Bovine serum albumin c bsa c

M199 and Eagle’s minimum essential medium (EMEM) were purchased from Lonza (Basel, Switzerland). Fetal calf serum (FCS), human serum, endothelial cell supplement (ECGS), trypsin-EDTA, non-essential amino acids, L-glutamine, anhydrous dimethylsulfoxide (DMSO), Akt inhibitor VIII (Akt VIII), Torin-2, and Hoechst 33342 were from Sigma (Taufkirchen, Germany). bovine serum albumin-C (BSA-C) was obtained from Aurion (Wageningen, The Netherlands) and goat serum from Cell Signaling Technology (Frankfurt, Germany). EDTA-free protease inhibitor cocktail was purchased from Roche Diagnostics (Mannheim, Germany). β-Mercaptoethanol, dithiothreitol (DTT), EDTA, bovine serum albumin, Tween-20, and Triton X-100 were obtained from Carl Roth GmbH (Karlsruhe, Germany). Fluoromount-G was from Southern Biotech (Birmingham, AL, US). MK-8722 was purchased from Aobious (Gloucester, MA, USA). The siRNAs against AMPKα1 or AMPKα2, ACC1, and non-targeting control-siRNA were SMARTpool-siRNAs obtained from Horizon, Dharmacon RNAi, and Gene Expression (Lafayette, CO, USA).

M199 was purchased from Lonza (Verviers, Belgium), fetal calf serum (FCS) and human serum were from Sigma (Taufkirchen, Germany) and endothelial mitogen was purchased from Biomedical Technologies Inc. (Stoughton, MA, USA). The siRNAs against AMPKα1, AMPKα2, ULK1, BECN1 and non-targeting control siRNA were SMARTpool-siRNAs obtained from GE Healthcare, Dharmacon RNAi and Gene Expression (Lafayette, CO, USA). Recombinant human VEGF-165 was obtained from R&D Systems GmbH (Wiesbaden, Germany). Protease inhibitor mixture complete, EDTA-free, was obtained from Roche Diagnostics (Mannheim, Germany) and used in protein lysates, while a protease inhibitor cocktail obtained from Sigma (Taufkirchen, Germany) was administered to protect cytokine degradation in supernatants. Propidium iodide, RNase A, DL-buthionine-(S,R)-sulfoximine (BSO), 5,5′-Dithiobis(2-nitrobenzoic acid) (DTNB), NADPH, 2-deoxyglucose (2-DG), thrombin and aprotinin were purchased from Sigma (Taufkirchen, Germany). Bafilomycin A1 was from Enzo Life Sciences (Lörrach, Germany) and fibrinogen was from Merck/Millipore (Darmstadt, Germany). Bovine serum albumin-C (BSA-C) and goat serum were obtained from Aurion (Wageningen, The Netherlands) and Cell Signaling Technology (Frankfurt, Germany), respectively.