Antibody against drp1

Lactate was obtained from Sigma (St. Louis, MO, USA), Antibody against DRP1(#8570), p-DRP1^S616(#3455), p-IκB-alpha(#2859S), p-P65(#3033S) and P65(#8242) were purchased from Cell Signaling Technology (Boston, MO, USA). Antibody against fibronectin (#15,613–1-AP) and COL1A1(67,288–1-Ig) were purchased from Proteintech (Wuhan, China). Antibody against IκB(#AF5002), IKK(#AF6014), p-IKK(AF3013), ERK(#AF0155), p-ERK(#AF1015), β-Actin(#AF7018) and GAPDH(#AF7021) were purchased from Affinity (Changzhou, China).

Adenine containing a purity 99% was obtained from Amresco (Texas, America). The kits for determination of creatine kinase-MB (CK-MB) and cardiac troponin T (cTnT) were provided by BioSino (Beijing, China). The kits of serum creatinine (CREA) and urea nitrogen (UREA) were produced by Roche Diagnostics GmbH (Mannheim, Germany). Evans blue and triphenyltetrazolium chloride solution (TTC) were bought from Sigma-Aldrich (St. Louis, MO, United States). Tissue mitochondria isolation kit was bought from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Protein concentration was measured using a bicinchoninic acid (BCA) protein assay kit (AR1189, Boster, Wuhan, China). Antibodies against FIS1 (1:500), OPA1 (1:1000), MFN1 (1:1000), and COX IV (1:1000) were purchased from Abcam (Cambridge, United Kingdom). Antibody against DRP1 (1:1000) was purchased from Cell Signaling Technology (Beverly, MA, United States). The GXN was purchased from Yabao Pharmaceutical Group Co. Ltd. (Yuncheng, China, SFDA approval number: Z14020782).