, Sigma-Aldrich)(31 (link)). Blood alcohol level was assessed from tail bleeds 1h (intoxication) and 24h (withdrawal) after injection on day 6 or just before injection on day 7 using gas chromatography. One female from the naltrexone treatment group was euthanized following adverse effects from alcohol.
Fomepizole
Manufactured by Merck Group
Fomepizole is a laboratory equipment product manufactured by Merck Group. It is a chemical compound used as an antidote for the treatment of ethylene glycol or methanol poisoning. Fomepizole works by inhibiting the enzyme alcohol dehydrogenase, which is responsible for metabolizing these toxic alcohols into more harmful substances.
Automatically generated - may contain errors
2 protocols using fomepizole
1
Alcohol Dependence Induction Protocol
2
Pharmacological Agents for Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dexamethasone, mifepristone, fomepizole, and common reagents were purchased from Sigma-Aldrich. Lipopolysaccharide (E. coli, serotype O111:B4 L2630) was from List Biological Laboratories (Campbell, CA) or from Sigma-Aldrich. Pure anhydrous ethyl alcohol or ethanol (200 proof/100%, Koptec), propanol (Sigma-Aldrich), and isopropanol (2-propanol, Sigma-Aldrich) were obtained commercially.
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