ATP in BALF was assessed using a Fluorometric Assay Kit (SIGMA ALDRICH, MO, USA). The LDH detection kit (Roche Diagnostics, Indianapolis, IN, USA) was used to assess the cytotoxic effect of different treatments. Data were expressed as the fold changes of different groups versus the control groups. Mouse CXCL1/KC ELISA kit (R&D Systems Inc. Minneapolis, MN, USA) was used to quantify KC in BALF.
Fluorometric assay kit
Manufactured by Merck Group
Sourced in United States
The Fluorometric Assay Kit is a laboratory equipment product that enables the measurement of target analytes using fluorescence-based detection methods. It provides the necessary reagents and components to conduct quantitative analyses in a controlled and standardized manner.
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Lab products found in correlation
3 protocols using fluorometric assay kit
1
Quantification of ATP and Cytotoxicity
2
GTP and ATP Quantification in Diabetic Mice
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BMCs and liver tissues were obtained from WT and db/db mice at 8 and 24 weeks. Equal amounts of protein were measured for GTP quantification using an enzymatic assay (ml027797, MLBio, Shanghai, China). ATP concentration was measured using a commercial Fluorometric Assay kit (213-579-1, Sigma-Aldrich, Saint Louis, US). All procedures were in accordance with the manufacturer’s instructions.
3
Fluorometric Assay for Glucose Uptake
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We used the glucose uptake Fluorometric Assay Kit from Sigma-Aldrich following the manufacturer’s instructions. Briefly, glucose uptake in C2C12 myotubes was measured using a glucose analog, 2-DG. 2-DG uptake was determined by a coupled enzymatic assay in which the 2-DG6P is oxidized, generating NADPH which reacts with the probe to generate a fluorometric signal (λex535/λem587 nm) proportional to the 2-DG taken up by the cells.
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