Triethanolamine teoha

The following materials were purchased from Sigma-Aldrich (United States) and used without further purification: sodium hyaluronate (M_n = 100 kDa), glycidyl methacrylate (GMA), tetraethylammonium bromide (TEAB), poly(ethylene glycol) diacrylate (PEG-DA, M_n = 575), acetone, N,N-dimethylformamide (DMF), potassium permanganate (KMnO₄), sodium tetraborate decahydrate (Na₂B₄O₇×10H₂O), concentrated hydrochloric acid (36%), glacial acetic acid, 4-(dimethylamino)benzaldehyde (DMAB), N-acetyl-D-glucosamine (NAG), and hyaluronidase from bovine testes. Flavin mononucleotide (FMN) was obtained from Pharmstandard (Russia). Triethanolamine (TEOHA) was purchased from Merck (United States). 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 97% purity, was obtained from Hangzhou Dingyan Chem Co., Ltd (China). Phosphate buffered saline (PBS), pH 7.4, was prepared by dissolving biotechnology grade PBS tablet (VWR Life Science, Canada) in 100 ml of deionized water. Penicillin–streptomycin (5,000 U/ml and 5,000 µg/ml, respectively) was purchased from PanEco (Russia). Amphotericine B (5,000 µg/ml) was purchased from JSC “Sintez” (Russia).

The following materials were purchased from Sigma-Aldrich (United States): sodium hyaluronate (Mn = 100 kDa), glycidyl methacrylate (GMA), tetraethylammonium bromide, poly(ethylene glycol) diacrylate (PEG-DA, Mn = 575), acetone, N,N-dimethylformamide (DMF), and potassium permanganate (KMnO4). Flavin mononucleotide (FMN) was obtained from Pharmstandard (Russia) and triethanolamine (TEOHA) from Merck (United States). Phosphate buffered saline (PBS, pH 7.4) was prepared by dissolving a biotechnology grade PBS tablet (VWR Life Science, Canada) in 100 ml of deionized water. Penicillin-streptomycin (5000 U/ml and 5000 μg/ml respectively) was purchased from PanEco (Russia). Amphotericin B (5000 μg/ml) was purchased from JSC “Sintez” (Russia).